Bifidobacterium longum was isolated from microbial inoculum. It is a Gram-positive, catalase-negative, rod-shaped bacterium. It is a microaerotolerant anaerobe and considered to be one of the earliest colonizers of the gastrointestinal tract of infants.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
CAT
Size
Price
Quantity
LBST-056FG
Product Information
Product Overview
Bifidobacterium longum was isolated from microbial inoculum. It is a Gram-positive, catalase-negative, rod-shaped bacterium. It is a microaerotolerant anaerobe and considered to be one of the earliest colonizers of the gastrointestinal tract of infants.
Target
Bifidobacterium
Genus
Bifidobacterium
Strain Designation
BB536
Application
Study and research
Type Strain
No
Culture Medium
BBL
Culture Conditions
37°C; Anaerobic
Source
Microbial inoculum
Characteristics
The thallus is rod-shaped, with Y-shaped bifurcation and Gram-positive.
Risk Group
1
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Bifidobacterium are Gram-positive, heterofermentative, anaerobic bacteria with a distinctive bifid; i.e. Y shape after which they are named. They are originally isolated from the faeces of breast-fed infants, members of the genus Bifidobacterium are commonly found in the GI tract of mammals. In humans, Bifidobacterium resides within the GI tract, from birth to old age. Disturbances in the microbiota are linked to an ever-growing number of immune-linked disease states including IBD, atopic allergy, arthritis, and obesity. Therefore, there is a significant interest in treating these diseases through microbial or "probiotic" supplementation of patients, including Bifidobacterium. Data from mouse models and clinical trials indicate that Bifidobacterium may have beneficial effects for treating and preventing immune-linked diseases, including gut-associated and systemic conditions. Bifidobacterium have been commercially exploited as probiotic agents due to their associated health benefits and GRAS; Generally Recognised As Safe status.
Alternative Names
Bifidobacterium longum
FAQs Customer Reviews Related Products
What is the recommended protocol for culturing Bifidobacterium longum?
To culture Bifidobacterium longum, begin by rehydrating the freeze-dried powder in an anaerobic medium suitable for bifidobacteria, such as MRS broth supplemented with cysteine. Incubate at 37°C under anaerobic conditions for 24-48 hours. Monitor growth using optical density measurements to ensure optimal bacterial proliferation.
How should Bifidobacterium longum be prepared for animal studies?
For animal studies, Bifidobacterium longum should be prepared by suspending the bacterial culture in a suitable vehicle, such as sterile saline or phosphate-buffered saline (PBS). Ensure the concentration is standardized, typically around 10^9 CFU/mL. Administer orally via gavage or mix into the animal's diet or drinking water.
What are the critical steps for ensuring the viability of Bifidobacterium longum during experiments?
Ensuring the viability of Bifidobacterium longum involves maintaining anaerobic conditions, using appropriate growth media, and avoiding repeated freeze-thaw cycles. When preparing inoculums, handle the bacteria gently and keep them at low temperatures to preserve their viability until use.
How can the effectiveness of Bifidobacterium longum be assessed in a research setting?
The effectiveness of Bifidobacterium longum can be assessed through various assays, including monitoring changes in gut microbiota composition, measuring immune response markers, and evaluating physiological effects in animal models. Techniques such as qPCR, flow cytometry, and metabolomic analysis are commonly used.
What are the common challenges in working with Bifidobacterium longum and how can they be mitigated?
Common challenges include maintaining anaerobic conditions, ensuring consistent bacterial counts, and preventing contamination. These can be mitigated by using anaerobic chambers or gas packs, performing regular viability checks, and employing stringent aseptic techniques. Regular training and adherence to protocols are also crucial.
Exceptional Quality for Microbiome Research Bifidobacterium longum from Creative Biolabs has significantly enhanced microbiome research. Its consistent viability and purity are unparalleled, ensuring reliable results.
Reliable and Consistent Results
The strain provides reliable and consistent results in inflammation studies. Its stability and effectiveness outperform other similar products, making it a valuable addition to any research.
Superior Strain for Immune Modulation
Creative Biolabs' Bifidobacterium longum is superior for immune modulation studies. The high viability and reproducibility of results have greatly benefited our experimental outcomes.
High Viability and Purity
The viability and purity of Bifidobacterium longum are excellent. This strain has proven effective in various gut health studies, providing reproducible and accurate data every time.
Best Choice for Probiotic Research
The strain is the best choice for probiotic research. Its effectiveness in modulating gut microbiota and immune response is outstanding, making it indispensable for our studies.
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