Gordonibacter pamelaeae is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from human intestinal biopsy.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information | |
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Product Overview | Gordonibacter pamelaeae is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from human intestinal biopsy. |
Target | Eggerthellaceae |
Genus | Gordonibacter |
Application | Study and research |
Culture Medium | Chocolate agar |
Culture Conditions | 37°C; Anaerobic |
Source | Human intestinal biopsy, inflammatory bowel disease |
Risk Group | 2 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
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Introduction | The Eggerthellaceae are a family of Gram-positive, rod- or coccus-shaped Actinobacteria. It is the sole family within the order Eggerthellales. Members of this family are mostly anaerobic, non-motile, asaccharolytic and do not form spores. Eggerthellaceae species are commonly isolated from human and animal faeces and other human sources such as the colon, vagina, oral cavity and blood. |
Alternative Names | Gordonibacter pamelaeae; 55131T |
Gordonibacter pamelaeae; 55131T is typically introduced into animal models via oral gavage, ensuring precise dosage and delivery. This method allows researchers to study its effects on gut microbiota and host health in a controlled and reproducible manner.
To revive Gordonibacter pamelaeae; 55131T from a frozen state, thaw the cryovial rapidly at 37°C and immediately transfer the contents to an anaerobic growth medium. Incubate under anaerobic conditions to ensure optimal recovery and viability of the bacteria.
Colonization efficiency can be assessed using quantitative PCR (qPCR) to measure bacterial DNA in fecal samples, along with culture-based methods to enumerate colony-forming units (CFUs). These approaches provide insights into the persistence and activity of Gordonibacter pamelaeae; 55131T in the gut.
The metabolic activity of Gordonibacter pamelaeae; 55131T is studied using techniques such as gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy to analyze metabolite production. These methods help elucidate the metabolic pathways and functions of the bacterium.
Accurate identification involves using molecular techniques such as 16S rRNA gene sequencing and species-specific PCR assays. These methods distinguish Gordonibacter pamelaeae; 55131T from other bacteria in mixed cultures, ensuring precise identification and characterization.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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