Ruminococcus gnavus was isolated from human faeces.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
CAT | Size | Price | Quantity | |
---|---|---|---|---|
LBSX-0522-GF68 |
|
Product Information | |
---|---|
Product Overview | Ruminococcus gnavus was isolated from human faeces. |
Target | Ruminococcus |
Genus | Ruminococcus |
Strain Designation | VPI C7-9 |
Application | Study and research |
Type Strain | Yes |
Culture Medium | Columbia blood agar |
Culture Conditions | 37°C; Anaerobic |
Source | Human faeces |
Risk Group | 1 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
---|---|
Introduction | Ruminococcus is a genus of bacteria in the class Clostridia. They are anaerobic, Gram-positive gut microbes. One or more species in this genus are found in significant numbers in the human gut microbiota. |
Alternative Names | Ruminococcus gnavus; 29149 |
Ruminococcus gnavus should be stored under anaerobic conditions to maintain viability. Use sterile butyl rubber stoppers on test tubes to retain an anaerobic gas headspace, and store pre-reduced media properly to avoid oxidation.
Freshly prepared pre-reduced media is recommended. The use of a color indicator like resazurin in the media helps ensure anaerobic conditions. Pink coloration indicates oxidation, suggesting the media should be discarded.
Ruminococcus gnavus thrives in strictly anaerobic conditions with a temperature range of 37°C. It is important to monitor and maintain these conditions to ensure robust bacterial growth.
Whole-genome sequencing is commonly used to verify the identity of Ruminococcus gnavus. This ensures the accuracy and reliability of the bacterial strain being used in research.
Key parameters include growth rate, gene expression profiles, metabolic activity, and interaction with mucin glycans. These metrics help understand the bacterial role in gut health and disease.
Analysis of PSA-NCAM-positive cells in the SGZ of Rg-mice and GF mice
The research explored the influence of Ruminococcus gnavus 29149 on gut-brain communication, focusing on its impact on mucin glycosylation, metabolite production, and neurogenesis in gnotobiotic mice. Mice monocolonized with R. gnavus showed significant alterations in sialic acid derivatives in the cecum, blood, and brain, with decreased sialylation in intestinal mucins compared to germ-free mice. Notably, metabolites associated with brain function, such as tryptamine and indolacetate, were elevated in the cecal content of the R. gnavus-colonized mice. Additionally, these mice displayed a significant reduction in PSA-NCAM immunoreactive granule cells in the dentate gyrus, coupled with increased phagocytic microglia, indicating enhanced neurogenesis and synaptic plasticity. Behavioral tests suggested an improvement in spatial working memory but no changes in other cognitive functions. This study provides insights into how R. gnavus-derived metabolites may regulate brain function by modulating granule cell development and synaptic plasticity, highlighting the potential role of gut microbiota in neurological health.
Coletto, E., Latousakis, D., et al. The role of the mucin-glycan foraging Ruminococcus gnavus in the communication between the gut and the brain. Gut Microbes. 2022, 14(1): 2073784.
Click the button below to contact us or submit your feedback about this product.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Copyright © 2024 Creative Biolabs. All Rights Reserved.