Freeze-dried Lactobacillus acidophilus Powder
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information | |
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Product Overview | Freeze-dried Lactobacillus acidophilus Powder |
Target | Lactobacillus |
Source | Human |
CFU Value | 2×10^11 |
Genetic Modifications | None |
Application | Used as live bacteria probiotic |
Appearance | White to light yellow powder |
Size | 1 kg |
Storage | Store at -20°C to -80°C. |
Shelf Life | 24 months |
Note | 1. Characters of the strain are genetically identified. 2. Types of probiotic blends/premixes could be provided based on the clients' requirements. 3. Tyndalized (Heat-Sterilized) specifications could be provided based on clients' requirements. 4. Complete data sheet is available based on the clients' requirements. 5. For research use only. Not intended for food, diagnostics, therapeutic or any in vivo human use. |
Target Introduction | |
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Introduction | Lactobacillus acidophilus is a species of gram-positive bacteria in the genus Lactobacillus. L. acidophilus is a homofermentative, microaerophilic species, fermenting sugars into lactic acid, and grows readily at rather low pH values (below pH 5.0) and has an optimum growth temperature of around 37 °C (99 °F). L. acidophilus is found in the human and animal gastrointestinal tract and mouth. Some strains of L. acidophilus may be considered to have probiotic characteristics. |
Alternative Names | Lactobacillus acidophilus; L. acidophilus |
Lactobacillus acidophilus powder should be rehydrated in sterile deionized water or an appropriate buffer solution. Gently swirl to mix and avoid vigorous shaking to maintain cell integrity. This ensures a viable and homogenous bacterial suspension.
During lyophilization, factors such as temperature control, vacuum levels, and the use of protective cryoprotectants are crucial. These factors help preserve the viability and functionality of Lactobacillus acidophilus for long-term storage.
For gastrointestinal simulation studies, the powder should be reconstituted and added to simulated gastric or intestinal fluids under controlled conditions. This process helps study the survival and activity of the bacteria through different digestive phases.
When co-culturing with human cell lines, a concentration of 10^6 to 10^8 CFU/mL of Lactobacillus acidophilus is typically used. This concentration is sufficient to study interactions and effects on cellular responses.
Genetic manipulation of Lactobacillus acidophilus involves techniques such as electroporation or conjugation. Proper preparation of competent cells and the use of specific plasmids or vectors are essential for successful genetic transformation.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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