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Advanced Cancer Animal Model Service for Probiotic Efficacy Evaluation

At Creative Biolabs, we specialize in providing cutting-edge preclinical research services, with a particular focus on the intricate relationship between the microbiome, probiotics, and cancer. Our "Advanced Cancer Animal Model Service for Probiotic Efficacy Evaluation" is meticulously designed to accelerate your research into the anti-cancer potential of probiotic interventions. We offer comprehensive, high-quality studies that provide robust data to support your therapeutic development, attract investment, and advance regulatory submissions. Request a quote

Fig.1 Metabolic Disorder animal models for probiotic efficacy evaluation. (Creative Biolabs Authorized)

Overview: Unlocking the Therapeutic Potential of Probiotics in Cancer

The burgeoning field of microbiome research has illuminated the profound impact of gut microbiota on host health, including its significant role in cancer development, progression, and response to therapy. Probiotics are emerging as promising candidates for novel cancer interventions. They can modulate the immune system, alter metabolic pathways, and directly inhibit tumor growth. Our service provides a robust platform for evaluating the efficacy of your probiotic candidates in well-characterized in vivo cancer models. We go beyond basic efficacy, delving into the underlying mechanisms of action, ensuring you gain a deep understanding of your product's potential.

Comprehensive Cancer Animal Model Services for Probiotic Efficacy Evaluation

Workflow

Fig.2 The workflow for the probiotic efficacy evaluation in Metabolic Disorder animal models. (Creative Biolabs original)

Service Details

Animal Models
Administration
Analysis
Samples
Deliverables
Turnaround Time

Animal Models

  • Syngeneic Mouse Models: CT26 (colon), B16F10 (melanoma), 4T1 (breast), Lewis Lung Carcinoma (LLC), MC38 (colon), etc. These models are ideal for studying immune-mediated anti-tumor effects.
  • Xenograft Models: Human cancer cell lines (e.g., HCT116, HT29, A549, PC-3) implanted into immunodeficient mice (e.g., nude mice, SCID mice). Suitable for evaluating direct anti-proliferative effects.
  • Genetically Engineered Mouse Models (GEMMs): For studying cancer initiation and progression in a more physiologically relevant context.
  • Chemically-Induced Cancer Models: AOM/DSS-induced colorectal cancer, DMBA-induced breast cancer, etc.
  • Metastasis Models: Tail vein injection, splenic injection, orthotopic models to assess anti-metastatic potential.

Probiotic Administration

  • Oral gavage, dietary supplementation, enema, or other routes as per your study design.
  • Dose-ranging studies to determine optimal therapeutic concentrations.
  • Frequency and duration of administration are tailored to the model and research question.

Comprehensive Endpoint Analysis

  • Tumor Growth Monitoring
    • Regular caliper measurements for subcutaneous tumors.
    • Bioluminescence imaging (BLI) for orthotopic or metastatic models using luciferase-expressing cell lines.
    • Histopathological analysis of tumor tissues.
  • Immune System Analysis
    • Flow Cytometry: Comprehensive profiling of immune cell populations (T cells, B cells, NK cells, macrophages, dendritic cells, MDSCs, Tregs) in tumors, spleen, lymph nodes, and peripheral blood.
    • Cytokine/Chemokine Analysis: ELISA, or multiplex assays to quantify inflammatory and anti-inflammatory mediators in serum, plasma, and tissue homogenates.
    • Immunohistochemistry (IHC) & Immunofluorescence (IF): Spatial analysis of immune cell infiltration and expression of immune markers within tumor tissues.
    • ELISpot: Assessment of antigen-specific T cell responses.
  • Microbiome Analysis
  • Metabolomics & Proteomics
    • Analysis of host metabolites and proteins in various biological samples to identify pathways modulated by probiotic intervention.
  • Gene Expression Analysis
    • RT-qPCR or RNA-seq to evaluate the expression of key genes involved in cancer pathways, immune response, and metabolism in tumor and host tissues.
  • Histopathology & Pathology Scoring
    • H&E staining for general tissue morphology and tumor characteristics.
    • Specialized stains and pathological scoring for inflammation, necrosis, and fibrosis.
  • Toxicity and Safety Assessment
    • Body weight monitoring, clinical observations, organ weight, and gross pathology.
    • Blood biochemistry and hematology to assess systemic toxicity.

Sample Information & Submission (Client-Provided)

  • Probiotic Strain Information: Full taxonomic identification (genus, species, strain).
  • Formulation: Live culture, lyophilized powder, encapsulated, etc.
  • Viability/CFU Count: Current viable cell count per dose.
  • Storage Conditions: Recommended temperature, light, and humidity conditions.
  • Safety Data: Any existing in vitro or in vivo safety data, including GRAS status if applicable.
  • Mechanism of Action (Hypothesized): Any preliminary data or hypotheses regarding how your probiotic exerts its effects.
  • Target Indication: Specific cancer type(s) of interest.
  • Previous Studies: Any relevant preclinical or clinical data

Deliverables

Deliverable Components Content Specifications
Comprehensive Study Report
  • Executive Summary
  • Materials & Methods (detailed protocols)
  • Results (graphs, tables, statistical analysis)
  • Conclusion
  • Raw Data Appendices
Raw Data Files All raw data from various assays (e.g., flow cytometry FCS files, sequencing FASTQ files, ELISA raw values), Publication-quality graphical representations of key findings.

Turnaround Time

The turnaround time for each study is highly dependent on the complexity of the experimental design, the chosen cancer model, and the number of analyses requested.

Typical Total Project Duration: 12-25 weeks, depending on the specific project scope. We will provide a detailed timeline with your customized proposal.

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Advantages of Partnering with Creative Biolabs

Expertise

Our team comprises highly experienced scientists with deep knowledge in oncology, immunology, microbiology, and in vivo pharmacology.

State-of-the-Art Facilities

Access to cutting-edge animal facilities, advanced analytical platforms (flow cytometry, sequencing, mass spectrometry), and robust bioinformatics capabilities.

Customized Solutions

We understand that every probiotic and research question is unique. We offer highly flexible and customizable study designs tailored to your specific needs and budget.

Comprehensive Analysis

Beyond basic efficacy, we provide in-depth mechanistic insights into how your probiotic interacts with the host and tumor microenvironment.

Quality & Reproducibility

Strict adherence to guidelines, robust quality control measures, and rigorous statistical analysis ensure reliable and reproducible results.

Regulatory Support

Our data is generated to meet the high standards required for preclinical data packages, supporting your regulatory submissions.

Confidentiality

We maintain the highest standards of confidentiality and intellectual property protection for your proprietary research.

Fig.3 Advanced technical platforms are used for probiotic efficacy evaluation. (Creative Biolabs Authorized)

Applications

  • Preclinical Efficacy Screening: Identifying lead probiotic candidates with anti-cancer activity.
  • Mechanism of Action Studies: Elucidating how probiotics exert their therapeutic effects.
  • Combination Therapy Evaluation: Assessing the synergistic potential of probiotics with chemotherapy, radiotherapy, immunotherapy, or targeted therapies.
  • Biomarker Discovery: Identifying host or microbial biomarkers that predict response to probiotic intervention.
  • Safety and Tolerability Assessment: Evaluating the safety profile of probiotic candidates in in vivo models.
  • Formulation Optimization: Testing different probiotic formulations for optimal delivery and efficacy.
  • Supporting IND/CTA Filings: Generating robust preclinical data for regulatory submissions.

Mechanism of Action: How Probiotics Influence Cancer

Immune Modulation

  • Enhancing Anti-tumor Immunity: Probiotics can activate immune cells (e.g., T cells, NK cells, macrophages), promote the production of anti-tumor cytokines (e.g., IFN-γ, IL-12), and shift the immune microenvironment towards an anti-cancer phenotype.
  • Reducing Immunosuppression: They can mitigate the activity of immunosuppressive cells (e.g., Tregs, MDSCs) and reduce pro-tumorigenic cytokines (e.g., IL-10, TGF-β).

Metabolic Modulation

  • Short-Chain Fatty Acid (SCFA) Production: Probiotics, particularly those producing butyrate, acetate, and propionate, can directly inhibit cancer cell proliferation, induce apoptosis, and modulate epigenetic pathways.
  • Bile Acid Metabolism: Altering bile acid profiles can impact tumor growth and metastasis.
  • Nutrient Competition: Probiotics can compete with cancer cells for essential nutrients.

Direct Anti-tumor Effects

  • Apoptosis Induction: Certain probiotics can directly induce programmed cell death in cancer cells.
  • Cell Cycle Arrest: They can halt the cell cycle of tumor cells, preventing uncontrolled proliferation.
  • Anti-angiogenesis: Some probiotics may inhibit the formation of new blood vessels that feed tumors.

Gut Barrier Integrity

  • Reducing Endotoxemia: By strengthening the gut barrier, probiotics can reduce the translocation of bacterial toxins (e.g., LPS) that promote chronic inflammation, a known driver of cancer.

Modulation of Chemotherapy/Radiotherapy Efficacy and Toxicity

  • Probiotics can enhance the efficacy of conventional cancer treatments while mitigating their adverse side effects, particularly gut-related toxicities.

Ready to advance your probiotic cancer research? Contact us today for a confidential discussion about your project. Our scientific team is eager to collaborate with you to design a study that meets your specific objectives and accelerates your path to discovery.

FAQs

Can you work with my specific probiotic strain?

Yes, we are equipped to handle a diverse array of probiotic strains and formulations. We require detailed information on your product to ensure proper handling and study design.

Can you help with the interpretation of complex microbiome data?

Absolutely. Our bioinformatics team specializes in analyzing and interpreting complex 16S rRNA and shotgun metagenomic sequencing data, providing actionable insights into microbial community changes.

What is the typical cost of a study?

The cost varies significantly based on the complexity of the study design, the number of animals, the duration, and the analytical endpoints. We provide customized proposals after an initial consultation to ensure transparency.

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