Alistipes finegoldii; 00980 (CAT#: LBGF-0722-GF42)

Alistipes finegoldii is an anaerobic, Gram-negative bacterium predominantly found in the human gut. It is of particular interest in microbiome research due to its potential role in various health conditions, including inflammatory diseases, colorectal cancer, and metabolic disorders. This strain is widely used in studies investigating gut microbiota composition and function.

This strain grows best under strict anaerobic conditions at 37°C, typically using media like Columbia blood agar. It is bile-resistant and requires specific growth media rich in vitamins, which makes its cultivation challenging in some experimental setups. Cultivation must ensure oxygen-free environments to maintain viability.

Yes, Alistipes finegoldii has been used in gut barrier function studies. Its role in producing metabolites that support gut integrity makes it a focus in models investigating leaky gut syndrome. Researchers study its effects on the epithelial cells lining the gut, looking at its potential to either maintain or disrupt gut barrier health.

To study immune modulation, researchers often use in vitro assays involving co-cultures with immune cells. This helps evaluate the bacterium's impact on cytokine production, specifically its influence on pro- and anti-inflammatory cytokines like IL-6 and TNF-α. These assays provide insights into how Alistipes finegoldii interacts with the host immune system.

Researchers commonly use quantitative techniques such as real-time PCR, flow cytometry, or colony-forming unit (CFU) counts to measure the abundance of Alistipes finegoldii. These methods allow for precise quantification of the strain in microbiome and gut health studies, enabling researchers to assess its impact under various experimental conditions.