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Metabolite Analysis based Probiotic Mechanism of Action (MOA) Study Service in Animal Models

At Creative Biolabs, we offer cutting-edge Metabolite Analysis-Based Probiotic Mechanism of Action (MOA) Study Services in Animal Models. Our comprehensive research solutions are designed to provide an in-depth understanding of how probiotic interventions exert their beneficial effects at a molecular level, empowering your R&D efforts and accelerating product development. By integrating advanced metabolomics with robust animal model studies, we provide unparalleled insights into the intricate interplay between probiotics, host metabolism, and disease states. Request a quote

Fig.1 Animal models for probiotic mechanism of action (MOA) study. (Creative Biolabs Authorized)

Overview

Metabolomics, the large-scale study of small molecules (metabolites) within a biological system, offers a powerful lens to decode these complex interactions. Our service leverages state-of-the-art metabolomic platforms in conjunction with well-controlled animal models to identify, quantify, and characterize the metabolic changes induced by probiotics, shedding light on their MOA. This service is crucial for:

  • Validating health claims: Providing scientific evidence to support the efficacy of your probiotic products.
  • Optimizing probiotic strains: Identifying specific strains or combinations with superior metabolic impact.
  • Understanding host-microbe interactions: Deciphering the complex communication pathways between probiotics and the host.
  • Discovering novel biomarkers: Uncovering metabolite signatures associated with probiotic-induced health benefits.
  • Accelerating product development: Reducing time and cost by pinpointing effective mechanisms early in the research pipeline.

Animal Model Probiotic MOA: Metabolite Analysis Services for Deeper Insights

Workflow

Fig.2 The workflow of the Metabolite Analysis for the probiotic MOA study in animal models. (Creative Biolabs original)

Service Details

Animal Models
Administration
Analysis
Samples
Deliverables
Turnaround Time

Animal Models

  • Species: Rodents (mice, rats), often preferred for their genetic tractability and established disease models. Other species are available upon request.
  • Disease Models:
    • Gastrointestinal disorders (e.g., IBD, IBS, antibiotic-associated diarrhea)
    • Metabolic diseases (e.g., obesity, diabetes, non-alcoholic fatty liver disease)
    • Immune-related conditions (e.g., allergies, autoimmune diseases)
    • Neurological disorders (e.g., anxiety, depression, cognitive function via gut-brain axis)
    • Infectious diseases
  • Sample Collection and Preparation
    • Biological Fluids: Plasma, serum, urine, bile, cerebrospinal fluid.
    • Tissues: Intestinal mucosa, liver, muscle, adipose tissue, brain, spleen, and kidney.
    • Fecal Samples: For gut microbiota composition analysis and microbial metabolite profiling.
    • Optimal preservation techniques to maintain metabolite integrity.

Probiotic Administration & Dosing

Oral gavage, dietary inclusion, or other suitable methods.

Metabolomic Profiling

  • Untargeted Metabolomics: Comprehensive discovery of novel metabolites and pathways using advanced mass spectrometry (LC-MS/MS, GC-MS) and NMR spectroscopy. Ideal for hypothesis generation.
  • Targeted Metabolomics: Quantitative analysis of specific classes of metabolites (e.g., SCFAs, bile acids, amino acids, lipids, neurotransmitters, organic acids). Ideal for validating specific hypotheses and identifying key biomarkers.
  • Microbiota Analysis (Optional but Recommended)

16S rRNA gene sequencing or metagenomics to characterize changes in gut microbial composition and diversity. This provides crucial context for interpreting metabolomic data, linking microbial shifts to metabolic alterations.

Sample Information

  • Sample Type: We accept a wide range of biological samples, including plasma, serum, urine, feces, liver tissue, intestinal tissue, brain tissue, and more.
  • Sample Quantity: Specific requirements vary by sample type and analytical platform. Detailed guidelines will be provided during the study design phase.
  • Sample Handling & Shipping: Strict protocols for freezing, labeling, and shipping samples to ensure their integrity upon arrival at our facility. Dry ice shipping is typically required.

Deliverables

Upon completion of your study, you will receive a comprehensive package including:

Deliverable Components Content Specifications
Detailed Study Report
  • Executive summary
  • Detailed methodology (animal care, dosing, sample collection, analytical methods)
  • Raw data processing and quality control reports
  • Statistical analysis results (univariate and multivariate)
  • Identified and quantified metabolite lists with fold changes and statistical significance
  • Metabolic pathway analysis and enrichment maps
  • Biological interpretation of results, highlighting key insights into probiotic MOA
  • Conclusions and recommendations for future research.
Raw Data Files All original data was generated from the analytical platforms.
Graphical Representations High-quality figures and plots illustrating significant findings.

Turnaround Time

The turnaround time for Metabolite Analysis-Based Probiotic MOA Studies varies depending on the complexity of the study design, number of samples, and the depth of analysis required. Specific timelines will be provided in your custom proposal. We prioritize timely communication and provide regular updates on project progress.

Contact Us Today for a Quote

Advantages of Partnering with Creative Biolabs

Expertise in Probiotic Research

Our team comprises experienced scientists with deep knowledge in microbiology, animal physiology, and metabolomics, specifically in the context of probiotic research.

State-of-the-Art Platforms

Access to the latest high-resolution mass spectrometry and NMR instruments, ensuring accurate and comprehensive metabolite profiling.

Robust Animal Models

Established protocols for various relevant animal disease models, offering high reproducibility and clinical translatability.

Integrated Omics Approach

Ability to combine metabolomics with other omics technologies (e.g., metagenomics) for a holistic understanding.

Customizable Solutions

Flexible service offerings tailored to your unique research questions and budget.

Comprehensive Data Analysis

Advanced bioinformatics and statistical capabilities to extract meaningful biological insights from complex data.

High-Quality Deliverables

Professional, publication-ready reports and well-organized data for seamless integration into your research.

Dedicated Project Management

Establish a single contact point to ensure smooth communication and the efficient execution of the project.

Strict Quality Control

Adherence to rigorous quality control standards throughout the entire workflow to ensure data reliability and reproducibility.

Fig.3 Advanced omics platforms for probiotic MOA study. (Creative Biolabs Authorized)

Applications

  • Pharmaceutical Companies: Investigating new probiotic strains as therapeutic agents for various diseases.
  • Nutraceutical and Functional Food Companies: Substantiating health claims for probiotic-enriched products.
  • Animal Health Companies: Developing probiotics for livestock and companion animals to improve health and performance.
  • Academic Institutions: Conducting fundamental research into host-microbe interactions and metabolic regulation.
  • Biotechnology Startups: Early-stage validation and characterization of novel probiotic candidates.

Understanding Probiotic Mechanism of Action (MOA) through Metabolomics

Probiotics exert their effects through various mechanisms, many of which involve the production or modulation of metabolites. These can include:

  • Short-chain fatty acids (SCFAs): Such as butyrate, propionate, and acetate, which play crucial roles in gut health, immune modulation, and energy metabolism.
  • Bile acid modification: Altering bile acid profiles, impacting lipid metabolism and gut signaling.
  • Neurotransmitters and neuromodulators: Influencing the gut-brain axis.
  • Vitamins and amino acids: Synthesizing essential nutrients for the host.
  • Antimicrobial compounds: Producing bacteriocins and other substances that inhibit pathogen growth.
  • Modulation of host metabolic pathways: Directly or indirectly affecting host energy metabolism, inflammation, and immune responses.

Ready to unlock the full potential of your probiotic interventions? Contact us today for a free consultation and a tailor-made solutions.

FAQs

What types of probiotics can you study?

We can study a wide range of probiotic strains, including bacteria (e.g., Lactobacillus, Bifidobacterium, Streptococcus), yeasts, and multi-strain formulations.

Is microbiota analysis included in the service?

Microbiota analysis (e.g., 16S rRNA sequencing or metagenomics) is an optional but highly recommended add-on service. Combining the data of the microbial community with the metabolomics data can provide a more comprehensive understanding of the effects of probiotics.

How do you ensure the quality and reproducibility of the data?

We implement stringent quality control measures at every step, from sample collection and preparation to instrumental analysis and data processing. This includes using internal standards, replicate injections, and robust statistical methods to ensure data reliability.

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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.

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