Creative Biolabs performs targeted isolation of L. reuteri from fecal, food, or fermented samples. Candidate strains are subjected to both primary and secondary screening according to critical criteria including acid and bile salt tolerance, epithelial and mucus adhesion properties, and metabolite production such as reuterin and exopolysaccharides (EPS). This tiered strategy ensures that only robust, functionally relevant isolates progress into downstream workflows.
To confirm the identity of L. reuteri, we apply 16S rRNA sequencing, whole-genome sequencing, and biochemical assays. These approaches not only verify the strain but also trace its lineage and provide comprehensive annotation of key functional genes, such as adhesins, bile salt hydrolases, and metabolic clusters. Accurate identification establishes a reliable foundation for further experimental characterization and application.
Our platform optimizes cultivation under controlled anaerobic conditions, adjusting medium composition, pH, agitation, and feeding strategies. This enables scale-up from shake flask to bioreactor with consistent performance. The process is designed to maximize viable cell yield and enhance the output of metabolites such as reuterin, ensuring seamless transition to larger-scale research and industrial feasibility testing.
To maintain strain viability during storage and transportation, we develop stabilization systems using lyophilization or spray drying. Protective excipients such as trehalose or skim milk are integrated into the process to safeguard cells from stress. Accelerated and long-term stability studies further verify that L. reuteri retains its functional and metabolic activity over time.
We design research-grade formulations that combine dried L. reuteri powders with prebiotics, buffering systems, and moisture-protection strategies. These can be prepared into capsules, tablets, sachets, or liquid suspensions. Each format is optimized for rehydration performance, survival of viable cells, and usability, making them adaptable for different application pipelines.
Comprehensive evaluation is performed to assess the functional properties of L. reuteri. Tests include antimicrobial activity against opportunistic pathogens, quantification of reuterin production, adhesion to epithelial and mucus layers, barrier function modulation, and metabolite profiling of organic acids and short-chain fatty acids. Together, these assays provide detailed insights into the strain’s mechanisms of action.
Using in vitro co-culture systems of human peripheral immune cells and epithelial cells, Creative Biolabs analyzes cytokine expression, Toll-like receptor (TLR) signaling pathways, and macrophage or dendritic cell polarization. These assays quantify the immune-modulating potential of L. reuteri and link microbial activity with host cellular responses.
Creative Biolabs has established an advanced gene-editing technology platform for L. reuteri. Our system supports precise engineering of strains for research use, enabling the modification of adhesion factors, metabolite pathways, or the integration of reporter constructs. This capability allows researchers to investigate causal mechanisms and build custom strains tailored to specific project goals.
Fig.1 Gram staining of L. reuteri.
