Enterococcus faecium is a Gram-positive bacterium. It can be commensal in the gastrointestinal tract of humans and animals.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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LBST-098FG |
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Product Information | |
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Product Overview | Enterococcus faecium is a Gram-positive bacterium. It can be commensal in the gastrointestinal tract of humans and animals. |
Target | Enterococcus |
Genus | Enterococcus |
Strain Designation | 1.551 |
Application | Study and research |
Type Strain | No |
Culture Medium | BHI |
Culture Conditions | 28°C; Aerobiotic |
Characteristics | The thalli were spherical, arranged in pairs or piles, and gram-positive. |
Risk Group | 1 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
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Introduction | Enterococcus are lactic acid bacteria; LAB comprising both pathogenic and commensal microorganisms ubiquitous in environment even as gut symbionts. They are extensively studied as potential candidate probiotics. Several number of Enterococcus strains have been reported to produce antimicrobial compounds including bacteriocins. Bacteriocin production have be applied to preservation of a wide range of food products and is now being considered as a probiotic trait. Besides, bacteriocins are considered as promising alternative to fight emerging antimicrobial resistance. Enterococcus probiotics can be used in treatment and/or prevention of certain human and animal diseases such as alleviation of irritable bowel syndrome symptoms and antibiotic-induced diarrhea and prevention of different functional and chronic intestinal diseases. Moreover, some Enterococcus exhibit anticarcinogenic, hypocholesterolemic, as well as immune regulation effects. |
Alternative Names | Enterococcus faecium; 1.551 |
In in vitro experiments, Enterococcus faecium; 1.551 can be added directly to cell cultures at a concentration determined by preliminary dose-response studies. It is important to consider the MOI (Multiplicity of Infection) and the specific requirements of the cell lines used.
For long-term storage, Enterococcus faecium; 1.551 should be stored in cryoprotectant such as 15% glycerol at -80°C. This method helps preserve the viability and genetic integrity of the bacteria for extended periods.
Enterococcus faecium; 1.551 can be quantified using plate count methods, quantitative PCR, or flow cytometry. These techniques allow accurate measurement of bacterial concentration in different samples, facilitating research on its growth and effects.
The growth curve can be monitored by measuring optical density at 600 nm (OD600) at regular intervals. This helps in understanding the lag, log, stationary, and death phases of bacterial growth under specific experimental conditions.
DNA extraction can be performed using commercial kits designed for Gram-positive bacteria, involving enzymatic lysis, protein precipitation, and DNA purification steps. Ensuring complete cell lysis is crucial for high-quality DNA.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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