Datasheet

Prevotella hominis (CAT#: LBST-006FG)

Prevotella hominis cells are Gram-negative, non-spore-forming, nonmotile rods and strictly anaerobic. Colonies are approximately 0.5-1mm in diameter, slightly translucent, circular, entire, slightly convex and smooth after 2 days at 37 °C on modified GAM agar plates under anaerobic conditions.

For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.

Product Information
Product Overview Prevotella hominis cells are Gram-negative, non-spore-forming, nonmotile rods and strictly anaerobic. Colonies are approximately 0.5-1mm in diameter, slightly translucent, circular, entire, slightly convex and smooth after 2 days at 37 °C on modified GAM agar plates under anaerobic conditions.
Target Prevotella
Genus Prevotella
Application Study and research
Culture Medium GAM
Culture Conditions 37°C; Anaerobic
Source Human feces, Taiwan
Risk Group 2
Product Format Freeze-dried
Packaging Ampoule tube
Storage -80°C
Shelf Life 6 years
Target Introduction
Introduction Prevotella is a genus of anaerobic Gram-negative bacteria of the Bacteroidetes phylum, which also includes the clinically important genera Bacteroides and Porphyromonas. Prevotella strains are classically considered commensal bacteria due to their extensive presence in the healthy human body and their rare involvement in infections. Prevotella spp. are members of the oral, vaginal, and gut microbiota and are often recovered from anaerobic infections of the respiratory tract. Prevotella spp. predominate in periodontal disease and periodontal abscesses. Regarding Prevotella, studies focused on the associations between the diverse species, genome and habitats with dietary patterns, health and disease. Evidence revealed beneficial effects of some Prevotella strains in the gut such as improving CVD risk factor profile and glucose metabolism. Most studies reported that vegetarians harbour higher numbers of Prevotella species as well as a high Prevotella/Bacteroides ratio compared with non-vegetarians; omnivores. Nevertheless, there is a need for more studies in humans to ascertain a causal and potential disease-triggering role for Prevotella and more in-depth metagenomic studies are needed in order to reveal the health- or disease-modulating properties.
Alternative Names Prevotella hominis
FAQs Customer Reviews Related Products
  1. What is the recommended medium for culturing Prevotella hominis Liou et al.?

    Prevotella hominis should be cultured using Brain Heart Infusion (BHI) broth or agar supplemented with hemin and vitamin K1. This medium provides the necessary nutrients and conditions to support the growth and viability of this anaerobic bacterium​.

  2. How should Prevotella hominis be revived from a frozen state?

    To revive Prevotella hominis from a frozen state, rapidly thaw the vial in a 37°C water bath and immediately transfer the contents to pre-reduced anaerobic media. Incubate under anaerobic conditions at 37°C to ensure optimal recovery and growth.

  3. What methods can be used to confirm the identity of Prevotella hominis in a sample?

    Confirm the identity of Prevotella hominis using molecular techniques such as PCR with species-specific primers. Additionally, sequencing of the 16S rRNA gene can provide accurate identification. These methods ensure precise detection and validation of the strain.

  4. How can Prevotella hominis be quantified in gut microbiota studies?

    Quantification of Prevotella hominis in gut microbiota studies can be achieved using real-time PCR with species-specific primers. This technique allows precise detection and quantification of Prevotella hominis within complex microbial communities, facilitating detailed population analysis​.

  5. What assays are commonly used to evaluate the functional impact of Prevotella hominis on gut health?

    Common assays include gut barrier integrity tests using transepithelial electrical resistance (TEER), cytokine profiling for immune response analysis, and microbial profiling using 16S rRNA sequencing. These assays help in understanding the role of Prevotella hominis in gut microbiota and health.

  • Outstanding Culture Viability
    The culture viability of Creative Biolabs' Prevotella hominis is exceptional. The strain revived quickly from frozen storage, ensuring reliable and consistent growth for our gut microbiota studies​.
  • Reliable for Molecular Identification
    The molecular identification of Prevotella hominis from Creative Biolabs was precise and straightforward. PCR and sequencing confirmed its identity, providing confidence in our microbiome profiling studies.
  • Consistent Results in Functional Assays
    Using Creative Biolabs' Prevotella hominis, we achieved consistent results in gut barrier integrity and cytokine profiling assays. This strain has been crucial in studying the impact of gut bacteria on host immune responses​.
  • High-Quality Storage Stability
    The storage stability of Creative Biolabs' Prevotella hominis is impressive. The strain remained viable after long-term storage at -80°C, ensuring reliable performance in subsequent experiments​.
  • Effective for Microbial Interaction Studies
    The Prevotella hominis strain from Creative Biolabs was highly effective in our microbial interaction studies. Its consistent performance allowed us to investigate complex interactions within the gut microbiota.

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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.

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