Bacillus licheniformis is a Gram-positive, mesophilic bacterium. Its optimal growth temperature is around 50°C. It was isolated from flour.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information
Product Overview
Bacillus licheniformis is a Gram-positive, mesophilic bacterium. Its optimal growth temperature is around 50°C. It was isolated from flour.
Target
Bacillus
Genus
Bacillus
Strain Designation
NRS 712
Application
Study and research
Type Strain
No
Culture Medium
Nutrient Agar
Culture Conditions
37°C, Facultative anaerobic
Source
Flour
Risk Group
1
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Bacillus is a genus of Gram-positive, rod-shaped bacteria, a member of the phylum Bacillota. Bacillus species can be either obligate aerobes which are dependent on oxygen, or facultative anaerobes which can survive in the absence of oxygen.
Alternative Names
B. licheniformis
FAQs Customer Reviews Related Products
What are the specific nutrient requirements for culturing Bacillus licheniformis?
Bacillus licheniformis thrives in nutrient-rich media such as LB broth or TSB. Supplementing with trace elements like magnesium and manganese can enhance growth and metabolic activity, which is crucial for experimental studies.
How is the enzymatic activity of Bacillus licheniformis typically measured?
The enzymatic activity can be measured using spectrophotometric assays that monitor the release of products from specific substrates. For instance, protease activity is often assessed by hydrolyzing casein and measuring the resulting peptides.
What procedures should be followed for the lyophilization of Bacillus licheniformis?
Lyophilization involves freezing Bacillus licheniformis cultures at -80°C, followed by vacuum drying. Adding cryoprotectants like glycerol before freezing can help maintain cell viability during the lyophilization process.
What are the common methods for genetic transformation of Bacillus licheniformis?
Common methods include electroporation and heat shock transformation. These techniques require the preparation of competent cells and the use of plasmid DNA or other vectors to introduce new genetic material into the bacteria.
How should Bacillus licheniformis cultures be handled to prevent contamination?
To prevent contamination, work in a sterile environment using aseptic techniques. Sterilize all equipment and media before use, and regularly monitor cultures for signs of contamination such as unexpected growth or changes in turbidity.
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