Bacteroides fragilis was isolated from human perianal abscess. It is an anaerobic, Gram-negative, pleomorphic to rod-shaped bacterium.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information
Product Overview
Bacteroides fragilis was isolated from human perianal abscess. It is an anaerobic, Gram-negative, pleomorphic to rod-shaped bacterium.
Target
Bacteroides
Genus
Bacteroides
Application
Study and research
Culture Medium
Blood agar
Culture Conditions
37°C; Anaerobic
Source
Human perianal abscess
Risk Group
2
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Bacteroides is a group of promising candidates that has attracted major attentions from scientists as model organisms or community for the investigations of the intestinal microbiota field, due to their powerful adaption characteristics in the host and especially the underlying benefits. Bacteroides is a genus of Gram-negative, obligate anaerobic bacteria. Bacteroides species are non endospore-forming bacilli, and may be either motile or nonmotile, depending on the species. Bacteroides; order is the most abundant group of Gram-negative bacteria which flourish in the human intestine at high densities of 109-1011 CFU per gram of feces, and constitutes over 30 species which are more closely related than members in other bacteria orders. And, Bacteroides is considered as one of the major genera of the core microbiota module, of which species with relative abundance of over 1% refers to B. uniformis, B. vulgatus, B. caccae, and B. thetaiotaomicron. In addition to the unique physiological characteristics and the special ecological dynamics, Bacteroides species are under investigations for their underlying beneficial dialogues with the host. Bacteroides species to be considered as next-generation probiotics, and the application of which in prevention or intervention in dysbiosis of gut microbiota and the associated disorders requires further investigations in the modulatory mechanisms and safety evaluation. Bacteroides is one of the dominant contributors of SCFA among the intestinal commensals, mostly in the form of acetate and propionate. Accumulated acetate blocks the transportation of toxins between gut lumen and blood, while propionate is capable of inducing the apoptosis of human colon carcinoma cells, and thus avoids the formation of tumors. Moreover, Vitamin K, which is mainly synthesized by gut microbiota candidates such as Bacteroides, can help prevent or treat osteoporosis by increasing bone mineral density.
Alternative Names
Bacteroides fragilis; 36902
FAQs Customer Reviews Related Products
How should Bacteroides fragilis be prepared for use in experimental assays?
After thawing, inoculate Bacteroides fragilis into pre-reduced anaerobic media and incubate at 37°C until the culture reaches the desired optical density. This preparation ensures the bacteria are in optimal condition for experimental assays.
What methods can confirm the purity of Bacteroides fragilis cultures?
Purity can be confirmed by streaking cultures onto selective agar plates and checking for uniform colony morphology. Additionally, PCR-based methods can be used to verify the genetic identity and purity of the strain.
What medium is most effective for culturing Bacteroides fragilis?
The most effective medium for culturing Bacteroides fragilis is Brain Heart Infusion (BHI) broth supplemented with hemin and vitamin K. This medium supports robust growth and maintains the physiological properties of the strain.
What techniques are recommended for verifying the identity of Bacteroides fragilis in research studies?
The identity of Bacteroides fragilis can be verified using 16S rRNA sequencing and strain-specific PCR assays. These techniques ensure the genetic fidelity of the strain and confirm its suitability for research applications.
Effortless Revitalization
Revitalizing Bacteroides fragilis; 36902 from frozen stocks is effortless and efficient, saving valuable time in our research procedures. This ease of use is highly beneficial.
Consistent Culturing Performance Bacteroides fragilis; 36902 consistently performs well in anaerobic cultures, providing reliable growth for our host-pathogen interaction experiments. Its performance is a significant advantage in our research.
Reliable Experimental Data
Using Bacteroides fragilis; 36902 has provided us with reliable data for our gut microbiota research. Its dependable performance is critical for obtaining accurate results.
Excellent Handling Properties Bacteroides fragilis; 36902 is easy to handle, even under strict anaerobic conditions, making it a valuable asset in our laboratory. Its handling properties streamline our experimental workflows.
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