How is Bifidobacterium bifidum powder typically rehydrated for experimental use?

Bifidobacterium bifidum powder is rehydrated by suspending it in an anaerobic growth medium at a suitable concentration. The rehydration process typically involves gently mixing the powder in the medium and incubating it under anaerobic conditions to revive the bacteria.

What is the recommended procedure for preparing Bifidobacterium bifidum powder for in vivo studies?

For in vivo studies, Bifidobacterium bifidum powder should be rehydrated in a sterile physiological solution or appropriate vehicle. The suspension can then be administered to test subjects via oral gavage or included in their diet, ensuring consistent dosing throughout the study.

What are the common challenges faced when working with Bifidobacterium bifidum powder, and how can they be addressed?

Common challenges include maintaining anaerobic conditions during rehydration and preventing contamination. These can be addressed by using an anaerobic chamber or jars and following strict aseptic techniques. Additionally, ensuring proper storage at -20°C or lower helps maintain viability.

How should the rehydrated Bifidobacterium bifidum be quantified for experimental applications?

Quantification of rehydrated Bifidobacterium bifidum can be performed using plate counting methods on selective media or flow cytometry. This allows accurate determination of colony-forming units (CFU) per milliliter, ensuring precise dosing in experiments.

What types of media are recommended for culturing Bifidobacterium bifidum from its powder form?

Recommended media for culturing Bifidobacterium bifidum include MRS broth supplemented with cysteine or other anaerobic media tailored for bifidobacterial growth. These media provide essential nutrients and maintain the anaerobic environment required for optimal growth.

Datasheet

Bifidobacterium bifidum Powder (CAT#: LBP-008CYG)

Freeze-dried Bifidobacterium bifidum Powder

This product is a raw material for food manufacturing. It is not intended for direct consumption and is not sold to retail or individual customers.

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Product Information
Product Overview	Freeze-dried Bifidobacterium bifidum Powder
Product Type	Raw material
Grade	Food grade
Target	Bifidobacterium
Source	Human
Genetic Modifications	None
Appearance	White to light yellow powder
Size	1kg; 5Kg; 10Kg; 25Kg; other.
MOQ	1Kg
Storage	Store at -20°C to -80°C.
Shelf Life	24 months
Note	1. Characters of the strain are genetically identified. 2. Types of probiotic blends/premixes could be provided based on the clients' requirements. 3. Tyndalized (Heat-Sterilized) specifications could be provided based on clients' requirements. 4. Complete data sheet is available based on the clients' requirements.

Target Introduction
Introduction	Bifidobacterium bifidum is a bacterial species of the genus Bifidobacterium. B. bifidum is one of the most common probiotic bacteria that can be found in the body of mammals, including humans. B. bifidum is a Gram-positive bacterium that is not motile, anaerobic, and not spore-forming. The bacterium is rod-shaped and can be found living in clusters, pairs, or even independently. B. bifidum is an essential bacteria found in the human intestine. When it is low or absent altogether in the human intestine, it is an indication of being in an unhealthy state. Intestinal flora can be improved if someone takes oral B. bifidum.
Alternative Names	Bifidobacterium bifidum; B. bifidum

Target Introduction

Introduction

Bifidobacterium bifidum is a bacterial species of the genus Bifidobacterium. B. bifidum is one of the most common probiotic bacteria that can be found in the body of mammals, including humans. B. bifidum is a Gram-positive bacterium that is not motile, anaerobic, and not spore-forming. The bacterium is rod-shaped and can be found living in clusters, pairs, or even independently. B. bifidum is an essential bacteria found in the human intestine. When it is low or absent altogether in the human intestine, it is an indication of being in an unhealthy state. Intestinal flora can be improved if someone takes oral B. bifidum.

Alternative Names

Bifidobacterium bifidum; B. bifidum

1Food and Beverage Industry

Dairy Products: Yogurt, kefir, probiotic drinks, cheese, ice cream, and dairy desserts.
Non-Dairy Alternatives: Plant-based yogurts (soy, almond, coconut, oat), probiotic beverages (juices, smoothies, fermented plant-based drinks).
Baked Goods: Bread, snacks, and other baked items.
Confectionery: Probiotic chocolates, gummies, and other sweets.
Functional Foods and Beverages: Products specifically designed to offer health benefits beyond basic nutrition.

2Dietary Supplements

Capsules and Tablets: Single-strain or multi-strain formulations with varying dosages.
Powders: For mixing with water, smoothies, or other foods.
Liquids and Drops.

3Animal Feed

Although not for human consumption, food-grade probiotics are also used in animal feed.

For Preclinical Animal Research Use (Gavage study), Please inquire about our customized lab-scale probiotic production services.

How is Bifidobacterium bifidum powder typically rehydrated for experimental use?
Bifidobacterium bifidum powder is rehydrated by suspending it in an anaerobic growth medium at a suitable concentration. The rehydration process typically involves gently mixing the powder in the medium and incubating it under anaerobic conditions to revive the bacteria.
What is the recommended procedure for preparing Bifidobacterium bifidum powder for in vivo studies?
For in vivo studies, Bifidobacterium bifidum powder should be rehydrated in a sterile physiological solution or appropriate vehicle. The suspension can then be administered to test subjects via oral gavage or included in their diet, ensuring consistent dosing throughout the study.
What are the common challenges faced when working with Bifidobacterium bifidum powder, and how can they be addressed?
Common challenges include maintaining anaerobic conditions during rehydration and preventing contamination. These can be addressed by using an anaerobic chamber or jars and following strict aseptic techniques. Additionally, ensuring proper storage at -20°C or lower helps maintain viability.
How should the rehydrated Bifidobacterium bifidum be quantified for experimental applications?
Quantification of rehydrated Bifidobacterium bifidum can be performed using plate counting methods on selective media or flow cytometry. This allows accurate determination of colony-forming units (CFU) per milliliter, ensuring precise dosing in experiments.
What types of media are recommended for culturing Bifidobacterium bifidum from its powder form?
Recommended media for culturing Bifidobacterium bifidum include MRS broth supplemented with cysteine or other anaerobic media tailored for bifidobacterial growth. These media provide essential nutrients and maintain the anaerobic environment required for optimal growth.

Exceptional Quality for Gut Health Research
Creative Biolabs' Bifidobacterium bifidum powder offers exceptional quality, greatly enhancing the reliability of gut health studies.
Superior Viability and Stability
The Bifidobacterium bifidum powder from Creative Biolabs ensures superior viability and stability, crucial for consistent results in our gastrointestinal research.
Outstanding Consistency
Creative Biolabs' Bifidobacterium bifidum powder consistently delivers outstanding results, making it a preferred choice for microbiome research.
Effortless Integration in Research Protocols
Creative Biolabs' Bifidobacterium bifidum powder integrates effortlessly into our research protocols, simplifying our workflow and improving efficiency.
User-Friendly and Effective
The user-friendly nature of Creative Biolabs' Bifidobacterium bifidum powder ensures smooth application in our lab processes, enhancing overall research productivity.

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This product is a raw material for food manufacturing. It is not intended for direct consumption and is not sold to retail or individual customers.