Lactobacillus casei is a Gram-positive, facultatively anaerobic, non-motile and non-spore-forming, rod-shaped bacterium that was isolated from dairy products.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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LBST-116FG |
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Product Information | |
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Product Overview | Lactobacillus casei is a Gram-positive, facultatively anaerobic, non-motile and non-spore-forming, rod-shaped bacterium that was isolated from dairy products. |
Target | Lactobacillus |
Genus | Lactobacillus |
Strain Designation | C27 |
Application | Study and research |
Type Strain | No |
Culture Medium | JCM13 |
Culture Conditions | 30°C; Aerobiotic |
Source | Dairy products |
Risk Group | 1 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
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Introduction | Lactobacillus species are normal flora of the human mouth, gastrointestinal tract, and female genital tract, where they produce lactic acid and competitively inhibit pathogenic organisms. Lactobacillus are small, slender, nonmotile, Gram-positive bacilli in chains. Immature colonies appear smooth, convex, and translucent. Some species of Lactobacillus used commonly as probiotics. They are most commonly used for diarrhea, including infectious diarrhea and diarrhea in people taking antibiotics. Some people also use lactobacillus for general digestion problems, irritable bowel syndrome; IBS, colic in infants, and many other conditions that involve the stomach and bowel. |
Alternative Names | Lactobacillus casei; C27 |
Lactobacillus casei; C27 can be prepared by cultivating the bacterial strain in an appropriate growth medium, usually MRS broth or agar, under anaerobic or microaerophilic conditions. The bacterial cells are then collected by centrifugation, washed, and resuspended in buffer or media for use in experiments. Maintaining viability and consistency in preparation is critical for reproducible results.
The optimal concentration of Lactobacillus casei; C27 for probiotic studies typically ranges between 10^6 to 10^9 CFU/mL. However, the exact dosage should be determined based on the specific research objective, whether it's for gut microbiota modulation, immune response studies, or other applications. Higher or lower concentrations might be needed depending on experimental design.
Yes, Lactobacillus casei; C27 can be co-cultured with other bacterial strains in microbiome studies. However, optimizing the media and growth conditions for all bacterial species involved is crucial to ensure that Lactobacillus casei grows effectively without being outcompeted. Careful monitoring of the growth kinetics is recommended.
Quantification can be achieved using quantitative PCR (qPCR) with strain-specific primers to detect Lactobacillus casei; C27 in mixed communities. Additionally, fluorescent in situ hybridization (FISH) or flow cytometry with strain-specific markers can help differentiate it from other microbes.
Lactobacillus casei; C27 can be administered to animal models via oral gavage, incorporation into feed, or addition to drinking water. The delivery method should be tailored to the study design, and researchers should ensure accurate dosage calculation and a suitable vehicle for bacteria administration to maintain bacterial viability throughout the process.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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