Clostridium sporogenes Spore Suspensions is a calibrated bacterial spore suspension suitable for direct inoculation of products or selection of substrates to monitor sterilization processes.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information | |
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Product Overview | Clostridium sporogenes Spore Suspensions is a calibrated bacterial spore suspension suitable for direct inoculation of products or selection of substrates to monitor sterilization processes. |
Target | Clostridium sporogenes |
Size | 5 mL per vial |
Population | 10^7 CFU/mL |
Format | Suspensions |
Diluent | Aqueous or 20% ethanol |
Packaging | Penicillin bottle |
Storage | 2-8°C |
Shelf Life | 12 months from date of manufacture. |
Applications | Used to validate sterilizers and monitor the effectiveness of the sterilization process. |
Clostridium sporogenes spore suspensions are primarily used for sterilization validation studies, anaerobic microbiology research, and as a surrogate in pathogenicity testing. The spores' high resistance to heat and chemical agents makes them ideal for testing sterilization processes and studying anaerobic bacteria behavior in various condition.
Clostridium sporogenes spore suspensions should be stored at 2-8°C to maintain viability and stability. Proper storage conditions are essential to ensure the spores' effectiveness in experimental applications and to prevent any degradation or loss of viability over time.
When handling Clostridium sporogenes spore suspensions, it is crucial to use appropriate personal protective equipment (PPE), including gloves and lab coats, and to work in a biosafety cabinet if possible. Although C. sporogenes is non-pathogenic, standard microbiological practices should be followed to prevent contamination and ensure safety.
Yes, Clostridium sporogenes spore suspensions are often used as non-toxic surrogates for Clostridium botulinum in comparative studies. Due to their similar resistance profiles, C. sporogenes can be used to validate sterilization methods and study spore resistance mechanisms without the risks associated with C. botulinum.
The concentration of Clostridium sporogenes spores in a suspension is typically determined through direct spore counts using microscopy or by plating techniques that allow for the enumeration of colony-forming units (CFUs). Accurate quantification ensures reliable results in sterilization validation and microbiological studies.
Temperature values in meat and meat+blood mixture subjected to CaO action (Cº)
The study focuses on the efficacy of CaO (quicklime) in inactivating Clostridium sporogenes spores in meat waste. Pathogenic species like Clostridium perfringens and Clostridium botulinum in animal by-products pose significant health risks due to their potent toxins. The research evaluates the viability of Clostridium sporogenes, a surrogate for pathogenic clostridia, under different doses of CaO in poultry meat and meat mixed with swine blood. Two CaO doses, 50% and 70%, were tested for their sanitization effectiveness. Microbiological analysis showed that a 70% CaO dose completely inactivated Clostridium spores within 48 hours, achieving a peak temperature of 67°C and a pH above 12. The lower dose (50%) caused a gradual spore reduction, indicating that rapid alkalization was crucial for effective sanitization. The study demonstrates that CaO hygienization can significantly reduce health risks by inactivating spores in animal waste, making it a viable method for safe agricultural applications. These findings suggest that CaO treatment is effective in ensuring biosafety in the processing and utilization of meat waste, aligning with EU regulations for alternative waste disposal methods.
Bauza-Kaszewska, J., Paluszek, Z., et al. Viability of Clostridium sporogenes spores after CaO hygienization of meat waste. Annals of Agricultural and Environmental Medicine. 2014, 21(3).
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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