Gordonibacter pamelaeae is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from patient with active Crohn's disease.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information
Product Overview
Gordonibacter pamelaeae is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from patient with active Crohn's disease.
Target
Eggerthellaceae
Genus
Gordonibacter
Strain Designation
7-10-1-b
Application
Study and research
Culture Medium
Chocolate agar
Culture Conditions
37°C; Anaerobic
Source
Patient with active Crohn's disease, colon, sigmoid region
Risk Group
1
Genbank Accession Numbers
16S rRNA gene: AM886059
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
The Eggerthellaceae are a family of Gram-positive, rod- or coccus-shaped Actinobacteria. It is the sole family within the order Eggerthellales. Members of this family are mostly anaerobic, non-motile, asaccharolytic and do not form spores. Eggerthellaceae species are commonly isolated from human and animal faeces and other human sources such as the colon, vagina, oral cavity and blood.
Alternative Names
Gordonibacter pamelaeae
FAQs Customer Reviews Related Products
How should Gordonibacter pamelaeae be revived from a frozen state for experimental use?
To revive Gordonibacter pamelaeae from a frozen state, thaw the cryovial rapidly at 37°C and immediately transfer the contents to an anaerobic growth medium. Incubate under anaerobic conditions to ensure optimal recovery and viability of the bacteria.
What methods can be used to assess the colonization efficiency of Gordonibacter pamelaeae in the gut?
Colonization efficiency can be assessed by administering Gordonibacter pamelaeae to animal models and analyzing fecal samples using quantitative PCR (qPCR) and culture-based methods to measure bacterial load and distribution within the gut.
What are the recommended procedures for preparing Gordonibacter pamelaeae for in vitro experiments?
For in vitro experiments, prepare Gordonibacter pamelaeae by culturing it in an anaerobic chamber using a suitable growth medium. Ensure the culture reaches the desired optical density (typically OD600 of 0.6-0.8) before using it in experimental setups.
How can the effects of Gordonibacter pamelaeae on host immune responses be measured?
The effects on host immune responses can be measured using techniques such as flow cytometry to analyze immune cell populations, ELISA to quantify cytokine levels, and transcriptomic analyses to study gene expression changes in response to the bacterium.
How can the impact of Gordonibacter pamelaeae on gut microbiota composition be studied?
The impact can be studied by administering Gordonibacter pamelaeae to animal models and using metagenomic sequencing to analyze changes in gut microbiota composition. This provides insights into how the bacterium interacts with and influences the microbial community.
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