Strict Anaerobe Culturomics Services for Next-Generation Probiotics

We utilize strictly validated, pre-reduced anaerobic transport media and specialized gas-tight containers designed specifically for extremely sensitive obligate anaerobes. Upon arrival at our facility, samples are immediately transferred into our high-performance anaerobic workstations equipped with airlocks and mixed gas atmospheres, ensuring that the entire workflow maintains a virtually zero-oxygen environment from end to end.

Absolutely. Because many Next-Generation Probiotics possess unique nutritional and redox requirements, our culturomics team frequently develops custom complex media. We empirically optimize a variety of carbon sources, nitrogen complexes, specific growth factors (such as hemin or vitamins), and targeted reducing agents (like L-cysteine or sodium thioglycolate) to create the ideal physiological niche that maximizes the recovery and viability of uncharacterized strains.

Ensuring absolute purity is critical. We confirm strain purity through rigorous visual inspection of colony morphology combined with repeated anaerobic sub-culturing. Following isolation, identity confirmation options, such as 16S rRNA gene sequencing or Whole Genome Sequencing, are available upon request to meet your specific research needs.

Strict anaerobes like F. prausnitzii often suffer viability losses upon freezing and thawing. We screen tailored cryoprotectant formulations—integrating various concentrations of glycerol, skim milk, and specific antioxidants—to protect the cellular membrane from ice crystal damage and oxidative shock. We then perform post-thaw recovery rate analyses to provide you with reliable preservation matrix and storage recommendations.