Lab-scale Production of Microbial Freeze-dried Powder Services

Creative Biolabs is an expert in developing live biotherapeutic solutions, offering flexible development services and experience tailored to each project.

Backgrounds of Freeze-drying

Freeze drying is commonly used to remove water to enhance the storage stability of probiotics. The use of freeze-drying methods and the key parameters of this process are critical in providing high viability levels. In freeze drying, the product is frozen below the critical temperature of the formulation. The freezing process is then followed by primary drying, in which chamber pressure is reduced, shelf temperature is usually increased, and unbound water is removed by sublimation. Finally, a secondary drying step is carried out to remove the bound water by desorption, and the product gradually returns to the ambient temperature. In the freeze-drying technique, the important factors including pH of the medium, the content of protective compounds, prehistory of biomass, initial cell concentration, freezing rate, and temperature should be controlled. Osmotic shock and membrane damage are key and important factors in the loss of activity during freeze-drying, which is caused by intracellular icing and recrystallization. A variety of protectants have been added to drying media before the processes of freeze drying to protect the viability of probiotics during dehydration.

Applications of Freeze-drying Technology

Lyophilization is a common process employed to preserve bacteria, either during concentrated culture production in the food and pharmaceutical industries or for culture collections. With in-depth research on the treatment of diseases with probiotics, the development of biomaterial-based encapsulated probiotics can significantly improve their viability and efficiently treat specific diseases or repair tissue. Freeze drying has been the classic method of producing dried bacterial powder. There are many industrial applications for equally preserved microorganisms. Microbiological quality control is necessary for the pharmaceutical, food, and beverage industries. The freeze-drying technology has been used in the preservation of bacterial strains for several applications, mainly in the production of probiotics for the treatment of gastrointestinal disorders.

Packaging and Storage

The method of storage and the packaging material and methodology will influence the shelf life of the dried product. Common reactive agents to decrease the shelf life of products include; oxygen, moisture, light, microbial contamination, and elevated temperatures. Therefore, the packaging material will be a different type of barrier for these reactants. Generally, freeze-dried products are stored in ampoules or glass bottles. For dry products, there are other options such as high-barrier plastic bags and blister packaging.

Creative Biolabs' Lab-scale Production of Microbial Freeze-dried Powder Services

Creative Biolabs offers freeze-drying services for clients who wish to preserve their bacterial or fungal cultures and maintain their properties. Using this approach, you can save the culture in one of two formats:

  • Glass Ampoules

The microbial cultures are freeze-dried in glass ampoules and sealed with flame under a vacuum. This format is suitable for long-term preservation cultures, making it easier for researchers to use the same batch of strains and reducing genetic variation.

Example of sample tube

  • Capped Vials

Another format is to freeze-dry the cultures in small penicillin bottles with a crimped foil that holds the rubber seal in place. This is easier to open than a glass ampoule, however, in such vials the shelf life of the culture is shorter and is suitable for the storage of microorganisms used for a short period. Such as animal gavage experiments and other related microbial research.

Customers can specify the required number of ampoules or capped vials, strain identification number, and CFU specifications according to the research requirements, we can fully customize production, flexible to meet the needs of customers.


  • Low cost of maintenance.
  • Ease of transportation.


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Creative Biolabs' more than 10 years of CRO service experience provides you with the confidence to find the right partner. Services tailored to your needs provide an extension to your lab and accelerate your research projects. If you need small-scale customized production services, please do not hesitate to contact us, we can fully provide customized solutions according to your project research.

For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.

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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.

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