Clostridium sporogenes Spore Suspensions is a calibrated bacterial spore suspension suitable for direct inoculation of products or selection of substrates to monitor sterilization processes.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information
Product Overview
Clostridium sporogenes Spore Suspensions is a calibrated bacterial spore suspension suitable for direct inoculation of products or selection of substrates to monitor sterilization processes.
Target
Clostridium sporogenes
Size
5 mL per vial
Population
10^7 CFU/mL
Format
Suspensions
Diluent
Aqueous or 20% ethanol
Packaging
Penicillin bottle
Storage
2-8°C
Shelf Life
12 months from date of manufacture.
Applications
Used to validate sterilizers and monitor the effectiveness of the sterilization process.
FAQsPublished Data Customer Reviews Related Products
What is the primary application of Clostridium sporogenes spore suspension in research?
Clostridium sporogenes spore suspensions are primarily used for sterilization validation studies, anaerobic microbiology research, and as a surrogate in pathogenicity testing. The spores' high resistance to heat and chemical agents makes them ideal for testing sterilization processes and studying anaerobic bacteria behavior in various condition.
How should Clostridium sporogenes spore suspension be stored?
Clostridium sporogenes spore suspensions should be stored at 2-8°C to maintain viability and stability. Proper storage conditions are essential to ensure the spores' effectiveness in experimental applications and to prevent any degradation or loss of viability over time.
What are the safety precautions when handling Clostridium sporogenes spore suspension?
When handling Clostridium sporogenes spore suspensions, it is crucial to use appropriate personal protective equipment (PPE), including gloves and lab coats, and to work in a biosafety cabinet if possible. Although C. sporogenes is non-pathogenic, standard microbiological practices should be followed to prevent contamination and ensure safety.
Can Clostridium sporogenes spore suspension be used for comparative studies with Clostridium botulinum?
Yes, Clostridium sporogenes spore suspensions are often used as non-toxic surrogates for Clostridium botulinum in comparative studies. Due to their similar resistance profiles, C. sporogenes can be used to validate sterilization methods and study spore resistance mechanisms without the risks associated with C. botulinum.
How are Clostridium sporogenes spores quantified in the suspension?
The concentration of Clostridium sporogenes spores in a suspension is typically determined through direct spore counts using microscopy or by plating techniques that allow for the enumeration of colony-forming units (CFUs). Accurate quantification ensures reliable results in sterilization validation and microbiological studies.
Temperature values in meat and meat+blood mixture subjected to CaO action (Cº)
The study focuses on the efficacy of CaO (quicklime) in inactivating Clostridium sporogenes spores in meat waste. Pathogenic species like Clostridium perfringens and Clostridium botulinum in animal by-products pose significant health risks due to their potent toxins. The research evaluates the viability of Clostridium sporogenes, a surrogate for pathogenic clostridia, under different doses of CaO in poultry meat and meat mixed with swine blood. Two CaO doses, 50% and 70%, were tested for their sanitization effectiveness. Microbiological analysis showed that a 70% CaO dose completely inactivated Clostridium spores within 48 hours, achieving a peak temperature of 67°C and a pH above 12. The lower dose (50%) caused a gradual spore reduction, indicating that rapid alkalization was crucial for effective sanitization. The study demonstrates that CaO hygienization can significantly reduce health risks by inactivating spores in animal waste, making it a viable method for safe agricultural applications. These findings suggest that CaO treatment is effective in ensuring biosafety in the processing and utilization of meat waste, aligning with EU regulations for alternative waste disposal methods.
Bauza-Kaszewska, J., Paluszek, Z., et al. Viability of Clostridium sporogenes spores after CaO hygienization of meat waste. Annals of Agricultural and Environmental Medicine. 2014, 21(3).
Ideal for Pathogenicity Testing
Creative Biolabs' spore suspension has proven ideal for pathogenicity testing in our lab. Its high resistance to heat and chemicals makes it a superior choice compared to other products we've used. Highly recommended for anaerobic microbiology studies.
Reliable and Effective
This spore suspension is incredibly reliable for our anaerobic microbiology research. The high concentration and purity ensure consistent results in our experiments. Creative Biolabs has exceeded our expectations with this product.
Enhanced Sterilization Validation
Using Creative Biolabs' Clostridium sporogenes spore suspension has greatly improved our sterilization validation processes. Its stability and high spore count have provided more accurate and dependable results in our studies.
Superior Performance in Comparative Studies
The performance of Creative Biolabs' spore suspension in comparative studies with Clostridium botulinum has been outstanding. It serves as an excellent surrogate, ensuring safety and efficiency in our research without compromising results.
Unmatched Quality and Stability
Creative Biolabs offers unmatched quality and stability with their Clostridium sporogenes spore suspension. It has become an indispensable tool in our lab for various microbiological applications. Highly recommend this product for consistent and accurate research outcomes.
Click the button below to contact us or submit your feedback about this product.
