Metatranscriptomics Profiling

Metatranscriptomics is to investigate valuable information about the whole gene expression pattern of complex microbial communities. It is widely applied for microbial community analysis. With expertise in next-generation sequencing, Creative Biolabs offers a full range of metatranscriptomic sequencing service package including sample preparation, library construction, sequencing, bioinformatics analysis to support accurate microbial identification.

Introduction of Metatranscriptomics Profiling

Metatranscriptomics is to study the entire gene expression profiling of complex microbial communities of an ecosystem. It is a subset of metagenomics and a complementary approach to metagenomics. Metagenomics mainly investigates the whole genomes of a microbial community and determine the contents of the genome. While metatranscriptomics focuses on the diversity and expression profile of active genes in such community and how these levels vary with environmental conditions. Compared with metagenomics, metatranscriptomics can study the changes of a complex microbial community from a transcriptome perspective, and can better explore the potential novel genes, differentially expressed genes, and their functions as well as the construction of metabolic pathways.

Our Capacities

Creative Biolabs has established a standardized operating laboratory and a high-throughput sequencing technology platform with Illumina HiSeq 2500, HiSeq 4000, and other high-throughput sequencing instruments. Our professional experts have accumulated rich experience in next-generation sequencing and can provide the best and comprehensive metatranscriptomic sequencing and analysis services to illustrate the function and activity of the complete set of transcripts, as well as community structure of a microbial community.

Workflow

The analysis includes but not limited:

• Raw data quality control: filtering out low-quality data
• De novo assembly and reads alignment based on the reference genome
• Species and gene function annotation as well as taxonomic analysis
• Unigene clusterings analysis including GO and KEGG annotations
• Unigene abundance and coverage analysis
• Differential expression analysis
• Gene variation analysis (such as coding SNPs, SSRs, and ORFs)

Sample Requirements

• Sample type: total RNA
• RNA concentration ≥200 ng/μl
• RNA amount ≥3 μg
• Purity: OD260/280=1.8~2.2, OD260/230 ≥1.0; without degradation, no DNA or protein contamination
• Repeated freezing-thawing should be avoided during the storage of samples
• Transport on dry ice

Advantages

• Advanced sequencing technologies including Illumina HiSeq 2500 and HiSeq 4000 instruments
• High throughput coverage: comprehensively detect microbial information in the ecological environment
• Fully explore the microbial diversity and dynamic changes in the samples
• Widely used for the analysis of microbial communities from the intestinal tract, soil, water, etc.
• Rich experience: have accomplished metatranscriptomic sequencing and analysis for microorganisms in various ecological environments, such as soil, sewage, silt, insect intestines, oral cavity, and feces

If you are looking for a company to help you characterize the specific expression profiles of the microbes using metatranscriptomic sequencing, please feel free to contact us for more details.

For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.