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Advanced Immune Disorder Animal Model Service for Probiotic Efficacy Evaluation

At Creative Biolabs, we specialize in providing comprehensive and scientifically rigorous preclinical services for the evaluation of probiotic efficacy in complex immune disorder animal models. With the burgeoning understanding of the gut-immune axis and the increasing interest in probiotics as therapeutic or prophylactic agents, our advanced services are designed to offer precise, reliable, and translatable data to accelerate your research and development. Whether you are exploring novel probiotic strains, optimizing formulations, or seeking to understand the underlying mechanisms of action, our expert team and state-of-the-art facilities are your ideal partners. Request a quote

Fig.1 Metabolic Disorder animal models for probiotic efficacy evaluation. (Creative Biolabs Authorized)

Overview: Bridging the Gap Between Gut Microbiota and Immune Health

Demonstrating the probiotics’ efficacy and understanding their precise mechanisms of action in a living system is crucial for their successful development and commercialization. Our service leverages a diverse portfolio of well-characterized animal models that faithfully mimic human immune disorders, allowing for the in-depth evaluation of your probiotic candidates. We go beyond basic readouts, providing a holistic assessment of immune parameters, gut barrier integrity, and microbiota composition to give you a complete picture of your probiotic's impact.

Comprehensive Immune Disorder Animal Model Services for Probiotic Efficacy Evaluation

Workflow

Fig.2 The workflow for the probiotic efficacy evaluation in Metabolic Disorder animal models. (Creative Biolabs original)

Service Details

Animal Models
Administration
Analysis
Samples
Deliverables
Turnaround Time

Animal Models

  • Inflammatory Bowel Disease (IBD) Models
    • DSS-induced Colitis (Acute & Chronic): A widely used model inducing colonic inflammation by disrupting the epithelial barrier.
    • TNBS-induced Colitis: Induces Th1-mediated inflammation, mimicking aspects of Crohn's disease.
    • IL-10 knockout (KO) mice: Develop spontaneous colitis due to a deficiency in the anti-inflammatory cytokine IL-10.
  • Autoimmune Disease Models
    • Experimental Autoimmune Encephalomyelitis (EAE): A widely used model for multiple sclerosis (MS), allowing investigation of central nervous system inflammation and demyelination.
    • Collagen-induced Arthritis (CIA): A model for rheumatoid arthritis (RA), assessing joint inflammation and damage.
    • Pristane-induced Lupus / MRL/lpr mice: Models for Systemic Lupus Erythematosus (SLE), studying systemic autoimmunity.
    • Type 1 Diabetes (e.g., NOD mice): Models for autoimmune diabetes.
  • Allergy & Hypersensitivity Models
    • OVA-induced Asthma/Allergic Airway Inflammation: Modeling respiratory allergies.
    • DNCB/OXA-induced Contact Hypersensitivity/Atopic Dermatitis: Modeling skin inflammatory conditions.

Probiotic Administration

  • Dosing Regimen: Determining optimal probiotic dose, frequency, and route of administration (oral gavage, dietary inclusion).
  • Control Groups: Appropriate vehicle, disease control, and positive control (known therapeutic) groups.
  • Timeline and Endpoints: Defining study duration and key parameters for evaluation.
  • Daily monitoring of animal health, body weight, and disease activity index (DAI), where applicable.

Comprehensive Endpoint Analysis

  • Sample Collection & Processing
    • Feces: For microbiota analysis (16S rRNA gene sequencing, metagenomics), SCFA quantification, and probiotic persistence.
    • Intestinal Tissues (Colon, Small Intestine): For histopathology, gene expression (qPCR, RNA-Seq), protein analysis (Western Blot, ELISA), immune cell phenotyping (flow cytometry), and gut barrier integrity markers (e.g., tight junction proteins).
    • Spleen, Lymph Nodes, Mesenteric Lymph Nodes: For immune cell isolation, phenotyping, and cytokine production assays.
    • Blood/Serum/Plasma: For systemic cytokine analysis, immune cell markers, and other relevant biomarkers.
    • Organ Tissues: Depending on the immune disorder (e.g., brain in EAE, joints in CIA), for histopathology and biomarker analysis.
  • Microbiota Analysis
    • 16S rRNA Gene Sequencing: To characterize bacterial community composition and diversity (alpha and beta diversity), identifying changes at the genus/species level.
    • Shotgun Metagenomics: For deeper functional insights, including gene pathways, microbial metabolic potential, and strain-level resolution.
    • Targeted qPCR: For quantification of specific probiotic strains or key microbial groups.
  • Immunological Assays
    • Cytokine & Chemokine Quantification (ELISA): Measuring pro-inflammatory and anti-inflammatory mediators in serum, tissue homogenates, and cell culture supernatants.
    • Flow Cytometry: Phenotyping and quantification of immune cell populations (T cells, B cells, macrophages, dendritic cells) in lymphoid organs and intestinal lamina propria.
    • Histopathology & Immunohistochemistry: Microscopic assessment of tissue damage, inflammation, immune cell infiltration, and specific protein expression in affected organs.
    • Gene Expression Analysis (qPCR, RNA-Seq): Quantifying expression of genes related to inflammation, immune response, and barrier function in tissues.
    • Myeloperoxidase (MPO) Activity: A marker for neutrophil infiltration and inflammation in intestinal tissues.
  • Gut Barrier Function Analysis
    • Transepithelial Electrical Resistance (TEER) in ex vivo Ussing Chamber studies: Direct measurement of intestinal barrier permeability.
    • FITC-Dextran Permeability Assay: In vivo assessment of gut leakiness.
    • Quantification of tight junction proteins (e.g., ZO-1, Occludin, Claudin) by Western Blot or IHC.
  • Metabolomics (Targeted/Untargeted)
    Analysis of SCFA and other microbial metabolites in fecal and serum samples.

Sample Information & Submission

  • Probiotic Strain(s) Information: Species, strain designation, viability (CFU/g or CFU/mL), storage conditions, and any relevant safety data (e.g., GRAS status).
  • Formulation Details: If the probiotic is part of a specific formulation (e.g., encapsulated, mixed with prebiotics).
  • Desired Route and Frequency of Administration: Based on your preliminary data or desired outcomes.
  • Specific Research Questions: What are you hoping to achieve or prove with this study?
  • Target Immune Disorder: Which specific condition are you interested in modeling?
  • Any preliminary in vitro data or existing hypotheses.

Deliverables

Deliverable Components Content Specifications
Comprehensive Study Report A detailed document outlining the experimental design, materials, methods, results (including raw data and processed figures), statistical analysis, and discussion of findings.
Raw Data Files Including sequencing data (FASTQ files for 16S/metagenomics), flow cytometry plots, ELISA raw values, and histological images.
Statistical Analysis Summary: Key statistical comparisons and significance.

Turnaround Time

Turnaround times are highly dependent on the complexity of the study, the chosen animal model, and the number of analytical endpoints. However, a typical study from initiation to final report delivery can range from 10 to 20 weeks. We will provide a precise timeline in your customized study proposal.

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Advantages of Partnering with Creative Biolabs

Expertise in Gut-Immune Axis

Deep scientific understanding of microbiota-host interactions and immune regulation.

Validated Animal Models

Access to a broad range of well-established and characterized immune disorder models.

Comprehensive Analytical Capabilities

State-of-the-art platforms for microbiology, immunology, histopathology, and metabolomics.

Customized Study Design

Flexible and tailor-made experimental plans can meet your specific research needs and budget requirements.

High-Quality Data & Interpretation

Rigorous scientific approach, robust statistical analysis, and clear, insightful reporting.

Ethical & Compliant Research

Adherence to the highest standards of animal welfare and ethical guidelines.

Dedicated Project Management

A single point of contact ensures smooth communication and the successful progress of the project.

Translational Focus

We are committed to bridging the gap between preclinical research results and potential human applications.

Fig.3 Advanced technical platforms are used for probiotic efficacy evaluation. (Creative Biolabs Authorized)

Applications

  • Probiotic Product Development: Testing the efficacy of novel probiotic strains or multi-strain formulations for various immune-related health claims.
  • Functional Food & Nutraceutical Industry: Validating the immunomodulatory properties of ingredients or finished products.
  • Pharmaceutical & Biotechnology Companies: Exploring probiotics as adjunctive therapies or standalone treatments for autoimmune and inflammatory diseases.
  • Academic Research Institutions: Providing specialized expertise and resources for complex in vivo studies on the gut microbiota and immune system.
  • Understanding Disease Pathogenesis: Elucidating the role of the microbiome in immune disorder development and progression.

Mechanism of Action: How Probiotics Influence Immune Health

  • Modulation of Gut Microbiota Composition: Probiotics can directly compete with pathogens, produce antimicrobial substances, and promote the growth of beneficial commensal bacteria, thereby shifting the overall microbial balance towards a healthier state.
  • Enhancement of Gut Barrier Integrity: Probiotics can strengthen the intestinal epithelial barrier, reducing "leaky gut" and preventing the translocation of harmful antigens and toxins that can trigger immune responses. This is often achieved through increased tight junction protein expression and mucin production.
  • Direct Interaction with Immune Cells: Probiotics can interact with various immune cells in the gut-associated lymphoid tissue (GALT), including dendritic cells, macrophages, T cells (e.g., regulatory T cells, Th17 cells), and B cells. This interaction can lead to the production of anti-inflammatory cytokines (e.g., IL-10, TGF-β) or the suppression of pro-inflammatory cytokines (e.g., TNF-α, IL-6, IFN-γ).
  • Production of Bioactive Metabolites: Probiotics produce a range of metabolites, such as short-chain fatty acids (SCFAs) like butyrate, propionate, and acetate, which serve as vital energy sources for colonocytes and possess potent anti-inflammatory and immunomodulatory properties.
  • Modulation of Host Signaling Pathways: Probiotics can influence various host signaling pathways involved in immune regulation, such as NF-κB, MAPK, and inflammasome pathways, ultimately leading to altered gene expression and protein production in immune cells.

Have you fully explored the full potential of the probiotic product you have chosen? Contact us today to schedule a confidential consultation and receive a customized proposal for your research needs.

FAQs

Can you help with custom animal model development if my target disease isn't listed?

Yes, our experienced team can discuss your specific needs and explore the feasibility of developing or adapting existing models.

What are the typical sample requirements for probiotic studies?

We typically require your probiotic material (powder, liquid, encapsulated), along with its viability (CFU/g or CFU/mL) and recommended storage conditions. We'll guide you on the exact quantities needed based on the study design.

What kind of data will I receive?

You will receive a comprehensive report including raw data, statistically analyzed results, figures, and an in-depth interpretation of the findings. We also provide raw data files for all assays (e.g., sequencing files, flow cytometry plots).

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