Prevotella intermedia is a Gram-negative, obligate anaerobic pathogenic bacterium involved in periodontal infections, including gingivitis and periodontitis, and often found in acute necrotizing ulcerative gingivitis. Prevotella intermedia use steroid hormones as growth factors, so their numbers are higher in pregnant women.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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LBST-008FG |
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Product Information | |
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Product Overview | Prevotella intermedia is a Gram-negative, obligate anaerobic pathogenic bacterium involved in periodontal infections, including gingivitis and periodontitis, and often found in acute necrotizing ulcerative gingivitis. Prevotella intermedia use steroid hormones as growth factors, so their numbers are higher in pregnant women. |
Target | Prevotella |
Genus | Prevotella |
Application | Study and research |
Type Strain | Yes |
Culture Medium | Columbia blood agar |
Culture Conditions | 37°C; Anaerobic |
Source | Empyema |
Risk Group | 1 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
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Introduction | Prevotella is a genus of anaerobic Gram-negative bacteria of the Bacteroidetes phylum, which also includes the clinically important genera Bacteroides and Porphyromonas. Prevotella strains are classically considered commensal bacteria due to their extensive presence in the healthy human body and their rare involvement in infections. Prevotella spp. are members of the oral, vaginal, and gut microbiota and are often recovered from anaerobic infections of the respiratory tract. Prevotella spp. predominate in periodontal disease and periodontal abscesses. Regarding Prevotella, studies focused on the associations between the diverse species, genome and habitats with dietary patterns, health and disease. Evidence revealed beneficial effects of some Prevotella strains in the gut such as improving CVD risk factor profile and glucose metabolism. Most studies reported that vegetarians harbour higher numbers of Prevotella species as well as a high Prevotella/Bacteroides ratio compared with non-vegetarians; omnivores. Nevertheless, there is a need for more studies in humans to ascertain a causal and potential disease-triggering role for Prevotella and more in-depth metagenomic studies are needed in order to reveal the health- or disease-modulating properties. |
Alternative Names | Prevotella intermedia |
Prevotella intermedia should be cultured under anaerobic conditions at 37°C. Use media such as Brain Heart Infusion (BHI) broth or agar, supplemented with hemin and vitamin K1, to support optimal growth and viability.
Prevotella intermedia should be stored at -80°C in a cryoprotectant solution, such as 15% glycerol. This storage method ensures the viability of the bacterium. When ready to use, thaw rapidly and transfer immediately to anaerobic media.
Isolation of Prevotella intermedia involves homogenizing the sample (e.g., dental plaque or tissue), performing serial dilutions, and plating on selective media like Wilkins-Chalgren agar. Incubate anaerobically at 37°C for 48-72 hours. Identification can be confirmed using molecular methods such as PCR.
Common assays include protease activity tests to assess virulence factors, adhesion assays to evaluate the bacterium's ability to adhere to host tissues, and cytokine profiling to study the immune response. These assays help understand the role of Prevotella intermedia in periodontal disease and other infections.
Quantification of Prevotella intermedia in microbial communities can be achieved using real-time PCR with species-specific primers. This method allows for precise detection and quantification within complex samples, facilitating detailed population analysis.
Interpain A destroys bactericidal activity of NHS
The study explores the role of Interpain A in protecting P. intermedia against the human immune system. P. intermedia is a significant periodontal pathogen known for its resistance to the human complement system, crucial for innate immunity. The study demonstrates that Interpain A, a cysteine protease produced by P. intermedia, effectively degrades the complement factor C3, thus inhibiting all three complement pathways.
Experimental data showed that when human serum was incubated with recombinant Interpain A, there was a notable decrease in the serum's bactericidal activity. Specifically, a clinical strain of P. intermedia expressing Interpain A exhibited higher serum resistance compared to a strain lacking this protease. This resistance was significantly reduced when the protease activity was inhibited by a cysteine protease inhibitor, E64. The presence of the Interpain A gene was confirmed in the majority of P. intermedia strains isolated from periodontitis patients, emphasizing its role in immune evasion. The study highlights the importance of Interpain A in periodontal pathogen survival and suggests its potential as a target for therapeutic interventions.
Potempa, Michal, et al. "Interpain A, a cysteine proteinase from Prevotella intermedia, inhibits complement by degrading complement factor C3." PLoS pathogens 5.2 (2009): e1000316.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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