Lactobacillus crispatus is a common, rod-shaped species of genus Lactobacillus and is a hydrogen peroxide producing beneficial microbiota species that was isolated from female genital tract.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information
Product Overview
Lactobacillus crispatus is a common, rod-shaped species of genus Lactobacillus and is a hydrogen peroxide producing beneficial microbiota species that was isolated from female genital tract.
Target
Lactobacillus
Genus
Lactobacillus
Strain Designation
B471
Application
Study and research
Type Strain
No
Culture Medium
MRS
Culture Conditions
37°C; Anaerobic
Source
Female genital tract
Characteristics
Gram-positive bacteria, no flagellum, no spores. The colonies were white, irregular edge, flake, central convex, not liquefied gelatin. It can use mannose, galactose, lactose, glucose, maltose, honey disaccharide, raffinose, ribose, sucrose, trehalose, fructose.
Risk Group
1
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Lactobacillus species are normal flora of the human mouth, gastrointestinal tract, and female genital tract, where they produce lactic acid and competitively inhibit pathogenic organisms. Lactobacillus are small, slender, nonmotile, Gram-positive bacilli in chains. Immature colonies appear smooth, convex, and translucent. Some species of Lactobacillus used commonly as probiotics. They are most commonly used for diarrhea, including infectious diarrhea and diarrhea in people taking antibiotics. Some people also use lactobacillus for general digestion problems, irritable bowel syndrome; IBS, colic in infants, and many other conditions that involve the stomach and bowel.
Alternative Names
Lactobacillus crispatus
FAQs Customer Reviews Related Products
How should Lactobacillus crispatus be prepared for in vitro probiotic studies?
Lactobacillus crispatus should be cultured in MRS broth under anaerobic conditions, typically at 37°C. After reaching the desired growth phase, cells can be harvested, washed with saline, and resuspended in sterile media or buffer for use. It's crucial to maintain sterile conditions to ensure the integrity of the bacterial sample.
How is the viability of Lactobacillus crispatus monitored during experiments?
The viability of Lactobacillus crispatus can be monitored using colony-forming unit (CFU) assays. Researchers plate diluted samples on MRS agar and incubate anaerobically. The resulting colonies are counted to determine the number of viable bacteria present at different stages of the experiment.
What is the recommended storage condition for maintaining the viability of Lactobacillus crispatus?
For long-term storage, Lactobacillus crispatus should be stored at -80°C in cryoprotectant solutions, such as glycerol, to ensure viability. Short-term storage can be done at 4°C if used within a few days. These conditions preserve its integrity and functionality for future experiments.
Can Lactobacillus crispatus be genetically manipulated for experimental purposes?
Yes, genetic manipulation techniques such as CRISPR/Cas9 and plasmid transformation have been used in Lactobacillus crispatus. These methods allow researchers to explore gene function and engineer strains for specific probiotic properties.
How is the viability of Lactobacillus crispatus determined during experiments?
Viability can be assessed through colony-forming unit (CFU) counts, optical density measurements at 600 nm (OD600), or fluorescent dyes that differentiate live from dead cells. These methods help confirm the strain's functionality throughout the experimental process.
Excellent for Microbiome Drug Research
The Lactobacillus crispatus strain significantly contributed to my microbiome drug development studies. Its stability and purity were essential in generating consistent and reproducible results.
Key in Vaginal Microbiota Analysis Lactobacillus crispatus was crucial for my vaginal microbiota studies, offering excellent data accuracy and reliable bacterial interaction insights during drug discovery processes.
Ideal for Host-Microbe Interaction Studies
The Lactobacillus crispatus strain performed exceptionally well in my host-microbe interaction models. It provided valuable insights into immune modulation during therapeutic research.
Perfect for Biofilm Research Lactobacillus crispatus was indispensable in my biofilm formation studies. Its ability to thrive under experimental conditions made it an ideal candidate for exploring bacterial behavior in complex environments.
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