Ruminococcus bromii
Microbiome CRO Services

Q: What is the function of Ruminococcus bromii?

Ruminococcus bromii acts as a keystone degrader of resistant starch in the human gut, initiating carbohydrate breakdown and supporting butyrate-producing partners through efficient cross-feeding mechanisms and short-chain fatty acid generation.

Q: Can you test cross-feeding toward butyrate without overcomplicating the model?

Yes. We deploy tiered co-cultures where R. bromii is paired with typical butyrogens and controls, monitoring pH, lactate, and SCFA to prevent confounding bloom dynamics while preserving interpretability.

Creative Biolabs helps microbiome teams generate decision-grade data for Ruminococcus bromii—quantifying resistant-starch degradation, SCFA outputs, and partner synergy under rigorously controlled anaerobic conditions. Turn exploratory concepts into reproducible metrics that guide selection, formulation, and scale-up.

R. bromii microbiome CRO services banner (Creative Biolabs Original)

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Trusted by Leading R&D Teams

Global microbiome innovators choose Creative Biolabs for rigorous anaerobe handling, mechanism-centric assays, and transparent data packages that streamline internal reviews and regulatory-ready documentation.

Why Focus on R. bromii?

A growing body of research recognizes R. bromii as a keystone degrader of resistant starch (RS), initiating carbohydrate access for the wider community and enabling downstream SCFA networks, including butyrate via cross-feeding partners. These functions make R. bromii central to diet–microbiome interactions and precision prebiotic programs.

Mechanistically, R. bromii employs a membrane-anchored amylosome with specialized starch-binding proteins to adhere to and hydrolyze granular RS, releasing sugars that support proximal taxa. These traits create measurable, formulation-relevant readouts in vitro and in co-culture systems.

R. bromii starch fermentation (Creative Biolabs Original)

Service Modules Built for R. bromii Projects

Microbial Isolation and Screening Services

From fecal/anaerobic enrichment matrices, R. bromii is selectively recovered using RS-enriched media and colony phenotyping under strict anaerobiosis. We prioritize strains showing strong particle-RS adhesion and hydrolysis, reflecting amylosome-mediated activity and cross-feeding potential. All steps emphasize oxygen protection and traceability for high-value isolates.

Microbial Identification Services

Species-level resolution for R. bromii is achieved with 16S rRNA and whole-genome sequencing, complemented by ANI to discriminate near neighbors. Genomic annotation highlights starch-active loci and amylosome components to inform functional hypotheses, build a searchable strain dossier, and support long-term lineage tracking.

Specific Primers and Probes Design

We build R. bromii species/strain-level qPCR assays—validated against reference panels—to quantify abundance in fermentations, bioprocess runs, or complex consortia. Probes are aligned to conserved starch-utilization signatures where appropriate, enabling sensitive monitoring of R. bromii kinetics during process optimization and stability programs.

Carbohydrate Fermentative Profiles

Using resistant starches and select oligosaccharides as substrates, we map R. bromii fermentation fingerprints, tracking acetate yields, pH, and gas, while modeling cross-feeding to butyrate producers in co-culture. These datasets guide formula design, partner matching, and pH/lactate controls that shape SCFA outcomes.

Lab-scale Production Services

We scale R. bromii from bench (100 mL) to multi-liter under validated anaerobic controls. Each batch includes KQAs: viable counts, purity, RS-degradation potency, and metabolic fingerprints. Results steer bioreactor parameters, media selection, and harvest strategies, informing feasibility for later scale-up and formulation.

Microbial Stabilization Services

Given oxygen sensitivity, R. bromii requires tailored protection. We screen cryo-/lyoprotectants, freeze-drying or spray-drying conditions, and packaging atmospheres to maximize shelf stability and rapid revival with preserved RS-degrading activity—validated by post-recovery functional assays.

Host-Microbe Interaction Tests

We evaluate R. bromii in mucin/epithelial co-cultures, quantifying TEER, tight-junction markers, and SCFA-mediated effects. Pairing with commensal partners tests synergy driven by R. bromii RS hydrolysis and glucose halos, generating decision-grade MoA data for claim substantiation and partner selection.

Functional and MoA Screening

Mechanism-first assays center on R. bromii amylosome function—binding to granular RS, hydrolysis rates, and cross-feeding efficacy to butyrate producers. Results are tied to genetics/omics where useful, producing quantitative readouts that de-risk portfolio down-selection.

How We Work: A Clear, Modular Workflow

Anaerobic Intake & Triage

Receive/process samples under oxygen-free conditions; initiate RS-enriched pre-cultures for R. bromii recovery.

Strain Discovery & ID

Isolate, dereplicate, and confirm R. bromii via 16S/WGS and ANI; assemble annotated strain dossiers.

Mechanism-Oriented Assays

Run amylosome adhesion/hydrolysis tests and fermentation profiling; add co-culture cross-feeding as needed.

Host-Interface Modeling

Test barrier endpoints and SCFA-linked effects in relevant epithelial/mucus systems.

Process & Stability Design

Define growth, harvest, and stabilization parameters tailored to R. bromii oxygen sensitivity.

Report & Next Steps

Deliver data packages with methods, QC, and interpretation; align on scale-up or product-adjacent studies.

Advantages of Our R. bromii CRO Services

Extensive Technical Expertise

Decades of experience in microbiome and anaerobic research ensure dependable handling of delicate species like R. bromii.

Integrated Project Support

From strain isolation to data interpretation, every step is coordinated for consistency and transparency.

Flexible and Customizable Design

Services are tailored to meet unique project goals, experimental setups, and research timelines.

Quality and Reproducibility

Standardized procedures and stringent controls guarantee data accuracy and repeatability.

Collaborative Communication

Clients receive continuous updates, technical consultation, and responsive scientific support throughout the project.

Global Reliability

Trusted by academic and industrial researchers worldwide for excellence in microbiome contract research services.

What's the Applications of R. bromii?

Keystone Resistant-Starch Degrader

As the primary initiator of resistant-starch breakdown, R. bromii drives acetate/formate production, cross-feeds butyrate producers, nourishes colonocytes, and stabilizes community structure—foundational for microbiome-focused diets and consortia engineering.

Probiotic & Prebiotic Strategies

Leveraging starch degradation, R. bromii restores SCFA balance and counters dysbiosis; fiber plans should be personalized because low baseline R. bromii constrains acetate/formate yields and downstream butyrate generation.

Preclinical Liver Fibrosis Mitigation

In liver-fluke–infected mice, oral R. bromii increased acetate, activated PI3K/AKT anti-fibrotic signaling, and reinforced intestinal barrier integrity—suggesting microbiome-driven avenues to limit fibrogenesis and support hepatic resilience via acetate.

Early-Life Allergy Modulation

Infant cohorts associate lower R. bromii with higher atopic dermatitis risk; sustaining early resistant-starch degradation and cross-feeding boosts butyrate, strengthens gut maturation, and may reduce allergy trajectories later.

Exercise Adaptation Interface

Mouse studies link higher R. bromii abundance to improved running; future athlete interventions may tune resistant-starch intake and microbial consortia to enhance motivation, energy harvest, signaling, and performance adaptation capacity.

Biotechnological Leverage via Amylosomes

Surface multi-enzyme amylosomes of R. bromii model efficient granular-starch deconstruction, informing prebiotic design, food processing, consolidated bioprocessing, and synthetic consortia engineering for carbohydrate-active system innovation in industrial and academic research.

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Case Study : Lab-Scale Custom Production of R. bromii

A client engaged Creative Biolabs to generate lab-scale R. bromii lyophilized powder for preliminary evaluation in a mouse colitis model. Using strain CAT# LBSX-0522-GF79, cultures were grown anaerobically at 37 °C for 48 h in PYG broth. Pre-experiments optimized harvest conditions and lyophilization parameters, followed by activity checks to confirm functional recovery post-process.

Quality control included Gram staining and continuous microscopic examination to verify purity (Gram-positive, no contamination), plus viability and activity verification after drying. The final deliverable was a white to light-yellow lyophilized powder (1 g/bag; 5 bags total) in foil packaging, recommended for storage at ≤4 °C with a documented one-year shelf life—ready for downstream RUO studies.

Gram staining of Ruminococcus bromii (Creative Biolabs Original)

Fig.1 Gram staining of R. bromii. (Microscopic examination)

Custom Lab-scale Production of 3 Probiotics Brochure (Creative Biolabs Original)

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R. bromii Related Products

Creative Biolabs also provide R. bromii products for researchers:

Product Name	Catalog No.	Target	Product Overview	Size	Price
Ruminococcus bromii Moore et al.	LBSX-0522-GF79	Ruminococcus	Ruminococcus bromii was isolated from human faeces.	-

FAQs

R. bromii acts as a keystone degrader of resistant starch in the human gut, initiating carbohydrate breakdown and supporting butyrate-producing partners through efficient cross-feeding mechanisms and short-chain fatty acid generation.

We process fecal or anaerobic enrichment samples under oxygen-free conditions, using resistant-starch–based media to selectively recover and characterize R. bromii strains with high starch-degrading and community-supporting capacity.

Yes. We deploy tiered co-cultures where R. bromii is paired with typical butyrogens and controls, monitoring pH, lactate, and SCFA to prevent confounding bloom dynamics while preserving interpretability.

We quantify adhesion to granular RS, hydrolysis rates, and soluble sugar release, then link these to cross-feeding responses in partner organisms and SCFA trends, yielding mechanism-anchored decision metrics.

Other Resources

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References

Ze, Xiaolei, et al. "Ruminococcus bromii is a keystone species for the degradation of resistant starch in the human colon." The ISME journal 6.8 (2012): 1535-1543. https://doi.org/10.1038/ismej.2012.4
Rangarajan, Aathmaja Anandhi, et al. "Ruminococcus bromii enables the growth of proximal Bacteroides thetaiotaomicron by releasing glucose during starch degradation." Microbiology 168.4 (2022): 001180. https://doi.org/10.1099/mic.0.001180
Cerqueira, Filipe M., et al. "Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome." Journal of Biological Chemistry 298.5 (2022). https://doi.org/10.1016/j.jbc.2022.101896

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Ruminococcus bromii Microbiome CRO Services