Expert Antifungal Activity Testing Solution
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As research scientists dedicated to advancing therapeutic strategies against fungal pathogens, we at Creative Biolabs offer a suite of meticulously validated antifungal testing solutions. Driven by our scientific rigor and a shared goal of combating fungal diseases, Creative Biolabs delivers high-precision, reliable antifungal testing with efficient timelines. This directly fuels pivotal stages of your antifungal research and development pipelines. Acknowledging the multifaceted challenges of drug discovery and preclinical studies, we offer a comprehensive portfolio of methodologies and the adaptability necessary to craft solutions precisely suited to your distinct experimental demands. Request a quote
Reliable Antifungal Screening & Potency Testing Services
Workflow
Preparation of Fungal Culture
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Cultivate fungi under appropriate medium and culture conditions.
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Collect fungal spores or cells, adjust the cell density, and prepare standard inoculates.
Fungal sample types
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Types
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Species
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Yeasts
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Candida species: C. albicans, C. glabrata, C. krusei, C. parapsilosis,C. tropicalis, and the emerging multidrug-resistant C. auris.
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Cryptococcus species: C. neoformans.
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Molds (Filamentous Fungi)
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Aspergillus species: A. fumigatus, A. flavus, A. niger, A. terreus.
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Mucorales: Rhizopus, Mucor, Lichtheimia.
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Fusarium species: Fusarium solani, Fusarium oxysporum.
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Dermatophytes: Trichophyton (Trichophyton rubrum, Trichophyton mentagrophytes, Trichophyton interdigitale, Trichophyton interdigitale), Microsporum, Epidermophyton.
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et al.
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Environmental and Industrial Fungi
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Certain tests might include common environmental molds like Penicillium and Aspergillus niger (non-pathogenic strains) to assess the antifungal properties of materials like paints, coatings, or textiles, preventing spoilage or degradation.
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Quality Control Strains
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Typically, specific species of Candida and Aspergillus are recommended by organizations such as CLSI and EUCAST.
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Preparation of Antifungal Agent
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Select an appropriate solvent to prepare the stock solution of the antifungal agent.
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Dilute the antibacterial agent twice continuously to prepare a series of concentration ranges.
Antifungal Agent sample types
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Types
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Details
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Established Antifungal Drugs
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Different classes of antifungals: Polyenes, Azoles, Echinocandins, Allylamines, Pyrimidine analogs, Griseofulvin.
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Various formulations
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Novel or Investigational Antifungal Compounds
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Natural products: Extracts or purified compounds from plants, microorganisms, or other natural sources.
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Synthetic compounds: Novel chemical entities designed and synthesized in laboratories.
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Biologics: Antibodies, peptides, or other biological molecules with potential antifungal activity.
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Antifungal Agents Incorporated into Materials
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Coatings: Paints, varnishes, or other surface treatments.
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Polymers: Plastics, textiles, or other polymeric materials.
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Building materials: Drywall, wood products.
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Reference standards: Known antifungal agents with established activity against the test fungi, are used to validate the test system and compare the activity of new compounds.
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Performing the Antifungal Susceptibility Test
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Broth Dilution: Add the fungal inoculum to a series of test tubes or microplate wells containing antifungal diluents.
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Agar Dilution: Add the diluted antifungal agent to the molten agar medium and then pour it into a petri dish.
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Disk Diffusion: Diffuse the standardized fungal suspension onto the agar plate, and place the paper plate impregnated with a known concentration of antifungal agent on the agar surface.
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Gradient Diffusion (Etest): Place a plastic strip containing an antifungal gradient on an AGAR plate inoculated with fungi.
Incubation
Cultivate the test substances under appropriate conditions to ensure that the fungi grow without antifungal agents.
Reading and Interpretation of Results
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After incubation, evaluate the growth of the fungi in the presence of different concentrations of antifungal agents.
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MIC: The minimum concentration of the antifungal agent that inhibits the visible growth of fungi is judged as MIC.
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Determine the minimum effective concentration (MEC): The lowest concentration at which mycelial growth is significantly hindered compared with the control.
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Result interpretation.
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Quality Control: Set up controls throughout the entire testing process to ensure the sterility of the culture medium and reagents, that is, the applicability of the operating process.
Deliverables
The definitive outputs of our antifungal activity testing furnish a thorough evaluation of a fungal isolate's responsiveness to a panel of antifungal agents. These results are delivered in a standardized, readily interpretable format, accompanied by detailed methodological descriptions and rigorous quality control metrics. This information is paramount for informing effective antifungal treatment strategies and for advancing fundamental research in the field.
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Deliverables
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Details
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Formal Report
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Sample Information
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Antifungal Agent Panel
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Testing Methodology
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Results of Antifungal Susceptibility Testing
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MIC (Minimum Inhibitory Concentration): Expressed in μg/mL or mg/L.
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Zone Diameter (if Disk Diffusion was used): Measured in millimeters.
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MEC (Minimum Effective Concentration) (if applicable): Representing significant morphological growth inhibition.
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Interpretation
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Additional services
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Antifungal Mechanism Assay
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Determination of Lesion of Plasma Membrane
Scattergram analysis was performed on each sample to assess morphological changes and the percentage of PI-positive cells was determined by flow cytometry.
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Determination of The Mitochondrial Membrane Potential (MMP)
1). Membrane potential kit.
2). The changes in MMP in fungi after antifungal treatment were analyzed by laser confocal microscopy and flow cytometry with fluorescent dyes.
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Determination of Mitochondrial ATPase Activity
The mitochondrial ATPase activity in fungal cells is measured using a Micro-ATPase Assay Kit.
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Determination of Endogenous ROS Production
Endogenous ROS levels in fungal cells are measured by a fluorometric assay using the fluorescent dye.
Turnaround Time
The typical timeframe for this service ranges from 3 to 6 weeks, depending on the complexity of the project, the number of isolates and antifungal agents, and the specific assays required. Project timelines will be clearly defined in the initial consultation and project proposal.
Case Studies
Case 1: Dose-response Assays of the Selected Molecules Against Candida albicans Biofilms (XTT Assay)
Sample: Compound was Provided by the client.
Fungus: (Provided by Creative Biolabs.)
The client provided two small molecule samples. We contracted for the dose-response assay to test the inhibitory effects on the Candida albicans biofilm formation. As shown in the figure, the molecule could inhibit biofilm formation.
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Brochures
Applications
Pharmaceutical Research and Development
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Screening Novel Antifungal Compounds
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Determining the Spectrum of Activity
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Understanding Mechanisms of Action and Resistance
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Preclinical Evaluation
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Formulation Development
Environmental and Industrial Applications
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Testing Antifungal Properties of Materials
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Assessing the Efficacy of Disinfectants and Preservatives
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Environmental Monitoring
Service Areas
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Pharmaceutical Companies
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Biotechnology Companies
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Academic and Research Institutions
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Environmental Testing Laboratories
Why Us?
Comprehensive Methodologies
We provide a wide range of AFST methods, including broth microdilution (CLSI and EUCAST), gradient diffusion, disk diffusion, automated testing, and molecular methods, ensuring we can meet diverse research needs.
Accuracy and Reliability
We adhere to strict quality control procedures and follow CLSI and EUCAST guidelines, ensuring the accuracy and reliability of our results.
Customized Solutions
We offer customized AFST panels and study designs tailored to your specific research objectives and fungal species.
Rapid Turnaround Times
Our streamlined workflows and state-of-the-art equipment enable us to deliver results on time, accelerating your research progress.
Expert Support
Our team of experienced microbiologists and infectious disease specialists provides expert consultation, data analysis, and interpretation.
State-of-the-Art Technology
Creative Biolabs utilizes advanced automated systems like VITEK 2, for high-throughput and rapid AFST.
Molecular Resistance Detection
In addition to phenotypic methods, Creative Biolabs offers molecular methods, such as PCR-based assays, to detect specific genetic mutations associated with antifungal resistance.
Quality Assurance
Creative Biolabs maintains rigorous quality control procedures, adhering to CLSI and EUCAST guidelines to ensure the accuracy and reliability of AFST results.
Ready to chat about your antifungal testing needs? Let's figure out the best approach for your project – Get a Quote Today!
FAQs
Can Creative Biolabs help with non-routine antifungal susceptibility testing?
Yes, we focus on developing and validating customized analyses to address unique research challenges.
What quality control measures are in place to ensure the accuracy of the results?
Creative Biolabs adheres to strict quality control procedures, following CLSI and EUCAST guidelines. We use calibrated equipment, validated methods, and reference strains to ensure the accuracy and reproducibility of our results. We also provide detailed quality control reports with every project. Inquire today for more details on our quality assurance process.
What types of fungal species and antifungal agents can Creative Biolabs test?
Creative Biolabs can test a wide range of fungal species, including yeasts and molds relevant to research.
Customer Reviews
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"Using Creative Biolabs' Antifungal Testing Solutions in our research has significantly improved the accuracy of our MIC determinations and streamlined our workflow." [J**n D**e]
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"Creative Biolabs' comprehensive AFST services have facilitated the identification of novel antifungal resistance mechanisms in our clinical isolates." [M**k S**h]
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"The rapid turnaround time and expert consultation provided by Creative Biolabs have accelerated our antifungal drug development program." [A**a R**z]
For Research Use Only. Not intended for use in
food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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