Prevotella intermedia is a Gram-negative, obligate anaerobic pathogenic bacterium involved in periodontal infections, including gingivitis and periodontitis, and often found in acute necrotizing ulcerative gingivitis. Prevotella intermedia use steroid hormones as growth factors, so their numbers are higher in pregnant women.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
CAT
Size
Price
Quantity
LBST-008FG
Product Information
Product Overview
Prevotella intermedia is a Gram-negative, obligate anaerobic pathogenic bacterium involved in periodontal infections, including gingivitis and periodontitis, and often found in acute necrotizing ulcerative gingivitis. Prevotella intermedia use steroid hormones as growth factors, so their numbers are higher in pregnant women.
Target
Prevotella
Genus
Prevotella
Application
Study and research
Type Strain
Yes
Culture Medium
Columbia blood agar
Culture Conditions
37°C; Anaerobic
Source
Empyema
Risk Group
1
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Prevotella is a genus of anaerobic Gram-negative bacteria of the Bacteroidetes phylum, which also includes the clinically important genera Bacteroides and Porphyromonas. Prevotella strains are classically considered commensal bacteria due to their extensive presence in the healthy human body and their rare involvement in infections. Prevotella spp. are members of the oral, vaginal, and gut microbiota and are often recovered from anaerobic infections of the respiratory tract. Prevotella spp. predominate in periodontal disease and periodontal abscesses. Regarding Prevotella, studies focused on the associations between the diverse species, genome and habitats with dietary patterns, health and disease. Evidence revealed beneficial effects of some Prevotella strains in the gut such as improving CVD risk factor profile and glucose metabolism. Most studies reported that vegetarians harbour higher numbers of Prevotella species as well as a high Prevotella/Bacteroides ratio compared with non-vegetarians; omnivores. Nevertheless, there is a need for more studies in humans to ascertain a causal and potential disease-triggering role for Prevotella and more in-depth metagenomic studies are needed in order to reveal the health- or disease-modulating properties.
Alternative Names
Prevotella intermedia
FAQsPublished Data Customer Reviews Related Products
What are the optimal conditions for culturing Prevotella intermedia?
Prevotella intermedia should be cultured under anaerobic conditions at 37°C. Use media such as Brain Heart Infusion (BHI) broth or agar, supplemented with hemin and vitamin K1, to support optimal growth and viability.
How should Prevotella intermedia be stored before use in experiments?
Prevotella intermedia should be stored at -80°C in a cryoprotectant solution, such as 15% glycerol. This storage method ensures the viability of the bacterium. When ready to use, thaw rapidly and transfer immediately to anaerobic media.
What methods can be used to isolate Prevotella intermedia from clinical samples?
Isolation of Prevotella intermedia involves homogenizing the sample (e.g., dental plaque or tissue), performing serial dilutions, and plating on selective media like Wilkins-Chalgren agar. Incubate anaerobically at 37°C for 48-72 hours. Identification can be confirmed using molecular methods such as PCR.
What assays are commonly used to study the pathogenicity of Prevotella intermedia?
Common assays include protease activity tests to assess virulence factors, adhesion assays to evaluate the bacterium's ability to adhere to host tissues, and cytokine profiling to study the immune response. These assays help understand the role of Prevotella intermedia in periodontal disease and other infections.
How can Prevotella intermedia be quantified in microbial communities?
Quantification of Prevotella intermedia in microbial communities can be achieved using real-time PCR with species-specific primers. This method allows for precise detection and quantification within complex samples, facilitating detailed population analysis.
Interpain A destroys bactericidal activity of NHS
The study explores the role of Interpain A in protecting P. intermedia against the human immune system. P. intermedia is a significant periodontal pathogen known for its resistance to the human complement system, crucial for innate immunity. The study demonstrates that Interpain A, a cysteine protease produced by P. intermedia, effectively degrades the complement factor C3, thus inhibiting all three complement pathways. Experimental data showed that when human serum was incubated with recombinant Interpain A, there was a notable decrease in the serum's bactericidal activity. Specifically, a clinical strain of P. intermedia expressing Interpain A exhibited higher serum resistance compared to a strain lacking this protease. This resistance was significantly reduced when the protease activity was inhibited by a cysteine protease inhibitor, E64. The presence of the Interpain A gene was confirmed in the majority of P. intermedia strains isolated from periodontitis patients, emphasizing its role in immune evasion. The study highlights the importance of Interpain A in periodontal pathogen survival and suggests its potential as a target for therapeutic interventions.
Potempa, Michal, et al. "Interpain A, a cysteine proteinase from Prevotella intermedia, inhibits complement by degrading complement factor C3." PLoS pathogens 5.2 (2009): e1000316. Distributed under Open Access license CC BY 4.0, without modification.
Reliable Pathogenicity Studies
Using Prevotella intermedia from Creative Biolabs has greatly enhanced our pathogenicity studies. The strain's consistent performance in protease activity assays provides crucial insights into its role in periodontal infections.
High-Quality Molecular Identification
Creative Biolabs' Prevotella intermedia allows precise molecular identification. PCR and sequencing confirm the strain's identity, ensuring accurate and reproducible results in microbiome analysis.
Consistent Performance in Adhesion Assays
The consistency of Creative Biolabs' Prevotella intermedia in adhesion assays is remarkable. This strain has been essential in studying bacterial adhesion to host tissues, offering reliable data for our research.
Excellent for Immune Response Studies
Using Prevotella intermedia from Creative Biolabs has improved our immune response studies. The strain's impact on cytokine profiling helps us understand its role in inflammatory processes.
Robust and Reliable
The robust and reliable performance of Creative Biolabs' Prevotella intermedia has been invaluable in our studies. This strain consistently delivers high-quality results, making it a top choice for research on microbial interactions.
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