Microfluidic Cell Culture for Live Biotherapeutic Products Assessment
As the world's leading live biotherapeutics research CRO, Creative Biolabs has focused on next-generation probiotics research for years. We provide expertise and technology in preclinical research and evaluation to simplify every step of development in a faster and more cost-effective way.
Backgrounds
In pharmaceutical research, an adequate cell-based assay scheme to efficiently screen and validate potential drug candidates in the initial stage of drug discovery is crucial. Microfluidic technology is a valuable tool for enabling more in vitro models to capture cellular and organ-level responses for rapid and animal-free risk assessment of new chemicals and drugs.
Microfluidic Cell Culture Systems
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Cell Culture in Suspension and Monolayers
Fluid suspension is the natural environment of several cell types, including mammalian blood cells and yeast cells. Other cell types retain important phenotypic features in monolayer cultures. In monolayers of cultured epithelial cells, cell attachments are usually conserved. Suspension cell cultures are traditionally performed in roller flasks or rotating flasks, whereas monolayer cell cultures are performed in multiwell plates, Petri dishes, or culture flasks.
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Three-Dimensional Cell Culture
In vivo tissue organization and three-dimensional cell culture play an important role in cells' behavior. 3D-cell culture can increase the biological relevance of cell-based models beyond what can be achieved in monolayer cultures.
Fig.1 Cell-based assays on microfluidics for drug screening. (Liu, 2019)
Microfluidic Culture for Live Biotherapeutics Research
Throughout the drug discovery and development processes, microfluidic-based cell culture or cellular assay systems can play critical roles in, for example, pre-clinical studies including toxicity testing, efficacy study, and the study of drug delivery mechanisms.
Drug toxicity assessment is essential for the safe development of new drugs and is usually based on animal and cell models. Toxicity information from preclinical studies gives us confidence in the safety of potential drugs. The development of in vitro cell culture systems that can mimic the response of native liver tissue to drug toxicity is a priority in the field of drug discovery.
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High-throughput Drug Research
The successful implementation of microfluid-based cell culture systems in pharmaceutical research largely depends on their high-throughput capabilities. The gradient-generation function in a microfluidic device enables high-throughput assays to be performed in different formats.
Advantages of Microfluidic Cell Culture
Microfluidics provides a high degree of control over cell culture conditions in various aspects. Microfluidic cell culture devices allow precise control of cell numbers and density in a given area or volume and can provide placement of cells in complex geometries, monitoring with the high spatial and temporal resolution, and their retrieval during or following experiments. Parallel experimental conditions allow enhanced cell-based screening assays such as immunophenotyping to monitor single-cell cytokine production in response to external stimuli.
Creative Biolabs has over 10 years of experience providing complete live biotherapeutics research services for the life sciences and pharmaceutical industries. Our team of experienced scientists is focused on delivering high-quality services on time. If you are interested in our microfluidic culture for live biotherapeutics product assessment, please feel free to contact us for more.
Reference
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Liu, X.; et al. Cell-Based Assays on Microfluidics for Drug Screening. ACS Sens. 2019 Jun 28; 4(6): 1465-1475.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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