Bacteroides acidifaciens A40 is an anaerobe, mesophilic bacterium that was isolated from caecal content; wildtype C57BL/6 mouse.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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LBSX-0522-GF18
Product Information
Product Overview
Bacteroides acidifaciens A40 is an anaerobe, mesophilic bacterium that was isolated from caecal content; wildtype C57BL/6 mouse.
Target
Bacteroides
Genus
Bacteroides
Strain Designation
A40
Application
Study and research
Type Strain
Yes
Culture Medium
Columbia blood agar
Culture Conditions
37°C; Anaerobic
Source
Caecal content; wildtype C57BL/6 mouse
Risk Group
1
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Bacteroides is a group of promising candidates that has attracted major attentions from scientists as model organisms or community for the investigations of the intestinal microbiota field, due to their powerful adaption characteristics in the host and especially the underlying benefits. Bacteroides is a genus of Gram-negative, obligate anaerobic bacteria. Bacteroides species are non endospore-forming bacilli, and may be either motile or nonmotile, depending on the species. Bacteroides; order is the most abundant group of Gram-negative bacteria which flourish in the human intestine at high densities of 109-1011 CFU per gram of feces, and constitutes over 30 species which are more closely related than members in other bacteria orders. And, Bacteroides is considered as one of the major genera of the core microbiota module, of which species with relative abundance of over 5% refers to B. uniformis, B. vulgatus, B. caccae, and B. thetaiotaomicron. In addition to the unique physiological characteristics and the special ecological dynamics, Bacteroides species are under investigations for their underlying beneficial dialogues with the host. Bacteroides species to be considered as next-generation probiotics, and the application of which in prevention or intervention in dysbiosis of gut microbiota and the associated disorders requires further investigations in the modulatory mechanisms and safety evaluation. Bacteroides is one of the dominant contributors of SCFA among the intestinal commensals, mostly in the form of acetate and propionate. Accumulated acetate blocks the transportation of toxins between gut lumen and blood, while propionate is capable of inducing the apoptosis of human colon carcinoma cells, and thus avoids the formation of tumors. Moreover, Vitamin K, which is mainly synthesized by gut microbiota candidates such as Bacteroides, can help prevent or treat osteoporosis by increasing bone mineral density.
Alternative Names
Bacteroides acidifaciens Miyamoto and Itoh 2000
FAQsPublished Data Customer Reviews Related Products
How should Bacteroides acidifaciens be revived from a frozen state?
To revive Bacteroides acidifaciens from a frozen state, quickly thaw the frozen culture at room temperature and inoculate it into an appropriate anaerobic growth medium pre-warmed to 37°C. Ensure that the culture is maintained under strict anaerobic conditions to optimize recovery and growth.
What type of medium is recommended for culturing Bacteroides acidifaciens?
Bacteroides acidifaciens should be cultured in an anaerobic medium that supports the growth of anaerobic gut bacteria. This medium should contain complex carbohydrates and be supplemented with nutrients like yeast extract, which supports the bacterium's growth requirements.
Can Bacteroides acidifaciens be used in research related to dietary interventions?
Yes, Bacteroides acidifaciens is a significant subject in studies exploring the impact of diet on gut microbiota. Its abundance has been linked to the consumption of high-fiber diets and it is of interest in research for its potential to modify gut microbiota profiles during dietary interventions.
Can Bacteroides acidifaciens be genetically modified for experimental purposes?
Genetic modification of Bacteroides acidifaciens is possible and can be useful for studies on gene function and metabolic pathways. Techniques such as electroporation can be used to introduce plasmid DNA into the bacterium under anaerobic conditions.
How can the growth and metabolic activity of Bacteroides acidifaciens be assessed in the laboratory?
The growth of Bacteroides acidifaciens can be monitored by measuring optical density or viable cell counts on suitable anaerobic agar plates. Metabolic activity can be assessed by analyzing the production of short-chain fatty acids or other metabolites using gas chromatography or high-performance liquid chromatography (HPLC).
Phylogenetic tree based on 16S rRNA sequences of murine isolates and type strains of Bacteroides and Parabacteroides
The research focuses on Bacteroides acidifaciens and its interaction within the mouse intestinal microbiome, particularly its role in maintaining a balanced microbial community and influencing host physiology. The study utilizes species-specific oligonucleotide probes for fluorescence in situ hybridization (FISH) to identify and analyze the composition of Bacteroides and Parabacteroides within the caecum of conventional and specific pathogen-free (SPF) mice. Notably, Bacteroides acidifaciens was found to be dominant in both conventional and SPF mice from two different breeders, underscoring its prevalent and significant presence. Experimentally, the specificity of these probes was validated against various Bacteroides species, ensuring accurate detection. The findings reveal that the microbiota composition varied significantly among mice from different breeders, highlighting the influence of microbial diversity on host health. The study demonstrates the importance of Bacteroides acidifaciens in stabilizing gut microbiota and potentially affecting the host's immune responses, suggesting implications for understanding disease mechanisms and developing microbial-based therapies.
Momose, Y., Park, S. H., et al. Design of species-specific oligonucleotide probes for the detection of Bacteroides and Parabacteroides by fluorescence in situ hybridization and their application to the analysis of mouse caecal Bacteroides-Parabacteroides microbiota. Journal of applied microbiology. 2011, 111(1): 176-184.
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