Comprehensive 16S rDNA Gene Sequencing Service for Microbe Identification
Services Applications Advantages FAQs
Creative Biolabs provides comprehensive 16S rDNA gene sequencing services, a powerful tool for bacterial identification and characterization. Our services deliver the detailed microbial information necessary for a wide range of research applications. Request a quote
Accurate Microbial Identification Services via 16S rDNA Gene Analysis
Workflow
Service Details
Sample Handling and Preparation
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Sample reception, logging, and storage under appropriate conditions upon arrival at our lab.
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Bacterial isolates or mixed community samples.
Nucleic Acid Extraction
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Extraction of high-quality microbial DNA from the submitted samples using optimized protocols based on sample type (e.g., pure culture, mixed culture, environmental samples).
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Quantification of the extracted DNA to determine concentration.
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Assessment of DNA quality (e.g., purity, integrity) to ensure suitability for PCR amplification
16S rDNA Gene Amplification
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Targeted amplification of specific variable regions (e.g., V1-V9, V3-V4) of the 16S rDNA gene using PCR with universal or bacteria-specific primers.
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Addition of unique index sequences (barcodes) to each sample's PCR product for multiplex sequencing.
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PCR Product Processing
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Quality control of the amplified fragments using gel electrophoresis to verify size and purity.
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Preparation of the purified PCR products.
Sequencing
Performing the sequencing run on the platform to generate raw sequence reads.
Bioinformatics Analysis
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Sequence Alignment: Aligning the processed reads against reference databases of 16S rDNA sequences (e.g., NCBI).
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Taxonomic Assignment: Identifying the closest matching sequences in the database to assign a taxonomic classification (e.g., genus, species) to the query sequences.
Data Reporting (Deliverables)
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Deliverable Components
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Content Specifications
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Comprehensive Report Document
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Details of the DNA extraction, PCR amplification, and sequencing procedures.
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The identified microbial species (or the closest possible identification if the species-level resolution isn't achieved).
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Quality metrics for the sequencing and analysis.
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Raw Sequencing Data
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Raw FASTQ files
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Taxonomic Assignment
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Visualizations
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Sequence Alignment Visualization: A visual representation of the alignment between your consensus sequence and the top matching sequences from the database, highlighting any differences.
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Phylogenetic Tree Snippet (sometimes included): A small phylogenetic tree showing the position of your identified microbe relative to closely related species in the database, providing context for the identification.
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Turnaround Time
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For pure microbial identification using 16S rDNA sequencing, you can generally expect a relatively quick turnaround.
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For the microbes in the mixed sample, it depends on the complexity of the sample.
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Applications
Environmental Microbiology - Identification of Isolated Environmental Bacteria
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Characterize the bacteria isolated from different environments.
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Understanding microbial diversity and ecological roles.
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Identifying potential bioremediation agents.
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Discovering novel microorganisms with unique properties.
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Monitoring environmental changes and impacts on microbial communities.
Food Microbiology - Identification of Bacteria in Food Products
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Identifying spoilage organisms, probiotics, or potential pathogens in food samples.
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Identifying contaminants in food production.
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Characterizing beneficial bacteria in fermented foods.
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Investigating the causes of foodborne illnesses.
Industrial Microbiology and Biotechnology - Identification of Microbes in Industrial Processes
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Identifying bacteria involved in fermentation, bioproduction, or biodegradation processes.
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Quality control of industrial cultures.
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Troubleshooting contamination issues.
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Characterizing microorganisms with potential for biotechnological applications.
Pharmaceutical Microbiology
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Identification and validation of microorganisms used in the production of biological drugs.
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Identify bacterial contamination in drug preparations.
Research and Taxonomy
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Identify and classify newly isolated bacteria.
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Verify the identity and purity of the strains in the culture set.
Advantages
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Precise Identification: Leveraging advanced sequencing technologies and a rigorous bioinformatics pipeline, we ensure the accurate and dependable identification of bacterial species.
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Fast Turnaround Time: Optimized protocol, simplified workflow.
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Expert Support: Experienced scientists and bioinformaticians.
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Customizable Solutions: Highly adaptable and fully customized solutions.
Contact us today
Creative Biolabs offers 16S rDNA gene sequencing services to deliver precise, streamlined, and thorough microbial identification results. Our expert team, combined with cutting-edge technology platforms and an accurate understanding of our client's research goals, makes us a reliable partner for researchers. Contact us today to discuss your project requirements.
FAQs
How accurate is 16S rDNA sequencing for bacterial identification?
16S rDNA sequencing usually provides a high level of confidence in bacterial identification, but the reliability of the results is also related to factors such as the integrity of the sample, the sequencing platform, and subsequent bioinformatics analysis. At Creative Biolabs, we implement strict quality assurance agreements to ensure the reliability of data to the greatest extent.
Which types of samples can be tested by 16S rDNA sequencing?
The sample types for 16S rDNA sequencing are relatively extensive, including pure bacterial cultures, mixed microbial communities, and complex environmental samples. Creative Biolabs has experience in handling various types of samples. Contact our team to determine the best approach for your samples.
How long will it take to get the result?
The turnaround time of 16S rDNA sequencing is usually between 2 and 4 weeks. Creative Biolabs will provide a specific project schedule and update the project progress on time.
What kind of data analysis is included?
Creative Biolabs offers comprehensive bioinformatics analysis, including quality filtering, sequence assembly, classification, and statistical analysis. We will provide a detailed report with clear and concise results.
Customer Reviews
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"Using Creative Biolabs' 16S rDNA sequencing in our environmental research has significantly improved our ability to characterize microbial communities and understand their role in ecosystem function." [Joh***on]
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"Creative Biolabs' 16S rDNA sequencing service provided highly accurate and reliable bacterial identification, which was crucial for our infectious disease study. The fast turnaround time was also a major advantage." [Sar***h]
For Research Use Only. Not intended for use in
food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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