Next-Generation Probiotics Development

Get Expert Microbiome Solutions for Next-Generation Probiotics Development!

Next-Generation Probiotics (NGPs) are advanced microbial strains designed using cutting-edge biotechnology. Tailored for specific therapeutic purposes like gut health, immune modulation, and metabolic regulation, NGPs offer more precise microbiome support compared to traditional probiotics. Creative Biolabs applies its microbiota and microbiome expertise to offer process development and optimization services for NGPs.

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Fig. 1 Live biotherapeutics brochure banner (Creative Biolabs Original)

Live-Biotherapeutics - Service Brochure by Creative Biolabs

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Advanced NGPs Solutions Trusted by Industry Leaders

Partner with the industry leader and leverage our Next-Generation Probiotics Development Services to create innovative, effective solutions for the future of health.

Fig. 2 NGPs development services partner-1 (Creative Biolabs Authorized)

Fig. 3 NGPs services partner-2 (Creative Biolabs Authorized)

Fig. 4 NGPs development services partner-3 (Creative Biolabs Authorized)

Fig. 5 NGPs development services partner-4 (Creative Biolabs Authorized)

Fig. 6 NGPs development services partner-5 (Creative Biolabs Authorized)

Why NGPs Outperform Traditional Probiotics

NGPs are transforming the probiotics industry by addressing the limitations of traditional probiotics. Below is a comparison table highlighting their differences.

Feature	Traditional Probiotics	Next-Generation Probiotics (NGPs)
Strain Variety	General, broad-spectrum strains	Highly specialized and optimized strains
Targeted Action	General gut support	Disease-specific action (e.g., IBS, diabetes)
Efficacy in Irritable Bowel Syndrome (IBS)	Moderate (15-30% success)	High (50-60% success)
Clinical Trial Success Rate	~40%	>60%
Microbiome Impact	Broad, non-specific	Targeted microbiome modulation

Mainly NGP Candidates Developed by Creative Biolabs

Akkermansia muciniphlla

Atopbium vaginae

Bacteroides spp.

Bifidobacterium spp.

Parabacteroides spp.

Christensenella minuta

Clostridium spp.

Eggerthellaceae Family

Enterococcus spp.

Escherichia coli

Faecalibacterium prausnitzii

Pedioccus pentosaceus

Lactobacillus spp.

Prevotella copri

Salmonelle

Streptococcus spp.

Eubacterium hallii

Oxalobacter formigenes

Blautia spp.

Ruminococcus spp.

Microbiome Analysis and Qualification for NGPs

Before an NGP candidate is approved for clinical or commercial use, rigorous microbiome analysis and qualification are essential. These allow for the identification of optimal microbial strains and their interaction with the existing microbiome. Creative Biolabs offers several essential services to ensure the optimal development and validation of NGPs.

Microbial Isolation and Screening

Identifying and isolating specific strains that show potential for therapeutic applications.

Microbial Identification

Using advanced techniques to accurately identify and characterize microbial strains, crucial for ensuring strain purity and consistency.

Host-Microbe Interaction Tests

Evaluating how probiotic strains interact with the human microbiome and their impact on gut health and immunity.

Antimicrobial Susceptibility Testing

Assessing the resistance of NGP candidates to various antimicrobial agents, ensuring their efficacy in different environments.

Stress Response Profiling

Analyzing how NGP strains respond to different environmental stressors, which is key to selecting robust strains that can survive in the gastrointestinal tract.

Biological Safety Test

Ensuring the safety of NGP candidates by assessing microbial species, vitality, virulence, contamination risks, and host interactions.

NGPs Development Services Process

Fig. 7 NGPs development services technology-1 (Creative Biolabs Authorized)

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Advanced Technologies for NGPs Development

Design of Engineered Therapeutic Strains

The core of NGP development lies in the design and creation of engineered therapeutic strains. By utilizing genetic modification techniques, Creative Biolabs can tailor probiotics to express therapeutic proteins or target specific diseases. For example, the engineered Lactobacillus and engineered Lactococcus lactis strains can be engineered to express proteins such as human FGF21 or proteins secreted by Akkermansia muciniphila, both of which have shown promise in modulating metabolism and enhancing gut health.

Gut Microbiota Research Approaches

Understanding the complex interactions between NGPs and the gut microbiota is a fundamental step in ensuring their efficacy. Creative Biolabs employs a wide range of gut microbiota research techniques to evaluate how different strains influence the microbiome. Technologies such as 16S rRNA sequencing, metagenomics, and metatranscriptomics allow for comprehensive profiling of the gut microbiota, ensuring that the engineered probiotics not only survive but thrive and positively interact with existing microbiota.

In Vitro and In Vivo Models for NGP Assessment

To thoroughly evaluate the therapeutic potential of NGPs, Creative Biolabs utilizes advanced in vitro and in vivo models. These models provide essential insights into how NGPs interact with the gut barrier and influence host-microbial interactions. For instance, organoid technology and microfluidic cell cultures are used to simulate gut conditions, while germ-free mouse models allow for the study of bacterial colonization and the effects of probiotics in a controlled, pathogen-free environment.

Microbial Identification and Safety Assessment

Ensuring the safety of NGPs is paramount. Creative Biolabs utilizes a variety of microbial identification methods to assess the strain's purity and safety. Techniques such as 16S rRNA gene, MALDI-TOF MS, and whole-genome sequencing (WGS) are used to ensure the strains are correctly identified and free from harmful contaminants. Furthermore, antibiotic susceptibility testing methods, including the epsilometer test (Etest) and disk diffusion, are employed to confirm that the probiotics do not harbor resistance to commonly used antibiotics, thus safeguarding against any unintended risks.

Sample Submission

If you are ready to submit your samples for testing or development, please fill out the Sample Submission Form.

Sample Name for Report/Description

Unique Identifier for Report (Lot, Run#, etc.)

Source of material(s)/Manufacturer(s)

Sample mass

Sample state (Liquid, solid, or other)

Others

Fig. 8 Sample submission form (Creative Biolabs Original)

Featured Case Studies of Next-Generation Probiotic Development

Case #1
Case #2

Innovative Probiotic Development with Bacteroides vulgatus

Creative Biolabs successfully developed B. vulgatus lyophilized powder, which has been used to study its potential therapeutic effects in animal models. By preparing the product with high-quality standards, including precise bacterial concentrations and contamination controls, the product has shown to meet research needs, such as studying the alleviation of metabolic diseases in mice.

Fig. 9 B. vulgatus strain preparation (Creative Biolabs Original)

Fig.1 B. vulgatus strain preparation. (a) Gram staining of B. vulgatus strain. (b) The colonies of B. vulgatus on Columbia Blood Agar

Case Study

Innovative Probiotic Development with Bacteroides vulgatus

Download this case study to discover the full process behind the preparation of B. vulgatus lyophilized powder, including its manufacturing, applications in disease research, and therapeutic benefits.

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Why Choose Creative Biolabs for NGP Solutions?

Our unique combination of integrated technologies, customized solutions, expert team, and comprehensive support provides unparalleled structural biology insights, accelerating your research from basic characterization to applications.

Cutting-Edge Technology and Expertise

We employ state-of-the-art platforms like Synchronized Lysis Circuit (SLC) Technology, recombinant strain engineering, and advanced microbiome analysis to ensure the development of highly specialized and effective NGPs. Our team of experts is dedicated to leveraging the latest scientific advancements to create tailored probiotic solutions that meet the specific needs of each client.

Comprehensive Service Offering

From strain selection and formulation optimization to clinical testing and regulatory support, Creative Biolabs provides a full spectrum of NGP development services. This end-to-end service ensures a streamlined development process, saving you time and resources while guaranteeing that all aspects of the NGP lifecycle are covered.

Customization for Specific Research Needs

At Creative Biolabs, we understand that each project is unique. Our ability to tailor probiotic strains to address specific research needs, whether related to gut health, immune function, or metabolic disorders, sets us apart. We work closely with clients to design NGPs that provide targeted, personalized solutions.

Commitment to Safety and Quality

We adhere to the highest standards of safety and quality throughout the development process. Our microbial identification and safety assessment procedures, including antibiotic susceptibility testing, ensure that all NGPs meet strict safety requirements, minimizing risks and maximizing therapeutic benefits.

Fig. 15 NGPs development services advantages (Creative Biolabs Authorized)

What Our Customers Say

"Partnering with Creative Biolabs for our Next-Generation Probiotics development has been a game-changer. Their expertise in microbiome analysis and engineered strain development allowed us to create a highly specialized probiotic solution for our research on IBS treatment. The tailored approach and cutting-edge technologies like CRISPR/Cas9 have significantly accelerated our timeline. The team's attention to detail and commitment to safety and quality were evident throughout the entire process. We are excited to see the results in clinical trials!"

Dr. Emily Tran Director of Microbial Research at a biotech firm

"We were impressed by Creative Biolabs' deep understanding of microbiota interactions and their ability to design probiotics with specific therapeutic actions. Their work on engineered Lactobacillus strains for metabolic health was instrumental in advancing our research. The high success rate in pre-clinical trials, combined with their advanced safety assessment protocols, gave us confidence that we were working with the best in the industry."

John Mitchell Senior R&D Scientist at a therapeutics company

"Creative Biolabs exceeded our expectations with their Next-Generation Probiotics services. Their ability to tailor probiotics to our specific needs in gut health has allowed us to create more targeted therapies for our patients. From microbial identification to safety testing and clinical trial preparation, every step was handled professionally. The expertise they brought in designing engineered therapeutic strains made a significant difference in our product's effectiveness."

Sophia Lee Lead Product Development Manager at a leading biotech company

FAQs About Our NGPs Development Services

How do you ensure the genetic stability of engineered probiotic strains over multiple generations?

Creative Biolabs employs extensive genomic monitoring, stability testing across multiple passages, and stress condition exposure to verify that genetic modifications remain stable throughout production cycles.

How do you determine the optimal delivery system for different NGP strains?

We evaluate various formulation options based on strain characteristics, target site, and stability requirements, including microencapsulation and controlled-release technologies.

What measures ensure batch-to-batch consistency in NGP production?

We implement rigorous quality control protocols, standardized production processes, and advanced analytical methods to maintain consistent product quality.