Prevotella histicola is Gram-negative bacillus that are obligately anaerobic, vary in pigmentation and are non-motile.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
CAT
Size
Price
Quantity
LBST-009FG
Product Information
Product Overview
Prevotella histicola is Gram-negative bacillus that are obligately anaerobic, vary in pigmentation and are non-motile.
Target
Prevotella
Genus
Prevotella
Application
Study and research
Type Strain
Yes
Culture Medium
EG/Columbia blood agar
Culture Conditions
37°C; Anaerobic
Risk Group
1
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Prevotella is a genus of anaerobic Gram-negative bacteria of the Bacteroidetes phylum, which also includes the clinically important genera Bacteroides and Porphyromonas. Prevotella strains are classically considered commensal bacteria due to their extensive presence in the healthy human body and their rare involvement in infections. Prevotella spp. are members of the oral, vaginal, and gut microbiota and are often recovered from anaerobic infections of the respiratory tract. Prevotella spp. predominate in periodontal disease and periodontal abscesses. Regarding Prevotella, studies focused on the associations between the diverse species, genome and habitats with dietary patterns, health and disease. Evidence revealed beneficial effects of some Prevotella strains in the gut such as improving CVD risk factor profile and glucose metabolism. Most studies reported that vegetarians harbour higher numbers of Prevotella species as well as a high Prevotella/Bacteroides ratio compared with non-vegetarians; omnivores. Nevertheless, there is a need for more studies in humans to ascertain a causal and potential disease-triggering role for Prevotella and more in-depth metagenomic studies are needed in order to reveal the health- or disease-modulating properties.
Alternative Names
Prevotella histicola
FAQs Customer Reviews Related Products
What are the optimal conditions for culturing Prevotella histicola?
Prevotella histicola should be cultured under anaerobic conditions at 37°C. Suitable media include Brain Heart Infusion (BHI) broth or agar, supplemented with hemin and vitamin K1. These conditions support optimal growth and viability of this Gram-negative, non-motile bacterium.
How should Prevotella histicola be stored before use in experiments?
Prevotella histicola should be stored at -80°C in a cryoprotectant solution, such as 15% glycerol. This method ensures the preservation of the bacterium's viability. For optimal recovery, thaw the culture rapidly and transfer it immediately to anaerobic media.
What methods are recommended for isolating Prevotella histicola from clinical samples?
Isolation of Prevotella histicola involves homogenizing the sample (e.g., oral or gut tissue), performing serial dilutions, and plating on selective media like Wilkins-Chalgren agar. Incubate anaerobically at 37°C for 48-72 hours. Identification can be confirmed using molecular methods such as PCR.
What assays are commonly used to study the functional impact of Prevotella histicola on health?
Common assays include adhesion assays to evaluate the bacterium's ability to adhere to host tissues, protease activity tests to assess its role in tissue degradation, and cytokine profiling to study its impact on immune response. These assays help in understanding the role of Prevotella histicola in gut health and disease mechanisms.
How can Prevotella histicola be quantified in microbial communities?
Quantification of Prevotella histicola in microbial communities can be achieved using real-time PCR with species-specific primers. This method allows precise detection and quantification within complex samples, facilitating detailed population analysis.
Superior Quality and Performance
Creative Biolabs' Prevotella histicola Downes et al. 2008 offers exceptional quality. Its robust performance in anaerobic cultures has significantly enhanced our microbiota research, providing reliable and reproducible results.
High Viability and Recovery
The high viability and recovery rate of Creative Biolabs' Prevotella histicola from cryopreservation have been remarkable. This strain's robust growth ensures reliable data for our gut microbiota studies.
Precise Molecular Identification
Creative Biolabs' Prevotella histicola allows precise molecular identification. PCR and sequencing confirm the strain's identity, ensuring accurate and reproducible results in microbiome analysis.
Consistent Results in Immune Response Studies
The strain has provided consistent results in our immune response studies. Its role in modulating cytokine profiles is crucial for understanding inflammatory processes in gut health research.
Excellent for Genomic Studies
Using Prevotella histicola from Creative Biolabs has enhanced our genomic studies. The strain's high-quality DNA extraction and sequencing have facilitated detailed analysis of its genetic makeup and functions.
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