Pediococcus pentosaceus was isolated from fermented milk. They are coccus shaped microbes, Gram-positive, non-motile, non-spore forming, and are categorized as a "lactic acid bacteria". Pediococcus pentosaceus are able to grow in pH values between 4.5 and 8.0. Pediococcus are unique in that they form tetrads.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information | |
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Product Overview | Pediococcus pentosaceus was isolated from fermented milk. They are coccus shaped microbes, Gram-positive, non-motile, non-spore forming, and are categorized as a "lactic acid bacteria". Pediococcus pentosaceus are able to grow in pH values between 4.5 and 8.0. Pediococcus are unique in that they form tetrads. |
Target | Pediococcus |
Genus | Pediococcus |
Strain Designation | 32 |
Application | Study and research |
Type Strain | No |
Culture Medium | MRS |
Culture Conditions | 30°C; Facultative anaerobic |
Source | Fermented milk |
Risk Group | 1 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
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Introduction | Pediococcus is a genus of Gram-positive lactic acid bacteria, belonging to the family of Lactobacillacea. The genus Pediococcus consists of the following species: P. acidilactici, P. pentosaceus, P. damnosus, P. parvulus, P. inopinatus, P. halophilus, P. dextrinicus, and P. urinaeequi. Pediococcus spp. cells are spherical and arranged in tetrads. They are facultative anaerobes, non motile and non sporulating. The known pediocins from P. acidilactici, P. pentosaceus and P. damnosus strains are mostly small, hydrophobic proteins. Food application of pediocins can provide a good alternative means in protecting food against foodborne pathogens. Being mild antimicrobials, pediocins are also expected in the future to find more applications in both human and veterinary medicine. P. pentosaceus, a promising strain of lactic acid bacteria; LAB, is gradually attracting attention, leading to a rapid increase in experimental research. |
Alternative Names | Pediococcus pentosaceus; 32 |
Pediococcus pentosaceus; 32 can be administered to in vivo models via oral gavage, ensuring precise dosage and delivery. This method allows researchers to study its effects on gut microbiota composition and host health in a controlled experimental environment.
For in vitro assays, prepare Pediococcus pentosaceus; 32 by culturing it in an anaerobic chamber with a suitable growth medium until it reaches the desired optical density. Ensure that all equipment and media are sterilized to prevent contamination.
The presence of Pediococcus pentosaceus; 32 can be confirmed using molecular techniques such as 16S rRNA gene sequencing or species-specific PCR assays. These methods provide accurate identification and quantification of the bacterium in various samples.
The effects on immune responses can be studied by co-culturing Pediococcus pentosaceus; 32 with immune cells and measuring cytokine production using ELISA. Flow cytometry can also be employed to analyze changes in immune cell populations, providing insights into the bacterium's immunomodulatory effects.
Assess stress tolerance by exposing Pediococcus pentosaceus; 32 to different stress conditions, such as variations in pH, temperature, and osmotic pressure, and measuring survival rates using plate counts or flow cytometry. These experiments help determine the robustness of the bacterium under various environmental conditions.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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