Freeze-dried Bifidobacterium bifidum Powder
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information | |
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Product Overview | Freeze-dried Bifidobacterium bifidum Powder |
Target | Bifidobacterium |
Source | Human |
CFU Value | 2×10^11 |
Genetic Modifications | None |
Application | Used as live bacteria probiotic |
Appearance | White to light yellow powder |
Size | 1 kg |
Storage | Store at -20°C to -80°C. |
Shelf Life | 24 months |
Note | 1. Characters of the strain are genetically identified. 2. Types of probiotic blends/premixes could be provided based on the clients' requirements. 3. Tyndalized (Heat-Sterilized) specifications could be provided based on clients' requirements. 4. Complete data sheet is available based on the clients' requirements. 5. For research use only. Not intended for food, diagnostics, therapeutic or any in vivo human use. |
Target Introduction | |
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Introduction | Bifidobacterium bifidum is a bacterial species of the genus Bifidobacterium. B. bifidum is one of the most common probiotic bacteria that can be found in the body of mammals, including humans. B. bifidum is a Gram-positive bacterium that is not motile, anaerobic, and not spore-forming. The bacterium is rod-shaped and can be found living in clusters, pairs, or even independently. B. bifidum is an essential bacteria found in the human intestine. When it is low or absent altogether in the human intestine, it is an indication of being in an unhealthy state. Intestinal flora can be improved if someone takes oral B. bifidum. |
Alternative Names | Bifidobacterium bifidum; B. bifidum |
Bifidobacterium bifidum powder is rehydrated by suspending it in an anaerobic growth medium at a suitable concentration. The rehydration process typically involves gently mixing the powder in the medium and incubating it under anaerobic conditions to revive the bacteria.
For in vivo studies, Bifidobacterium bifidum powder should be rehydrated in a sterile physiological solution or appropriate vehicle. The suspension can then be administered to test subjects via oral gavage or included in their diet, ensuring consistent dosing throughout the study.
Common challenges include maintaining anaerobic conditions during rehydration and preventing contamination. These can be addressed by using an anaerobic chamber or jars and following strict aseptic techniques. Additionally, ensuring proper storage at -20°C or lower helps maintain viability.
Quantification of rehydrated Bifidobacterium bifidum can be performed using plate counting methods on selective media or flow cytometry. This allows accurate determination of colony-forming units (CFU) per milliliter, ensuring precise dosing in experiments.
Recommended media for culturing Bifidobacterium bifidum include MRS broth supplemented with cysteine or other anaerobic media tailored for bifidobacterial growth. These media provide essential nutrients and maintain the anaerobic environment required for optimal growth.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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