MIC and MBC Testing Service using Dilution Method
Services Samples Deliverables Case Studies Advantages Applications FAQs
Creative Biolabs' MIC & MBC Testing Services Employing Dilution Methodology: Your Partner in Antimicrobial Efficacy.
Determining the MIC and MBC – that's where we get the quantitative data on an antimicrobial's potency against bacterial targets. It's a cornerstone of our microbiological work. Addressing the challenges of precise antimicrobial compound effectiveness determination, and the consequent need for robust data in drug development and quality control, Creative Biolabs delivers meticulously executed MIC & MBC dilution assays. These services yield dependable results, facilitating accelerated research outcomes and ensuring product efficacy. Our services furnish clients with essential data for applications in drug development, fundamental research, and quality control. Request a Quote
Precision MIC & MBC Testing Services: Accurate Antimicrobial Susceptibility
Workflow
Details of Services
1. Sample Preparation
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Microorganism Preparation: A standardized suspension of the test microorganism is prepared and used to inoculate each antimicrobial dilution.
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Using appropriate quality control strains to validate the testing procedures.
2. Antimicrobial Preparation
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Preparing serial dilutions of the antimicrobial agents to cover a range of concentrations.
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Ensuring the accuracy and stability of the antimicrobial solutions.
3. MIC Testing (Broth or Agar Dilution)
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Performing serial dilutions of the antimicrobial agents in a liquid broth medium or on agar plates.
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Inoculating the diluted antimicrobial solutions with the standardized bacterial suspension.
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Incubation: Incubate the inoculated media under controlled conditions (temperature, time) to allow bacterial growth.
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MIC Determination: Identifying the lowest concentration of the antimicrobial agent that inhibits visible growth (MIC).
4. MBC Testing
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Subculturing: Taking samples from the MIC test dilutions.
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Incubation
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MBC Determination: Identifying the lowest concentration of the antimicrobial agent that results in a 99.9% reduction in viable bacterial colonies (MBC).
5. Data Analysis and Reporting
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Data Interpretation: Analyzing the MIC and MBC values to determine the susceptibility or resistance of the bacteria to the antimicrobial agents.
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Calculating MBC/MIC ratios to assess the bactericidal or bacteriostatic activity of the agents.
Including positive and negative controls in each test run to ensure the accuracy and reliability of the results.
6. Additional Potential Services
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Strain Selection
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Time-kill studies: These studies determine the rate of killing of bacteria by an antimicrobial agent over time.
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Resistance testing: Further investigation into mechanisms of resistance.
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Customized Testing: Tailoring the testing to meet specific client needs, such as testing specific antimicrobial agents or bacterial species.
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Types of Samples
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Pure bacterial cultures: Used in research and pharmaceutical development to test the efficacy of new antimicrobial agents, including probiotics.
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Environmental samples: Such as water, soil, and surface swabs, to assess bacterial contamination and the effectiveness of disinfectants.
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Food samples: To evaluate the presence of foodborne pathogens and the effectiveness of food preservatives.
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Pharmaceutical products: To verify the antimicrobial properties of drug formulations and compounds.
Ready to send samples.
Deliverables
The ultimate deliverable of MIC & MBC testing services is a comprehensive, clear, and actionable report that provides the client with the information they need to make informed decisions.
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Items
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Contents
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Report
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Accurate MIC and MBC Values for each tested antimicrobial agent-microorganism combination.
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A detailed description of the testing procedure, including specific strains, growth medium, and incubation conditions.
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Interpretation of results, including susceptibility/resistance classifications based on established guidelines (e.g., CLSI, EUCAST).
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Case Studies
Case 1: Determination of the MICs for Bacillus subtilis
Sample: Pure culture of Bacillus subtilis (Glycerol frozen form provided by the client.)
Antibiotics: 13 antibiotics, including Vancomycin, Chloramphenicol Clindamycin, Gentamycin Penicillin, etc. (Provided by Creative Biolabs.)
Download for Details
Case 2: MIC Testing for an API Against Standard Human Intestinal and Swine Pathogenic Bacteria
Sample: Compound (API powder provided by the client.)
Bacteria: 14 strains (Intestinal bacteria and Swine pathogens), provided by Creative Biolabs.
Download for Details
Service Brochure
Advantages
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Accurate, Reliable Results: Adherence to industry-standard protocols (e.g., CLSI) ensures a high degree of accuracy and reliability.
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Comprehensive Testing: Capability to test a broad spectrum of antimicrobial agents and microorganisms, including bacteria and fungi.
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Customized Solutions: We develop testing protocols that are highly customized, directly addressing the unique requirements of each client's research or product development goals.
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Expedited Turnaround Times: Timely delivery of results to accelerate client decision-making.
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Expert Support: Guidance and support from experienced microbiologists throughout the testing process.
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Established Methodology: Alignment of methods and results with well-established scientific literature, ensuring credibility.
Applications
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Microbiology, probiotics, infectious disease research.
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Novel antimicrobial drug development (Antimicrobial agents, such as antibiotics and antifungals.), evaluating drug potency, and ensuring clinical efficacy.
If you need solid MIC and MBC data to move your work forward, Creative Biolabs is here to deliver. We don't do cookie-cutter; we tailor our assays to the specifics of your project. We're talking quantitative results, the kind that drives informed decisions, whether you're refining a treatment protocol or pushing a drug candidate. So, let's hash out the details. Reach out, and we'll get down to the practicalities of how we can support your research.
MIC & MBC Testing Services FAQs
Why are MIC and MBC tests important?
If you want solid data on antimicrobial efficacy, you need MIC and MBC values. They're the bedrock for making informed calls on treatment protocols and drug development. We're here to help you figure out how these assays fit into your specific research.
What types of microorganisms can you test?
We can work with a wide range of microbial species – the usual bacterial and fungal suspects, of course, but also the more specialized ones, like those next-gen gut probiotics. If you're dealing with a particular organism, just shoot us a line, and we'll send over a comprehensive list of our testable species.
What is the typical turnaround time for results?
We're typically looking at a 2-4 weeks turnaround, but that's a rough estimate – study complexity can throw a wrench in that. For a solid timeline on your project, let's dig into the details.
Is data interpretation provided?
Yes, we deliver detailed reports that include data interpretation and analysis. Our experts are available to assist in results comprehension. Inquire now to discuss your data requirements.
Can testing protocols be customized?
We team up with you to hammer out testing strategies that are dead-on for your research or product development aims, Let's get down to the nitty-gritty of your project and map out the most effective approach, collaboratively.
Customer Reviews
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"Rigorous Testing: Creative Biolabs's MIC & MBC testing significantly improved the accuracy of our antimicrobial susceptibility testing, ensuring data reliability." Dr. Chr***pher, Research Scientist.
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"Fast Turnaround: The rapid turnaround time at Creative Biolabs for MIC & MBC testing greatly facilitated our drug development process, enabling timely decisions." Dr. El***beth, Pharmaceutical Researcher.
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"Reliable Results: The detailed reports and expert support from Creative Biolabs have enhanced our understanding of antimicrobial efficacy, crucial for our quality control." Mark***ael, Quality Control Manager.
"Reliable Solutions for Antibiotic Susceptibility Testing: MIC & MBC Services You Can Trust"
For Research Use Only. Not intended for use in
food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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