Streptococcus oralis subsp. dentisani is a microaerophile, mesophilic, Gram-positive human pathogen that was isolated from human caries-free tooth surface.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information | |
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Product Overview | Streptococcus oralis subsp. dentisani is a microaerophile, mesophilic, Gram-positive human pathogen that was isolated from human caries-free tooth surface. |
Target | Streptococcus |
Genus | Streptococcus |
Strain Designation | 7746, Str. 7746 |
Application | Study and research |
Culture Medium | Columbia blood agar |
Culture Conditions | 37°C; Microaerophilic |
Source | Human caries-free tooth surface |
Risk Group | 1 |
Genbank Accession Numbers | 16S rRNA gene: HG315102 23S rRNA gene: HG315104 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
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Introduction | Streptococcus is a genus of Gram-positive coccus that belongs to the family Streptococcaceae, within the order Lactobacillales. Streptococcus thermophilus is a powerful probiotic strain that has well researched health benefits. This probiotic is often found in the colon and has many digestive, immunity and many other researched health benefits. |
Alternative Names | Streptococcus oralis subsp. dentisani; 7746 |
Streptococcus oralis subsp. dentisani plays a crucial role in microbiome studies, particularly in oral health research. It is known for its ability to inhibit cariogenic bacteria, making it a target for probiotic development. Researchers often use this strain to explore its antimicrobial properties and its interaction with the oral microbiome, which can lead to innovative therapeutic strategies for oral diseases.
For optimal growth in laboratory settings, Streptococcus oralis subsp. dentisani requires cultivation in a medium rich in nutrients that mimic the human oral cavity environment. Commonly used media include trypticase soy agar supplemented with 5% defibrinated sheep blood. Researchers should maintain anaerobic or microaerophilic conditions to support its growth, and incubation typically occurs at 37°C for 24 to 48 hours.
Researchers often assess the antimicrobial properties of Streptococcus oralis subsp. dentisani through in vitro assays, such as co-culture experiments with pathogenic bacteria like Streptococcus mutans. These assays can include agar diffusion tests or biofilm inhibition studies. The strain's ability to produce bacteriocins and other antimicrobial peptides is also explored using genomic and proteomic techniques.
When investigating the probiotic potential of Streptococcus oralis subsp. dentisani in animal models, researchers commonly use rodent models of oral disease or gut microbiota studies. The strain is introduced orally or via a gavage method, and its effects on microbial balance, immune modulation, and pathogenic inhibition are monitored. Care must be taken to ensure the survival of the bacteria through the gastrointestinal tract.
The genome of Streptococcus oralis subsp. dentisani can be sequenced using next-generation sequencing technologies. Researchers can utilize bioinformatics tools to analyze its genetic makeup, focusing on gene clusters responsible for bacteriocin production, carbohydrate metabolism, and adherence factors. Comparative genomic studies may also reveal unique traits that contribute to its probiotic potential.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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