Zebrafish Model related Service for Gut Microbiota Study
Background Services Advantages Published Data FAQs
Background
The gut microbiota plays a crucial role in maintaining host health. Many human diseases are related to imbalanced gut microbiota. Probiotics can help maintain a healthy balance of microbes in the gut, which may serve as an adjunct to preventing and mitigating adverse drug reactions. Therefore, it is very important to use experimental animal models to verify the physiological or pathological functions of intestinal microbiota changes. As a low-cost, genetically susceptible vertebrate model, zebrafish has been widely used in biological research. It shares some similarities with humans in gut physiology and function, which makes the zebrafish useful for studying the interactions between the gut microbiome and its host. The use of zebrafish in high-throughput research can provide a deeper understanding of the complex chain between the gut microbiome and the host system, leading to a better understanding of the pathophysiology of human diseases and the discovery of more effective therapeutic targets. Fully tapping into the potential of zebrafish in the gut microbiota will provide more methods for the treatment of diseases.
Services for the Versatility of Zebrafish for Diseases Studies
Due to the similarities in gut and nervous system function as well as microbiome composition between mammals and zebrafish, zebrafish is an attractive model for studying host-microbiome interactions in neurological health.
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The zebrafish microbiome serves as a functional biomarker for host immune response.
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Study the interactions of probiotics and hosts with different gut flora and infectious diseases.
Derivative services available for the research project:
Functional Studies of Intestinal Microbiota Using Zebrafish Models
Some studies describe the molecular mechanisms behind host-microbe interactions through biological models.
The development of zebrafish requires the colonization and composition of early intestinal flora.
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Reproduction
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Immunity
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Metabolism
Given the sharing of a large number of metabolic-related genes with humans, zebrafish models have been widely used to study the relationship between metabolic diseases and gut microbiota.
The microbiota plays a crucial role in maintaining host behavior by regulating brain function and altering basic behavioral patterns.
Fig.1 Physiological function comparison of gut microbiota between zebrafish and humans.1, 3
Advantages of Zebrafish Models
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Allows real-time in vivo imaging capabilities at the whole organism level.
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Powerful and simple GF-rearing, gnotobiotic conditions are available.
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Availability of transgenic lines expressing fluorescent proteins.
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It's easier to manipulate zebrafish with specific genes or microbiomes.
Published Data
Gut microbiota alteration by Lactobacillusrhamnosus reduces pro-inflammatory cytokines and glucose levels in the adult model of Zebrafish2, 3
Abstract
To investigate the effects of Lactobacillus rhamnosus as a probiotic on type 2 diabetes, scientists developed a diet-induced diabetic zebrafish model, and the results suggest that probiotics such as Lactobacillus rhamnosus may inhibit blood glucose increase in T2DM groups through immunomodulatory effects. Therefore, the development of a probiotic-based treatment formula could promote the health of people with type 2 diabetes.
Frequently Asked Questions
Can zebrafish models be used to study gut microbiota?
Absolutely. Zebrafish models are extensively used to study gut microbiota due to their many advantageous features:
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Transparency: Zebrafish larvae are transparent, allowing researchers to visually track gut microbiota development and interactions in real-time.
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Gnotobiotic Techniques: Researchers can raise zebrafish in germ-free environments and then introduce specific microbial communities to study their effects.
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Genetic and Physiological Similarity to Humans: Making them relevant models for human gut microbiota studies.
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Rapid Development: Zebrafish develop quickly, which means researchers can observe the impacts of gut microbiota over shorter time spans compared to other models.
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Disease Modeling: They are used to model various diseases related to gut microbiota to investigate how probiotics and other treatments might help.
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High Reproduction Rate: Their high fecundity allows for large-scale studies, providing robust data sets.
In these ways, zebrafish models are an invaluable resource in gut microbiota research, offering insights that can translate to human health and disease.
Can zebrafish models be given freeze-dried probiotics?
Yes, zebrafish models can be given freeze-dried probiotics. Freeze drying is a common method for preserving probiotics and can be effectively used in zebrafish research. Researchers often incorporate freeze-dried probiotics into the fish feed or water to study their effects on gut microbiota, immune response, and overall health.
Creative Biolabs has built zebrafish CRO platforms to develop pathological models and evaluate drug efficacy and safety. If you have any specific research involving zebrafish models, please do not hesitate to contact us for more details and we will respond to your request for a quotation or project proposal as soon as possible.
References
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Xia, Hui, et al. "Zebrafish: an efficient vertebrate model for understanding role of gut microbiota." Molecular Medicine 28.1 (2022): 161.
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Bootorabi, Fatemeh, et al. "Gut micobiota alteration by Lactobacillus rhamnosus reduces pro-inflammatory cytokines and glucose level in the adult model of Zebrafish." BMC Research Notes 14.1 (2021): 302.
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Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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