Lactobacillus casei is a Gram-positive, facultatively anaerobic, non-motile and non-spore-forming, rod-shaped bacterium
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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LBST-115FG |
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Product Information | |
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Product Overview | Lactobacillus casei is a Gram-positive, facultatively anaerobic, non-motile and non-spore-forming, rod-shaped bacterium |
Target | Lactobacillus |
Genus | Lactobacillus |
Strain Designation | Y5-2b |
Application | Study and research |
Type Strain | No |
Culture Medium | MRS |
Culture Conditions | 37°C; Aerobiotic |
Characteristics | It can ferment glucose, fructose, cellulose-disaccharide, galactose, triose, sucrose, maltose, mannitol, mannose, trehalose. Gram-positive, rod-shaped, no buds, round colony, facultative anaerobic, it can grow at 15°C. High proteolytic activity. |
Risk Group | 1 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
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Introduction | Lactobacillus species are normal flora of the human mouth, gastrointestinal tract, and female genital tract, where they produce lactic acid and competitively inhibit pathogenic organisms. Lactobacillus are small, slender, nonmotile, Gram-positive bacilli in chains. Immature colonies appear smooth, convex, and translucent. Some species of Lactobacillus used commonly as probiotics. They are most commonly used for diarrhea, including infectious diarrhea and diarrhea in people taking antibiotics. Some people also use lactobacillus for general digestion problems, irritable bowel syndrome; IBS, colic in infants, and many other conditions that involve the stomach and bowel. |
Alternative Names | Lactobacillus casei; Y5-2b |
Lactobacillus casei; Y5-2b should be cultured in MRS broth at 37°C under anaerobic conditions. Ensuring these specific conditions supports optimal bacterial growth and viability, which is essential for accurate research outcomes.
Viability of Lactobacillus casei; Y5-2b can be assessed using plate counts, flow cytometry, and qPCR. These methods help determine the number of live bacteria, ensuring the efficacy of probiotic formulations and research applications.
Lactobacillus casei; Y5-2b can be added to in vitro gut models by preparing a standardized bacterial suspension. It is crucial to monitor the concentration and environmental conditions to accurately simulate gut interactions and study probiotic effects.
To determine the growth curve of Lactobacillus casei; Y5-2b, measure the optical density (OD600) of the culture at regular intervals. Plot OD600 against time to visualize the lag, exponential, and stationary phases, providing insights into the bacterial growth dynamics.
For adhesion studies, prepare a suspension of Lactobacillus casei; Y5-2b and incubate it with epithelial cells. Measure the adherence by counting colony-forming units (CFUs) or using fluorescence microscopy to observe the interaction.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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