Bacteroides uniformis was isolated from human feces.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
CAT
Size
Price
Quantity
LBST-033FG
Product Information
Product Overview
Bacteroides uniformis was isolated from human feces.
Target
Bacteroides
Genus
Bacteroides
Application
Study and research
Type Strain
Yes
Culture Medium
PYG
Culture Conditions
37°C; Anaerobic
Source
Human feces
Risk Group
2
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Bacteroides is a group of promising candidates that has attracted major attentions from scientists as model organisms or community for the investigations of the intestinal microbiota field, due to their powerful adaption characteristics in the host and especially the underlying benefits. Bacteroides is a genus of Gram-negative, obligate anaerobic bacteria. Bacteroides species are non endospore-forming bacilli, and may be either motile or nonmotile, depending on the species. Bacteroides; order is the most abundant group of Gram-negative bacteria which flourish in the human intestine at high densities of 109-1011 CFU per gram of feces, and constitutes over 30 species which are more closely related than members in other bacteria orders. And, Bacteroides is considered as one of the major genera of the core microbiota module, of which species with relative abundance of over 1% refers to B. uniformis, B. vulgatus, B. caccae, and B. thetaiotaomicron. In addition to the unique physiological characteristics and the special ecological dynamics, Bacteroides species are under investigations for their underlying beneficial dialogues with the host. Bacteroides species to be considered as next-generation probiotics, and the application of which in prevention or intervention in dysbiosis of gut microbiota and the associated disorders requires further investigations in the modulatory mechanisms and safety evaluation. Bacteroides is one of the dominant contributors of SCFA among the intestinal commensals, mostly in the form of acetate and propionate. Accumulated acetate blocks the transportation of toxins between gut lumen and blood, while propionate is capable of inducing the apoptosis of human colon carcinoma cells, and thus avoids the formation of tumors. Moreover, Vitamin K, which is mainly synthesized by gut microbiota candidates such as Bacteroides, can help prevent or treat osteoporosis by increasing bone mineral density.
Alternative Names
Bacteroides uniformis
FAQsPublished Data Customer Reviews Related Products
How should Bacteroides uniformis be revived from a frozen state?
To revive Bacteroides uniformis, thaw the frozen stock quickly at room temperature and immediately inoculate into pre-reduced, anaerobic growth medium. Incubate under strict anaerobic conditions at 37°C to ensure optimal recovery and growth.
What type of medium is recommended for the routine cultivation of Bacteroides uniformis?
Bacteroides uniformis grows well in Brain Heart Infusion (BHI) broth supplemented with yeast extract, hemin, and vitamin K1. This medium supports its complex nutritional requirements and promotes robust growth under anaerobic conditions.
How can the viability and purity of Bacteroides uniformis cultures be confirmed?
Viability can be assessed by plating on anaerobic agar plates and checking for colony growth. Purity can be verified using Gram staining and PCR amplification of specific 16S rRNA gene sequences to confirm the absence of contaminants.
Can Bacteroides uniformis be used in co-culture experiments with other gut microbiota?
Yes, Bacteroides uniformis can be used in co-culture experiments to study its interactions with other gut microbes. Ensure that the growth conditions support all species involved and monitor interactions using techniques like fluorescence in situ hybridization (FISH) or metagenomic sequencing.
How should genetic modifications be approached with Bacteroides uniformis?
Genetic modifications in Bacteroides uniformis can be performed using electroporation or conjugation methods to introduce DNA. It's important to select appropriate antibiotic resistance markers for selection and to confirm integration using PCR and sequencing techniques.
B. uniformis CECT 7771 combined with WBE reduces weight gain in mice fed HFHSD
The research focuses on the significant therapeutic potential of Bacteroides uniformis in treating obesity through modulating gut microbiota. It highlights the combined effect of Bacteroides uniformis CECT 7771 and wheat bran extract (WBE), a fiber source, in diet-induced obese mice. The study demonstrates that this combination more effectively reduces body weight and improves metabolic health compared to each treatment alone. Specifically, the mixture reduced weight gain, enhanced insulin sensitivity, and regulated lipid metabolism by impacting metabolic pathways in the liver and adipose tissue. Experimental data indicated that Bacteroides uniformis, particularly when combined with WBE, increased the production of short-chain fatty acids (SCFAs), including butyrate, known for their beneficial effects on energy metabolism and inflammation reduction. The combination treatment also significantly restored levels of intraepithelial lymphocytes and type-3 innate lymphoid cells in the gut, enhancing the immune barrier against diet-induced physiological disturbances. Collectively, these findings underline the ability of targeted dietary interventions to rectify metabolic dysfunctions by manipulating gut microbiota, presenting a promising strategy for managing obesity and associated metabolic disorders.
López-Almela, I., Romaní-Pérez, M., et al. Bacteroides uniformis combined with fiber amplifies metabolic and immune benefits in obese mice. Gut microbes. 2021, 13(1): 1-20.
Reliable Research Partner
The reliability and high purity of Bacteroides uniformis from Creative Biolabs have significantly enhanced our microbiota-related studies, showing clear advantages over other products.
Precision in Metabolic Studies
Creative Biolabs' Bacteroides uniformis has been instrumental in our metabolic research, offering precise insights into gut flora's role in obesity and diabetes.
Unmatched in Gut Microbiome Research
The Bacteroides uniformis from Creative Biolabs provides unmatched quality, making it a top choice for our studies on the gut-brain axis and its therapeutic potentials.
Breakthrough in Gastrointestinal Health Bacteroides uniformis from Creative Biolabs has propelled our research into new realms of gastrointestinal health, thanks to its exceptional adaptability and robust growth.
A Foundation for Microbial Interactions
Using Bacteroides uniformis from Creative Biolabs has laid a strong foundation for our studies on microbial interactions, proving superior to other strains with its consistent performance.
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