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Probiotic-derived Extracellular Vesicle (Exosome) Lab-Scale Production Service

We at Creative Biolabs offer end-to-end services for probiotic EV production and characterization. Our team's deep-rooted experience allows us to tailor solutions for a global clientele, supporting a spectrum of research pursuits, spanning fundamental investigations to preclinical assessments. Request a quote

Our Full Range of Probiotic-derived EVs (Exosomes) Services

Workflow

Fig.1 Various forms of extracellular vesicles. (Creative Biolabs AI)

Service Details

Probiotic Cultivation and Fermentation
Isolation of EVs
Characterization of EVs
Quality Control
Additional Services

Probiotic Cultivation and Fermentation Service

Isolation of EVs (Exosomes)

  • Harvesting of culture supernatants to separate EVs from probiotic cells.
  • Application of isolation techniques such as ultracentrifugation.

Characterization of EVs (Exosomes)

  • Physical characterization, including size, shape, and concentration.
  • Biochemical analysis to identify proteins, lipids, RNA, and other bioactive molecules within the EVs.
  • Functional assays to evaluate the biological activity of EVs, such as their immunomodulatory or antimicrobial properties.
Items Methods
Size and Morphology Analysis Nanoparticle tracking analysis (NTA)
Transmission electron microscopy (TEM)
Protein Analysis Western blotting (WB)
Mass spectrometry
Enzyme-linked immunosorbent assay (ELISA)
Metaproteomics
Lipid Analysis Lipidomics
Nucleic Acid Analysis RNA sequencing
PCR
Functional assays In vitro cell culture assays to assess biological activity.
In vivo studies to evaluate efficacy.

Quality Control (Purity and Sterility Testing)

  • Ensuring the purity and consistency of EV preparations.
  • Testing for contaminants such as endotoxins or residual probiotic cells.

Additional Services

  • Custom EV Production: Tailoring EV production for specific research applications, such as drug delivery, therapeutic interventions, or functional food development.
  • Stability Studies: Evaluating the stability of EVs under different storage conditions.

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Deliverables

They may vary depending on the client's specific needs and the stage of their research or development.

Items Contents Significance
High-Quality Probiotic EVs
  • Pure, contaminant-free EVs isolated from probiotic cultures.
This deliverable is essential for downstream applications, including in vitro and in vivo studies.
Comprehensive Characterization Data
  • Detailed analysis of EV size, concentration, and morphology using advanced tools like nanoparticle tracking analysis (NTA) or electron microscopy.
  • Molecular profiling to identify key bioactive components (proteins, lipids, RNA).
Provides a thorough understanding of the EV's properties and potential bioactivity.
Optimized Production and Formulation (if applicable)
  • Optimized Fermentation Protocols
  • Stable EV Formulations
This is essential for clients aiming for large-scale production and for any future therapeutic application.
Functional and Biological Activity Data
In Vitro Assay Results Data from cell culture assays demonstrating the effects of probiotic EVs on specific biological processes (e.g., immunomodulation, barrier function enhancement, antimicrobial activity). This provides evidence of the EVs' potential therapeutic or functional effects.
In Vivo Study Results Data from animal studies evaluating the efficacy and safety of probiotic EVs in relevant disease models. This is a crucial step for translational research and clinical applications.
Custom Functional Assay Reports Data from assays that are specifically designed around the client's needs.

Applications of EVs (Exosomes)

EVs—exosomes, microvesicles, and apoptotic bodies—mediate intercellular signaling via cargo transfer: proteins, lipids, and nucleic acids. That's the core of it.

Biological Functions

  • Probiotic EVs contribute to maintaining gut health by modulating the immune system and enhancing the intestinal barrier.
  • They exhibit antimicrobial properties, helping to inhibit the growth of harmful pathogens.
  • These vesicles can influence host cell signaling pathways, promoting anti-inflammatory and antioxidant effects.

Therapeutic Potential

  • Probiotic EVs are being explored as potential drug delivery systems due to their natural origin and biocompatibility.
  • They hold promise in treating various diseases, including gastrointestinal disorders and infections.

Research Applications

  • Studies are investigating their role in preventing or alleviating diseases through mechanisms like immune modulation and pathogen inhibition.
  • They are also being considered for use in functional foods and pharmaceuticals.

Fig.2. The colony on the plate. (Creative Biolabs AI)

Why Us?

  • Customizable Solutions: We tailor our services to meet the specific needs of each client, offering flexibility in probiotic strain selection, isolation methods, and EV formulation.
  • Comprehensive Characterization: We provide detailed characterization of the isolated EVs, giving researchers confidence in the quality and reproducibility of their experiments.
  • Expert Consultation and Support: Our team of experienced scientists provides expert consultation and ongoing support throughout the project.
  • Accelerated Research: By outsourcing EV production to Creative Biolabs, researchers can save valuable time and resources, accelerating their research progress.
  • Reproducibility: Our standardized protocols ensure batch-to-batch consistency, a critical factor for reliable research and clinical translation, supported by Published Data.

Partner with Us for Innovative Probiotic EV Solutions

Application-ready pEVs, tailored to your project. Creative Biolabs delivers the quality and innovation you need. Let's discuss how we can accelerate your research or development. Contact us.

EVs (Exosomes) Services FAQs

Why are probiotic EVs important in health and research?

Probiotic extracellular vesicles (EVs) present a cell-free platform for therapeutic delivery. Their immunomodulatory, barrier-enhancing, and antimicrobial activities suggest a potential for targeted disease interventions.

Can you optimize probiotic fermentation to maximize EV yield?

We focus on the optimization of fermentation parameters—media composition, pH, temperature, and aeration—to maximize probiotic extracellular vesicle (EV) yield.

What are the sample volume and preparation requirements?

Sample requirements vary depending on the probiotic strain and application. We provide detailed guidelines upon project initiation.

What is the typical turnaround time for Probiotic EV services?

Project timelines are contingent upon the scope and intricacy of the experimental design. We provide estimated timelines during project planning.

How is pricing determined for Probiotic EV services?

Pricing depends on factors such as probiotic strain, culture optimization, isolation method, characterization requirements, and functional assays. We provide customized quotes.

What sets your probiotic EV/exosome services apart?

We deliver integrated solutions encompassing optimized workflows, specialized consultation, and bespoke strategies designed to address client challenges, fostering both efficiency and high-caliber outcomes.

How can I get started?

Contact us to detail your requirements, and we'll provide comprehensive guidance throughout the project lifecycle.

Customer Reviews

"Using Creative Biolabs' EV production service in our research has significantly improved the consistency and yield of our EV isolations. This has greatly enhanced the reproducibility of our downstream experiments."

[07/2022], J***es

"Creative Biolabs' detailed characterization of the EVs provided us with valuable insights into their properties, allowing us to better understand their biological activity. The comprehensive report was very helpful."

[03/2024], M***a

"The ability to customize the EV formulation to our specific needs was a major advantage. Creative Biolabs' service saved us significant time and resources, allowing us to focus on other aspects of our research."

[01/2025], A***n

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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.

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