Bacteroides thetaiotaomicron was isolated from human feces. It is a Gram-negative obligate anaerobe.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
CAT | Size | Price | Quantity | |
---|---|---|---|---|
LBST-031FG |
|
Product Information | |
---|---|
Product Overview | Bacteroides thetaiotaomicron was isolated from human feces. It is a Gram-negative obligate anaerobe. |
Target | Bacteroides |
Genus | Bacteroides |
Application | Study and research |
Type Strain | Yes |
Culture Medium | Columbia blood agar |
Culture Conditions | 37°C; Anaerobic |
Source | Human feces |
Risk Group | 2 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
---|---|
Introduction | Bacteroides is a group of promising candidates that has attracted major attentions from scientists as model organisms or community for the investigations of the intestinal microbiota field, due to their powerful adaption characteristics in the host and especially the underlying benefits. Bacteroides is a genus of Gram-negative, obligate anaerobic bacteria. Bacteroides species are non endospore-forming bacilli, and may be either motile or nonmotile, depending on the species. Bacteroides; order is the most abundant group of Gram-negative bacteria which flourish in the human intestine at high densities of 109-1011 CFU per gram of feces, and constitutes over 30 species which are more closely related than members in other bacteria orders. And, Bacteroides is considered as one of the major genera of the core microbiota module, of which species with relative abundance of over 1% refers to B. uniformis, B. vulgatus, B. caccae, and B. thetaiotaomicron. In addition to the unique physiological characteristics and the special ecological dynamics, Bacteroides species are under investigations for their underlying beneficial dialogues with the host. Bacteroides species to be considered as next-generation probiotics, and the application of which in prevention or intervention in dysbiosis of gut microbiota and the associated disorders requires further investigations in the modulatory mechanisms and safety evaluation. Bacteroides is one of the dominant contributors of SCFA among the intestinal commensals, mostly in the form of acetate and propionate. Accumulated acetate blocks the transportation of toxins between gut lumen and blood, while propionate is capable of inducing the apoptosis of human colon carcinoma cells, and thus avoids the formation of tumors. Moreover, Vitamin K, which is mainly synthesized by gut microbiota candidates such as Bacteroides, can help prevent or treat osteoporosis by increasing bone mineral density. |
Alternative Names | Bacteroides thetaiotaomicron |
Bacteroides thetaiotaomicron interacts with the host's immune system in several ways, including the modulation of immune responses through mechanisms like the production of regulatory dendritic cell responses via its outer membrane vesicles. This interaction is vital for maintaining intestinal homeostasis and can influence immune tolerance.
For optimal growth, Bacteroides thetaiotaomicron should be cultured under strict anaerobic conditions at 37°C. It thrives in media that simulate the nutrient-rich environment of the gut, often requiring complex growth supplements including blood or hemin.
Growth can be monitored by measuring optical density at 600 nm, while metabolic activity may be assessed through assays that quantify the production of short-chain fatty acids or other metabolic products. Advanced techniques like gas chromatography may be used for detailed metabolic profiling.
Yes, genetic modification techniques are available for Bacteroides thetaiotaomicron. These include methods like CRISPR-Cas9 and other gene editing tools, which can be employed to study gene function or to create strains with desirable traits for further research.
Yes, Bacteroides thetaiotaomicron is valuable in biotechnological research due to its capabilities in polysaccharide breakdown, making it a model organism for studying gut microbiota interactions and the development of microbial therapeutics aimed at treating digestive diseases.
B. thetaiotaomicron is protective in an IL10KO mouse model of colitis
The study details the efficacy of Bacteroides thetaiotaomicron in alleviating symptoms of Crohn's disease through preclinical models. The study highlights the bacterium's protective properties in various models of inflammatory bowel disease (IBD), particularly focusing on its anti-inflammatory impacts. It was observed that Bacteroides thetaiotaomicron significantly reduced weight loss, colon shortening, and histopathological damage in both dextran sodium sulfate (DSS) and interleukin-10 knockout (IL10KO) rodent models of colitis.
One of the key experimental findings involved the bacterium's ability to modulate immune responses, as demonstrated by a significant reduction in pro-inflammatory markers in treated animals. This indicates that the bacterium not only acts through its presence but possibly through the secretion of active biomolecules like the Pirin-like protein, which was shown to reduce NF-κB signaling, a pathway often linked with inflammation. These findings underscore the potential of Bacteroides thetaiotaomicron to serve as a novel therapeutic alternative to traditional treatments for Crohn's disease, which often involve extensive use of immunosuppressants and biologics with considerable side effects.
Delday, M., Mulder, I., et al. Bacteroides thetaiotaomicron ameliorates colon inflammation in preclinical models of Crohn's disease. Inflammatory bowel diseases. 2019, 25(1): 85-96.
Click the button below to contact us or submit your feedback about this product.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Copyright © 2024 Creative Biolabs. All Rights Reserved.