Bacteroides fragilis was isolated from infant with diarrhea. It is an anaerobic, Gram-negative, pleomorphic to rod-shaped bacterium.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information
Product Overview
Bacteroides fragilis was isolated from infant with diarrhea. It is an anaerobic, Gram-negative, pleomorphic to rod-shaped bacterium.
Target
Bacteroides
Genus
Bacteroides
Strain Designation
20793-3
Application
Study and research
Type Strain
No
Culture Medium
Blood agar
Culture Conditions
37°C; Anaerobic
Source
Infant with diarrhea, Montana
Risk Group
2
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Bacteroides is a group of promising candidates that has attracted major attentions from scientists as model organisms or community for the investigations of the intestinal microbiota field, due to their powerful adaption characteristics in the host and especially the underlying benefits. Bacteroides is a genus of Gram-negative, obligate anaerobic bacteria. Bacteroides species are non endospore-forming bacilli, and may be either motile or nonmotile, depending on the species. Bacteroides; order is the most abundant group of Gram-negative bacteria which flourish in the human intestine at high densities of 109-1011 CFU per gram of feces, and constitutes over 30 species which are more closely related than members in other bacteria orders. And, Bacteroides is considered as one of the major genera of the core microbiota module, of which species with relative abundance of over 1% refers to B. uniformis, B. vulgatus, B. caccae, and B. thetaiotaomicron. In addition to the unique physiological characteristics and the special ecological dynamics, Bacteroides species are under investigations for their underlying beneficial dialogues with the host. Bacteroides species to be considered as next-generation probiotics, and the application of which in prevention or intervention in dysbiosis of gut microbiota and the associated disorders requires further investigations in the modulatory mechanisms and safety evaluation. Bacteroides is one of the dominant contributors of SCFA among the intestinal commensals, mostly in the form of acetate and propionate. Accumulated acetate blocks the transportation of toxins between gut lumen and blood, while propionate is capable of inducing the apoptosis of human colon carcinoma cells, and thus avoids the formation of tumors. Moreover, Vitamin K, which is mainly synthesized by gut microbiota candidates such as Bacteroides, can help prevent or treat osteoporosis by increasing bone mineral density.
Alternative Names
Bacteroides fragilis; 20793-3
FAQsPublished Data Customer Reviews Related Products
What are the key genetic features of Bacteroides fragilis ?
The strain has been sequenced, contributing valuable genetic data for understanding its capabilities and interactions within the gut microbiota. This strain, like others within the Bacteroides genus, is not endospore-forming and can be either motile or nonmotile.
How should Bacteroides fragilis be stored to maintain viability?
For optimal viability, Bacteroides fragilis should be stored under anaerobic conditions at -80°C. It's important to use appropriate cryoprotectants such as glycerol to prevent ice crystal formation, which can damage the cells.
How should waste materials containing Bacteroides fragilis be disposed of?
Waste materials containing Bacteroides fragilis should be treated as biohazardous. This includes autoclaving all cultures, media, and other materials that have come into contact with the bacteria before disposal to prevent environmental contamination and ensure laboratory safety.
Can Bacteroides fragilis be used in animal models?
While Bacteroides fragilis is primarily intended for in vitro research, using this strain in animal models requires specific considerations such as ensuring the strain's compatibility with the host animal and meeting ethical guidelines for animal research.
How is Bacteroides fragilis used in probiotic research?
As next-generation probiotics, strains like Bacteroides fragilis are explored for their potential to modulate the immune system and promote gut health, thanks to their ability to interact closely with the host's biological systems.
Diversity of B. fragilis ETBF and NTBF strains
The research detailed in the document examines the genomic diversity of enterotoxigenic strains of Bacteroides fragilis, specifically focusing on the strain 20793-3 among others. The study underscores that Bacteroides fragilis strains exhibit significant genomic variation, especially in genes located in the Bft pathogenicity island (BFT PAI) and associated CTn86 conjugative transposon. These genes are crucial as they code for the toxin fragilysin (Bft), which has been linked to both diarrheal diseases and colorectal cancer due to its impact on cellular processes like E-cadherin cleavage. Experimentally, it was shown that the enterotoxigenic strains (ETBF), including 20793-3, have a heightened ability to form biofilms compared to non-toxigenic strains (NTBF). This trait potentially facilitates the persistence and pathogenicity of these bacteria within the host. Despite the genomic diversity, these strains share common phenotypic traits, such as the secretion of Bft and the ability to form biofilms, which could be critical in promoting colorectal tumorigenesis. This study not only advances the understanding of Bacteroides fragilis variability and pathogenicity but also raises important considerations for therapeutic strategies targeting microbial factors in colorectal cancer.
Pierce, J. V., & Bernstein, H. D. Genomic diversity of enterotoxigenic strains of Bacteroides fragilis. PLoS One. 2016, 11(6): e0158171. Distributed under Open Access license CC0 1.0 Universal, without modification.
Exceptional Genetic Stability
Creative Biolabs' Bacteroides fragilis; 20793-3 strain has exceptional genetic stability, which is crucial for our long-term pharmaceutical studies.
Unsurpassed in Research Efficiency
Creative Biolabs' Bacteroides fragilis; 20793-3 has unsurpassed efficiency in our metabolic studies, providing reliable results faster than competitor products.
Trusted for Reproducibility
With Bacteroides fragilis; 20793-3, Creative Biolabs has provided a product we trust for its reproducibility and robustness in clinical simulation studies.
Superior Quality
Creative Biolabs' Bacteroides fragilis; 20793-3 is of superior quality, enabling pioneering work in our pathogen interaction research.
Pivotal for Innovative Research
Using Bacteroides fragilis; 20793-3 from Creative Biolabs has been pivotal in our innovative approaches to studying host-microbe interactions, due to its exceptional purity and growth properties.
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