Eggerthellaceae Family Probiotics Development Solutions

The Eggerthellaceae family has garnered significant attention in next-generation probiotic research due to its specialized capability to metabolize dietary ellagitannins and ellagic acid into beneficial compounds such as urolithins, notably isolecithine-A. Recognizing the profound potential of these bacteria, Creative Biolabs offers cutting-edge probiotic development services, supporting researchers globally to leverage Eggerthellaceae probiotics for advanced microbiome modulation.

Backgroud of Eggerthellaceae Family

Eggerthellaceae family members, belonging to the Actinobacteria phylum, include anaerobic bacteria instrumental in the biotransformation of dietary polyphenols. This family uniquely metabolizes ellagic acid—derived from ellagitannins found in pomegranates, berries, and nuts—into bioactive urolithins. Among these metabolites, isolecithine-A stands out due to its robust biological activity, significantly enhancing polyphenol benefits through improved bioavailability.

The transformation from ellagic acid to isolecithine-A involves enzymatic pathways mediated by specific bacterial strains, highlighting the importance of these microbes in gut microbiota research and microbiome modulation strategies.

Eggerthellaceae Probiotics Development Solutions at Creative Biolabs

The shift towards next-generation probiotics emphasizes the development of microbial strains with targeted functionalities beneficial to host health. Eggerthellaceae probiotics exemplify this paradigm through their specialized ability to produce urolithins, significantly influencing gut microbial ecology and metabolic profiles. At Creative Biolabs, we provide integrated probiotic development solutions, leveraging advanced microbiological, biochemical, and genomic technologies to maximize the potential of Eggerthellaceae probiotics:

Advanced Technologies Empowering Eggerthellaceae Probiotic Solutions

To support the high-value development of urolithin-producing probiotics, Creative Biolabs integrates a set of critical technologies designed for precise strain validation and functional profiling. Each selected technology is directly relevant to the biological characteristics and research utility of Eggerthellaceae family members.

Adhesion Assays for Probiotics

Eggerthellaceae strains are strictly anaerobic and often sensitive to external conditions, making their ability to adhere to intestinal epithelium a crucial indicator of probiotic potential. Adhesion assays are used to evaluate the interaction between candidate strains and Caco-2 or HT-29 epithelial cells, determining colonization capacity and persistence in the gut—key for metabolic activity such as ellagic acid biotransformation.

Anti-inflammatory Assays

Since urolithins, especially isourolithin A, have demonstrated anti-inflammatory effects in various models, it's essential to verify whether the probiotic strain contributes to anti-inflammatory activity. We use TNF-α/LPS-stimulated macrophage models (e.g., RAW264.7) to assess NO, IL-6, and IL-1β secretion. This step links microbial metabolism to host immune modulation, a hallmark of next-generation probiotics.

Germ-Free Mouse Model

To validate in vivo colonization and urolithin production, Creative Biolabs integrates fecal microbiota sampling from germ-free or antibiotic-treated mice. These models allow controlled colonization with specific Eggerthellaceae strains. Fecal sampling is followed by metabolite profiling (e.g., LC-MS of urolithin A) and 16S rRNA tracking of colonization efficiency.

SCFA Analysis via HPLC

Short-chain fatty acids (SCFAs), though not direct products of ellagic acid metabolism, are crucial functional markers of probiotic metabolic activity and microbiome modulation. Eggerthellaceae may indirectly influence SCFA profiles through cross-feeding with other gut microbes. GC and HPLC are used to quantify acetate, propionate, and butyrate in fermentation supernatants.

Survival Assays for Probiotic Strains

The acid and bile tolerance of Eggerthellaceae strains is critical for their function as oral probiotics. Survival assays simulate gastric juice (pH ~2.0) and bile salt environments to assess strain viability. This directly informs formulation strategies and delivery route design for research applications.

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Eggerthellaceae Related Products

Complementing our probiotic development solutions, Creative Biolabs also provides an array of specialized Eggerthellaceae-related products:

CAT	Product Name	Target	Product Overview
LBST-074FG	Eggerthellaceae sp.	Eggerthellaceae	The Eggerthellaceae is a family of Gram-positive, rod- or coccus-shaped Actinobacteria. It is the sole family within the order Eggerthellales.
LBST-075FG	Adlercreutzia equolifaciens	Eggerthellaceae	Adlercreutzia equolifaciens is an anaerobe, mesophilic, Gram-positive Actinobacterium that was isolated from human faeces.
LBST-076FG	Adlercreutzia equolifaciens; 54925	Eggerthellaceae	Adlercreutzia equolifaciens is an anaerobe, mesophilic, Gram-positive Actinobacterium that was isolated from human faeces.
LBST-077FG	Cryptobacterium curtum; 45151 B	Eggerthellaceae	Cryptobacterium curtum was isolated from human blood. It is a Gram-positive, obligately anaerobic, non-spore-forming, and rod-shaped bacteria.
LBST-078FG	Cryptobacterium curtum; 55333T	Eggerthellaceae	Cryptobacterium curtum was isolated from human periodontal pocket. It is a Gram-positive, obligately anaerobic, non-spore-forming, and rod-shaped bacteria.
LBST-079FG	Cryptobacterium curtum; 12-3	Eggerthellaceae	Cryptobacterium curtum was isolated from peridontal pocket of an adult with peridontal disease. It is a Gram-positive, obligately anaerobic, non-spore-forming, and rod-shaped bacteria.
LBST-080FG	Denitrobacterium detoxificans; NPOH2	Eggerthellaceae	Denitrobacterium detoxificans was isolated from bovine rumen contents. It is the only described species in the Denitrobacterium genus.
LBST-081FG	Gordonibacter pamelaeae; 55131T	Eggerthellaceae	Gordonibacter pamelaeae is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from human intestinal biopsy.
LBST-082FG	Gordonibacter urolithinfaciens; 64261T	Eggerthellaceae	Gordonibacter urolithinfaciens was isolated from human feces. It is a Gram-positive bacteria belonging to the recent family Eggerthellaceae.
LBST-083FG	Gordonibacter urolithinfaciens; 27213	Eggerthellaceae	Gordonibacter urolithinfaciens was isolated from human feces of a healthy volunteer. It is a Gram-positive bacteria belonging to the recent family Eggerthellaceae.
LBST-084FG	Gordonibacter pamelaeae	Eggerthellaceae	Gordonibacter pamelaeae is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from patient with active Crohn's disease.
LBST-085FG	Paraeggerthella hongkongensis	Eggerthellaceae	Paraeggerthella hongkongensis is an anaerobe, mesophilic bacterium that was isolated from blood from a patient with perianal abscess.
LBST-086FG	Slackia equolifaciens	Eggerthellaceae	Slackia equolifaciens was isolated from human feces. It is an equol-producing bacterium.
LBST-087FG	Slackia exigua; 36663	Eggerthellaceae	Slackia exigua was isolated from human oral abscess. It is a Gram-positive bacterium.
LBST-088FG	Slackia exigua; 445bbT	Eggerthellaceae	Slackia exigua was isolated from human necrotic pulp of tooth. It is a Gram-positive bacterium.
LBST-089FG	Slackia equolifaciens; 58231T	Eggerthellaceae	Slackia equolifaciens was isolated from human feces. It is an equol-producing bacterium.

Applications and Benefits in Microbiome Research

Utilizing Eggerthellaceae probiotics developed by Creative Biolabs, researchers can explore key microbiota interactions, dietary polyphenol bioavailability enhancement, and microbial ecosystem modulation. These probiotics are particularly beneficial in:

• Investigating polyphenol metabolism
• Modulating gut microbiota composition
• Exploring microbial influence on cellular processes and aging

Eggerthellaceae probiotics represent an innovative frontier in microbiome modulation technologies, essential for advanced polyphenol and gut microbiota research. With comprehensive probiotic development solutions and specialized related products from Creative Biolabs, researchers are well-equipped to explore these potent microbes and their significant potential in scientific discovery.

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