In the rapidly evolving landscape of nutritional science and pharmaceuticals, the demand for robust, reliable, and relevant preclinical models to evaluate the efficacy of probiotics in mitigating chronic inflammation has never been higher. Creative Biolabs specializes in providing state-of-the-art Advanced Chronic Inflammatory Animal Model Services specifically designed for comprehensive probiotic efficacy evaluation. We offer a meticulously crafted suite of research services aimed at accelerating your product development and providing definitive data to support your claims. Request a quote
Overview
Chronic inflammation is a significant contributor to a wide range of debilitating diseases, including inflammatory bowel disease (IBD), rheumatoid arthritis, metabolic syndrome, and neurodegenerative disorders. Probiotics are increasingly recognized for their potential to modulate immune responses and reduce inflammation. Our service utilizes carefully validated animal models that reliably mimic the complex pathophysiology of human chronic inflammatory conditions, allowing for precise and predictive assessment of your probiotic formulations. We offer a holistic approach, from study design to data analysis, ensuring high-quality, actionable results.
Comprehensive Chronic Inflammatory Animal Model Services for Probiotic Efficacy Evaluation
Workflow
Service Details
Animal Models
Administration
Analysis
Samples
Deliverables
Turnaround Time
Animal Models
-
Inflammatory Bowel Disease (IBD) Models
-
DSS-induced Colitis (Dextran Sulfate Sodium): Acute and chronic models mimicking ulcerative colitis, enabling evaluation of probiotic effects on mucosal inflammation, barrier function, and clinical parameters.
-
TNBS-induced Colitis (Trinitrobenzene Sulfonic Acid): Models reflecting Crohn's disease-like inflammation, ideal for assessing transmural inflammation and fibrosis.
-
MDR1a Knockout Mice: Spontaneous model of chronic colitis, useful for long-term probiotic intervention studies.
-
Rheumatoid Arthritis Models
-
Collagen-Induced Arthritis (CIA): Gold standard model for RA, allowing evaluation of joint inflammation, damage, and systemic immune responses.
-
Adjuvant-Induced Arthritis (AIA): Another robust model for assessing anti-inflammatory and immunomodulatory effects.
-
Metabolic Syndrome/Obesity-Induced Inflammation Models
-
High-Fat Diet (HFD) Induced Obesity and Inflammation: For evaluating probiotics' impact on adipose tissue inflammation, insulin resistance, and liver steatosis.
-
Neuroinflammation Models
-
LPS-induced Neuroinflammation: Acute and chronic models for assessing neuroprotective effects and modulation of neuroinflammatory markers.
-
Other Custom Models: Development and validation of bespoke inflammatory models based on client-specific needs.
Probiotic Administration
-
Oral gavage, dietary supplementation, or other appropriate routes of administration.
-
Careful management of probiotic viability and stability throughout the study.
-
Dose-response studies to determine optimal probiotic concentrations
Comprehensive Endpoint Analysis
-
Biomarker Analysis
-
Inflammatory Cytokines: ELISA for IL-6, TNF-α, IL-1β, IL-10, TGF-β, IFN-γ.
-
Chemokines: MCP-1, MIP-2, KC.
-
Acute Phase Proteins: CRP, SAA.
-
Oxidative Stress Markers: MDA, MPO, antioxidant enzyme activity (SOD, GSH).
-
Gut Barrier Integrity Markers: FITC-dextran permeability assay, zonulin, occludin, claudin expression.
-
Metabolic Markers: Glucose, insulin, lipid profiles, HOMA-IR.
-
Fecal Calprotectin: A non-invasive marker of intestinal inflammation.
-
Histopathological Evaluation
-
Gross pathology and macroscopic scoring of tissues.
-
Microscopic evaluation of stained tissue sections (H&E, PAS, Masson's Trichrome for fibrosis).
-
Quantitative scoring of inflammation, immune cell infiltration, tissue damage, and regeneration.
-
Immunohistochemistry (IHC) or Immunofluorescence (IF) for specific cell types (e.g., T cells, B cells, macrophages) and protein markers.
-
Microbiota Analysis
-
Short-Chain Fatty Acid (SCFA) Analysis
GC-MS analysis of fecal or cecal SCFAs (acetate, propionate, butyrate, etc.).
-
Gene Expression Analysis (qPCR)
Quantification of mRNA levels for key inflammatory mediators, tight junction proteins, and immune cell markers in relevant tissues.
Sample Information & Submission (Client-Provided)
-
Probiotic Strain(s): Detailed information including species, strain, concentration (CFU/g or CFU/mL), storage conditions, and stability data.
-
Formulation: Information on the final product formulation (e.g., powder, capsule, liquid) and any excipients.
-
Vehicle: Preferred vehicle for administration (e.g., saline, water, specific diet).
-
Safety Data: Any existing safety data on the probiotic, if available.
-
Specific Research Questions: Clear articulation of the key questions you aim to answer through the study.
Deliverables
|
Deliverable Components
|
Content Specifications
|
|
Comprehensive Study Report
|
-
Executive Summary.
-
Materials and Methods.
-
Detailed Results (with statistical analysis).
-
Conclusions and Interpretation.
|
|
Raw Data Files
|
Clinical scores, body weights, ELISA readings, sequencing data, processed and analyzed data tables and graphs, high-resolution histological images with quantitative scoring
|
Turnaround Time
Turnaround times vary depending on the complexity of the study, the chosen animal model, and the number of endpoints. A typical study involving a chronic inflammatory model and comprehensive analysis can range from 8 to 16 weeks from study initiation to final report delivery. Specific timelines will be provided in your customized project proposal.
Contact Us Today for a Quote
Advantages of Partnering with Creative Biolabs
Expertise and Experience
Our team comprises highly skilled scientists and veterinarians with extensive experience in chronic inflammatory disease models and probiotic research.
Validated Models
We utilize well-established and rigorously validated animal models that accurately reflect human disease pathology.
Comprehensive Capabilities
From study design to advanced molecular analysis, we offer an end-to-end solution.
Customization
Flexible study design tailored to your unique probiotic and research objectives.
State-of-the-Art Facilities
Access to accredited animal facilities and cutting-edge laboratory equipment.
Quality and Reproducibility
Strict adherence to principles ensures high data quality, reproducibility, and integrity.
Regulatory Support
Our data is generated to a standard suitable for supporting regulatory submissions.
Confidentiality
Strict adherence to confidentiality agreements and intellectual property protection.
Applications
-
Nutraceutical and Functional Food Companies: Demonstrating the health benefits of probiotic-containing products for chronic inflammatory conditions.
-
Pharmaceutical Companies: Identifying novel probiotic strains or combination therapies with anti-inflammatory potential.
-
Biotechnology Companies: Validating the efficacy of genetically engineered probiotics.
-
Academic Researchers: Generating robust preclinical data for grant applications and publications.
-
Veterinary Health Companies: Developing probiotics for inflammatory conditions in companion animals or livestock.
Mechanism of Action (How Probiotics Address Chronic Inflammation)
-
Modulation of Gut Microbiota Composition
Probiotics can restore microbial homeostasis, inhibit the growth of pathogenic bacteria, and promote beneficial commensals, thereby reducing dysbiosis-associated inflammation.
-
Strengthening Gut Barrier Function
Many probiotics enhance tight junction integrity, reducing gut permeability ("leaky gut") and preventing the translocation of bacterial products (e.g., LPS) that trigger systemic inflammation.
Probiotics can interact directly with immune cells in the gut-associated lymphoid tissue (GALT), influencing the differentiation and function of T regulatory cells (Tregs), Th17 cells, and cytokine production (e.g., IL-10, TGF-β, TNF-α, IL-6).
-
Production of Short-Chain Fatty Acids (SCFAs)
Probiotics, particularly those that ferment dietary fiber, produce SCFAs like butyrate, propionate, and acetate. SCFAs serve as energy sources for colonocytes, possess anti-inflammatory properties, and can modulate immune cell function.
Some probiotic strains exhibit antioxidant properties, directly neutralizing reactive oxygen species (ROS) and reducing oxidative stress, a key driver of chronic inflammation.
-
Competition for Adhesion Sites and Nutrients
Probiotics can compete with pathogens for colonization of the intestinal mucosa and essential nutrients, thereby reducing pathogen-induced inflammation.
Ready to advance your probiotic research? Contact us today to discuss your specific project needs and receive a customized proposal.
FAQs
What types of probiotics can you evaluate?
We can evaluate a wide range of probiotics, including single-strain products, multi-strain blends, and spore-forming probiotics. We can also accommodate novel live biotherapeutic products.
Can you help with strain selection?
While our primary service is efficacy evaluation, our expert team can provide guidance on strain characteristics and existing literature to inform your strain selection process.
Can I provide my specialized diet or vehicle for administration?
Yes, we are flexible and can accommodate client-provided diets or vehicles, provided they meet our safety and logistical requirements.
What level of detail can I expect in the final report?
Our final reports are comprehensive and include detailed methodologies, all raw and analyzed data, statistical interpretations, and clear conclusions. We strive to provide all the information you need for regulatory submissions or internal decision-making.
