Lactobacillus johnsonii Powder (CAT#: LBSX-0522-GF3)

Lactobacillus johnsonii requires anaerobic conditions and a temperature range of 37°C to 42°C for optimal growth. It is important to use a suitable growth medium, such as MRS broth, which provides the necessary nutrients for the bacteria to thrive. Ensuring these conditions will maximize bacterial viability and activity.

For in vivo studies, Lactobacillus johnsonii powder should be reconstituted in a sterile saline or buffer solution to achieve the desired concentration. The suspension should then be administered to the test subjects, typically via oral gavage, to ensure accurate dosing. It is crucial to maintain sterility throughout the preparation and administration process to avoid contamination.

The dosage of Lactobacillus johnsonii powder is determined based on the specific experimental design and objectives. Factors such as the test subject species, age, and health status, as well as the desired outcome, influence the dosage. Commonly, researchers use dosages ranging from 10^7 to 10^9 CFU per dose. Accurate dosage determination is essential for achieving reliable and reproducible results.

Common analytical methods include plate counts for viability assessment, qPCR for quantifying bacterial DNA, and flow cytometry for evaluating cellular interactions. Additionally, researchers often use ELISA to measure immune responses and histological analysis to assess tissue changes. These methods provide comprehensive insights into the probiotic's efficacy and mechanisms of action.

The viability of Lactobacillus johnsonii powder is typically assessed using microbiological methods such as plate counts on selective media and flow cytometry. These techniques help determine the number of live bacteria in the powder, ensuring it meets the required CFU for effective use in research. Regular viability testing is crucial for maintaining product quality and performance.