Comprehensive Gut Microbiota Profiling via 16S rRNA Sequencing Service

Q: Which 16S rRNA region should I target for my study?

Selecting the ideal 16S rRNA target region for your work is intrinsically linked to the precise scientific questions you aim to answer and the level of taxonomic discrimination you seek. To address this, we provide sequencing services encompassing diverse variable regions (e.g., V1-V9, V4) alongside full-length analysis. Our knowledgeable scientific staff can assist you in pinpointing the most fitting region to effectively address your research goals. Please contact us to arrange a personalized discussion.

Q: What sequencing depth is recommended for my study?

Determining the necessary depth of sequencing depends on the inherent complexity of the microbial community and the specific goal of your study. Recognizing this variability, we offer adaptable sequencing options that are designed to meet your specific study. Feel free to contact us to discuss your project needs.

Q: How do you ensure data quality and accuracy?

We uphold stringent quality control protocols throughout our entire workflow, encompassing initial sample assessment through to final bioinformatics interpretation.

Q: Can you provide customized bioinformatics analysis?

Affirmative. We provide bioinformatics analysis services specifically tailored to the unique demands of your research. Please contact us to discuss your particular analytical needs.

As scientific investigators deeply engaged in understanding the intricate dynamics of the human gut, we at Creative Biolabs offer our specialized 16S rRNA sequencing services. Leverage our rigorously validated and highly accurate methodologies to gain a thorough understanding of the gut microbiome's complex architecture, encompassing both the identity and variety of its constituent microbial communities. Our dependable, premium-quality sequencing services are purpose-built to expedite your research endeavors, enabling investigators to fully capitalize on the information-rich data generated from their microbiome studies. Request a quote

Reliable & Accurate 16S rRNA Sequencing Services

Workflow

Fig.1 16S sequencing process. (Creative Biolabs Original)

Service Details

Sample Processing

Library Preparation

Sequencing

Bioinformatics Analysis

Additional Services

Deliverables

Turnaround Time

Sample Processing and DNA Extraction

Sample reception and registration
Nucleic acid extraction: Isolation of high-quality total DNA from your gut microbiota samples (e.g., stool, biopsies). We will have optimized protocols for different sample types to maximize DNA yield and purity.
DNA quality control and quantification: This ensures that the DNA meets the requirements for downstream PCR amplification.

Sample Types

Types	Details
Stool (Feces)	This is the most common and non-invasive sample type for gut microbiota research, providing a representative overview of the microbial communities in the distal colon.
Colonic Biopsies	Collected during colonoscopies or sigmoidoscopies, these samples provide a more direct assessment of the microbiota associated with the colonic mucosa.
Rectal Swabs	Less invasive than biopsies, rectal swabs can capture some of the microbial communities present in the rectum.
Swabs from specific gut regions (in animal models)	Allowing for targeted sampling along the gastrointestinal tract.

16S rRNA Gene Amplification and Library Preparation

PCR amplification of the 16S rRNA gene
Using universal or region-specific primers targeting conserved regions flanking the hypervariable regions (V1-V9) of the 16S rRNA gene.
PCR product quality control
To confirm the expected size and absence of non-specific amplification.
Library preparation for Next-Generation Sequencing (NGS)
Library quantification
To ensure proper loading onto the sequencing instrument.

Sequencing

Next-Generation Sequencing (NGS)
Sequencing quality control
Monitoring the sequencing run and assessing the quality of the generated raw sequence data.

Bioinformatics Analysis

Taxonomic assignment
Generation of abundance tables
Alpha diversity analysis
Beta diversity analysis
Differential abundance analysis
Functional prediction (optional)

Additional Services

Custom primer design: If your research requires targeting specific microbial groups.
Full-length 16S rRNA sequencing: Providing higher taxonomic resolution compared to sequencing only variable regions.
Statistical consulting: Offering advanced statistical analysis tailored to your experimental design.
Custom bioinformatics pipelines: Adapting the analysis workflow to meet specific research needs.
Integration with other omics data: Combining 16S rRNA data with metagenomics, metatranscriptomics, or metabolomics data for a more holistic understanding.

Deliverables

Deliverable Components	Details
Reports and Visualizations	Comprehensive report: Summarizing the experimental procedures, bioinformatics pipeline, key findings, and potential interpretations of the results. Figures and graphs Metadata integration: The report and visualizations will typically incorporate any metadata you provided with your samples (e.g., treatment groups, health status) to facilitate meaningful comparisons.
Raw and Processed Sequencing Data	Raw FASTQ files: These files contain the unprocessed sequence reads generated by the sequencing instrument for each sample. They include the nucleotide sequences and their associated quality scores. This allows you to perform your downstream analysis if desired. In standard file formats (e.g., FASTQ, BAM).

Turnaround Time

The typical timeframe for this service ranges from 6 to 8 weeks, depending on the complexity of the project, sample volume, and specific analysis requirements.

Get a Quote Today → Fig.2. The colony on the plate. (Creative Biolabs Original)

Applications

Figuring out who's there in a healthy or sick gut.
Spotting microbial clues linked to conditions like obesity, IBD, or even cancer.
Seeing how diet changes or those probiotic/prebiotic things mess with your gut crew.
Understanding if your gut buddies change how medicines work.
Developing new treatments or tests that target your gut microbes.

Advantages

Pretty Precise ID: Advanced sequencing platform. It gets you a good handle on who's who down there.
Broad Strokes View: You can see the whole community without needing to know exactly what you're looking for beforehand.
Solid Foundation: It's a well-established and reliable method.
Cost-effective for Surveys: Compared to some other deep dives, it's easier on the wallet for big studies.
Lots of Data Out: There's a ton of existing research to compare your findings to.

Experience the Creative Biolabs Advantage - Get a Quote Today for a personalized consultation and let's discuss how we can help you achieve your research goals.

FAQs

Which 16S rRNA region should I target for my study?

What sequencing depth is recommended for my study?

How do you ensure data quality and accuracy?

Can you provide customized bioinformatics analysis?

Customer Reviews

"Using Creative Biolabs' 16S rRNA sequencing in our research has significantly improved the resolution of our gut microbiome analysis, allowing us to identify key microbial species involved in metabolic disorders."

J***es B

"The expert support and customized bioinformatics analysis provided by Creative Biolabs have been invaluable in helping us interpret our complex microbiome data and generate meaningful insights."

D***d K

Related Services

Creative Biolabs offers a comprehensive portfolio of microbiome research services to complement our 16S rRNA sequencing, including:

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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.