Slackia equolifaciens was isolated from human feces. It is an equol-producing bacterium.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information
Product Overview
Slackia equolifaciens was isolated from human feces. It is an equol-producing bacterium.
Target
Eggerthellaceae
Genus
Slackia
Application
Study and research
Culture Medium
Chocolate medium
Culture Conditions
37°C; Anaerobic
Source
Human feces
Risk Group
1
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
The Eggerthellaceae are a family of Gram-positive, rod- or coccus-shaped Actinobacteria. It is the sole family within the order Eggerthellales. Members of this family are mostly anaerobic, non-motile, asaccharolytic and do not form spores. Eggerthellaceae species are commonly isolated from human and animal faeces and other human sources such as the colon, vagina, oral cavity and blood.
Alternative Names
Slackia equolifaciens; 58231T
FAQs Customer Reviews Related Products
How can Slackia equolifaciens; 58231T be introduced into in vivo models for research purposes?
Slackia equolifaciens; 58231T can be introduced into in vivo models via oral gavage, ensuring precise dosage and delivery. This method allows researchers to study its effects on gut microbiota composition and host health in a controlled experimental environment.
What are the recommended procedures for preparing Slackia equolifaciens; 58231T for in vitro experiments?
For in vitro experiments, prepare Slackia equolifaciens; 58231T by culturing it in an anaerobic chamber using a suitable growth medium. Ensure the culture reaches the desired optical density (typically OD600 of 0.6-0.8) before using it in experimental setups.
How can the presence of Slackia equolifaciens; 58231T be confirmed in experimental samples?
The presence of Slackia equolifaciens; 58231T can be confirmed using molecular techniques such as 16S rRNA gene sequencing or species-specific PCR assays. These methods provide accurate identification and quantification of the bacterium in various samples.
What are the considerations for scaling up the culture of Slackia equolifaciens; 58231T for large-scale studies?
Scaling up requires maintaining anaerobic conditions in larger bioreactors, ensuring a consistent nutrient supply, and monitoring growth parameters closely. Optimization of these conditions is crucial for achieving high biomass yields while preserving bacterial viability and functionality.
What methods can be used to assess the immunomodulatory effects of Slackia equolifaciens; 58231T?
Assess immunomodulatory effects by co-culturing Slackia equolifaciens; 58231T with immune cells and measuring cytokine production using ELISA. Flow cytometry can also be employed to analyze changes in immune cell populations, providing comprehensive insights into the bacterium's impact on the immune system.
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