Bifidobacterium adolescentis is a Gram-positive organism, containing one cell membrane, and is not mobile. They are normal inhabitants of healthy human and animal intestinal tracts. Colonization of Bifidobacterium adolescentis in the gut occurs immediately after birth.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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LBST-059FG |
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Product Information | |
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Product Overview | Bifidobacterium adolescentis is a Gram-positive organism, containing one cell membrane, and is not mobile. They are normal inhabitants of healthy human and animal intestinal tracts. Colonization of Bifidobacterium adolescentis in the gut occurs immediately after birth. |
Target | Bifidobacterium |
Genus | Bifidobacterium |
Application | Study and research |
Type Strain | No |
Culture Medium | BBL |
Culture Conditions | 37°C; Anaerobic |
Source | Human |
Risk Group | 1 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
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Introduction | Bifidobacterium are Gram-positive, heterofermentative, anaerobic bacteria with a distinctive bifid; i.e. Y shape after which they are named. They wre originally isolated from the faeces of breast-fed infants, members of the genus Bifidobacterium are commonly found in the GI tract of mammals. In humans, Bifidobacterium resides within the GI tract, from birth to old age. Disturbances in the microbiota are linked to an ever-growing number of immune-linked disease states including IBD, atopic allergy, arthritis, and obesity. Therefore, there is a significant interest in treating these diseases through microbial or "probiotic" supplementation of patients, including Bifidobacterium. Data from mouse models and clinical trials indicate that Bifidobacterium may have beneficial effects for treating and preventing immune-linked diseases, including gut-associated and systemic conditions. Bifidobacterium have been commercially exploited as probiotic agents due to their associated health benefits and GRAS; Generally Recognised As Safe status. |
Alternative Names | Bifidobacterium adolescentis; 186535 |
For in vitro experiments, Bifidobacterium adolescentis should be rehydrated from its lyophilized form using an appropriate anaerobic growth medium. The culture should then be incubated under anaerobic conditions at 37°C until the desired cell density is reached. Proper preparation is crucial for maintaining bacterial viability and experimental integrity
To ensure the long-term viability of Bifidobacterium adolescentis, it should be stored at -80°C in cryoprotectant media. Avoid repeated freeze-thaw cycles, as they can significantly reduce bacterial viability. Proper storage conditions are essential for preserving the strain's functionality in research applications.
The recommended concentration for animal studies varies, but typically ranges from 10^7 to 10^9 colony-forming units (CFU) per gram of diet or per milliliter of suspension. The specific dosage should be adjusted based on the experimental design and the animal model used to achieve optimal results.
Quantification of Bifidobacterium adolescentis can be performed using plate counting methods on selective media, flow cytometry, or quantitative PCR. Each method has its advantages, with plate counting providing viable cell counts and PCR offering precise quantification of bacterial DNA.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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