Prevotella hominis cells are Gram-negative, non-spore-forming, nonmotile rods and strictly anaerobic. Colonies are approximately 0.5-1mm in diameter, slightly translucent, circular, entire, slightly convex and smooth after 2 days at 37 °C on modified GAM agar plates under anaerobic conditions.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information
Product Overview
Prevotella hominis cells are Gram-negative, non-spore-forming, nonmotile rods and strictly anaerobic. Colonies are approximately 0.5-1mm in diameter, slightly translucent, circular, entire, slightly convex and smooth after 2 days at 37 °C on modified GAM agar plates under anaerobic conditions.
Target
Prevotella
Genus
Prevotella
Application
Study and research
Culture Medium
GAM
Culture Conditions
37°C; Anaerobic
Source
Human feces, Taiwan
Risk Group
2
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Prevotella is a genus of anaerobic Gram-negative bacteria of the Bacteroidetes phylum, which also includes theclinically important genera Bacteroides and Porphyromonas. Prevotella strains are classically considered commensal bacteria due to their extensive presence inthe healthy human body and their rare involvement ininfections. Prevotella spp. are members of the oral, vaginal, and gut microbiota and are often recovered from anaerobic infections of the respiratory tract. Prevotella spp. predominate in periodontal disease and periodontal abscesses. Regarding Prevotella, studies focused on the associations between the diverse species, genome and habitats with dietary patterns, health and disease. Evidence revealed beneficial effects of some Prevotella strains in the gut such as improving CVD risk factor profile and glucose metabolism. Most studies reported that vegetarians harbour higher numbers of Prevotella species as well as a high Prevotella/Bacteroides ratio compared with non-vegetarians; omnivores. Nevertheless, there is a need for more studies in humans to ascertain a causal and potential disease-triggering role for Prevotella and more in-depth metagenomic studies are needed in order to reveal the health- or disease-modulating properties.
Alternative Names
Prevotella hominis Liou et al.
FAQs Customer Reviews Related Products
What is the recommended medium for culturing Prevotella hominis Liou et al.?
Prevotella hominis should be cultured using Brain Heart Infusion (BHI) broth or agar supplemented with hemin and vitamin K1. This medium provides the necessary nutrients and conditions to support the growth and viability of this anaerobic bacterium.
How should Prevotella hominis be revived from a frozen state?
To revive Prevotella hominis from a frozen state, rapidly thaw the vial in a 37°C water bath and immediately transfer the contents to pre-reduced anaerobic media. Incubate under anaerobic conditions at 37°C to ensure optimal recovery and growth.
What methods can be used to confirm the identity of Prevotella hominis in a sample?
Confirm the identity of Prevotella hominis using molecular techniques such as PCR with species-specific primers. Additionally, sequencing of the 16S rRNA gene can provide accurate identification. These methods ensure precise detection and validation of the strain.
How can Prevotella hominis be quantified in gut microbiota studies?
Quantification of Prevotella hominis in gut microbiota studies can be achieved using real-time PCR with species-specific primers. This technique allows precise detection and quantification of Prevotella hominis within complex microbial communities, facilitating detailed population analysis.
What assays are commonly used to evaluate the functional impact of Prevotella hominis on gut health?
Common assays include gut barrier integrity tests using transepithelial electrical resistance (TEER), cytokine profiling for immune response analysis, and microbial profiling using 16S rRNA sequencing. These assays help in understanding the role of Prevotella hominis in gut microbiota and health.
Outstanding Culture Viability
The culture viability of Creative Biolabs' Prevotella hominis is exceptional. The strain revived quickly from frozen storage, ensuring reliable and consistent growth for our gut microbiota studies.
Reliable for Molecular Identification
The molecular identification of Prevotella hominis from Creative Biolabs was precise and straightforward. PCR and sequencing confirmed its identity, providing confidence in our microbiome profiling studies.
Consistent Results in Functional Assays
Using Creative Biolabs' Prevotella hominis, we achieved consistent results in gut barrier integrity and cytokine profiling assays. This strain has been crucial in studying the impact of gut bacteria on host immune responses.
High-Quality Storage Stability
The storage stability of Creative Biolabs' Prevotella hominis is impressive. The strain remained viable after long-term storage at -80°C, ensuring reliable performance in subsequent experiments.
Effective for Microbial Interaction Studies
The Prevotella hominis strain from Creative Biolabs was highly effective in our microbial interaction studies. Its consistent performance allowed us to investigate complex interactions within the gut microbiota.
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