Search

Precisely Quantify Tumor Endophytic Bacteria: Absolute Quantitative Sequencing Service

Creative Biolabs specializes in providing the most advanced absolute quantitative sequencing services for tumor endophytic bacteria. Unlike traditional relative abundance sequencing approaches, our service offers precise enumeration of bacterial taxa within tumor samples, providing invaluable insights into the actual microbial load and its potential impact. We leverage advanced molecular biology techniques combined with robust bioinformatics analysis to deliver accurate, reliable, and actionable data to researchers. Request a quote

Our Comprehensive Tumor Endophytic Bacteria Absolute Quantitative Sequencing Services

Workflow

Fig.1 Workflow of metagenomic absolute quantitative analysis. (Creative Biolabs original)

Service Details

Samples
Turnaround Time
Deliverables

Sample Information & Requirements

Sample Types
  • Fresh Frozen Tumor Tissue
  • FFPE Tumor Tissue (Formalin-Fixed Paraffin-Embedded) - Please note: FFPE samples may have lower DNA quality and require specialized extraction protocols, potentially impacting sensitivity.
  • Tumor Biopsies
  • Tumor Cell Lines
  • Tumor-Adjacent Normal Tissue (for comparative analysis)
  • Biofluids (e.g., plasma, serum, ascites fluid, cerebrospinal fluid) if tumor-derived bacteria are suspected.
Sample Requirements
  • DNA
    • ≥50μL
  • Fresh Frozen Tissue
    • Minimum 200 mg of tissue.
    • Flash-frozen immediately after collection and stored at -80°C.
    • No visible necrotic areas or excessive non-tumor tissue, if possible.
  • FFPE Tissue
    • Minimum 5-10 sections (10-20 µm thickness) or ≥5 blocks.
    • Avoid excessive fixation time, as this can degrade DNA.
  • Tumor Biopsies
    • Minimum 2-3 core biopsies.
    • Flash-frozen or placed in an appropriate stabilization buffer (e.g., RNAlater) immediately.
  • Tumor Cell Lines
    • Minimum 1×106 cells.
    • Pelleted and flash-frozen.
  • Biofluids
    • Minimum 500 µL for plasma/serum, larger volumes for other fluids.
    • Collected using sterile techniques and stored at -80°C.
General Requirements for All Sample Types
  • Sterile Collection: All samples must be collected under strict aseptic conditions to minimize external microbial contamination.
  • Appropriate Storage & Shipping: Samples should be shipped on dry ice or with cold packs, depending on the sample type and transit time, in clearly labeled, robust containers.
  • Detailed Sample Information: Provide comprehensive metadata for each sample, including tumor type, stage, patient demographics, treatment history, and any relevant clinical data. This information is crucial for meaningful data interpretation.

Turnaround Time

Typical turnaround time for our Absolute Quantitative Sequencing Service is 4-8 weeks from the receipt of qualified samples to the delivery of the final report. Project timelines may vary depending on the complexity of the study, the number of samples, and specific analyses requested. Expedited services may be available upon request.

Delivery Standards

Deliverable Components Content Specifications
Raw Sequencing Data
  • FASTQ files of your demultiplexed reads.
Bioinformatics Report
  • Summary of quality control metrics.
  • Taxonomic composition at various phylogenetic levels (phylum to species).
  • Absolute abundance quantification for each identified taxon (e.g., copies per gram/mL).
  • Alpha and beta diversity analyses.
  • Relevant statistical comparisons between sample groups (if applicable).
  • Visualization of results (e.g., bar plots, heatmaps, PCoA plots).
Customized Data Tables
  • Spreadsheet files containing absolute and relative abundance data.

Get a quote today

Advantages of Our Service

  • True Absolute Quantification: Going beyond the concept of relative quantity to gain a deeper understanding of the actual quantity of each bacterial classification group.
  • High Sensitivity & Specificity: Optimized protocols and cutting-edge sequencing technologies ensure accurate detection and identification of even low-abundance bacteria.
  • Comprehensive Analysis: From DNA extraction to advanced bioinformatics and biological interpretation, we provide an end-to-end solution.
  • Expert Team: Our scientists have extensive experience in microbiome research, oncology, and bioinformatics.
  • Customizable Solutions: We work closely with clients to tailor services to specific research questions and project needs.
  • Rigorous Quality Control: Strict QC measures at every stage of the workflow guarantee reliable and reproducible data.
  • Actionable Insights: Our reports provide clear, interpretable results with biological context, facilitating informed decision-making.
Fig.2 Metagenomic absolute quantitative analysis. (Creative Biolabs Authorized)

Applications of Metagenomic Absolute Quantitative Sequencing

Cancer Research

  • Investigating the specific roles of individual bacterial taxa in tumor initiation, progression, metastasis, and drug resistance.
  • Search for new microbial biomarkers that can be used for early cancer detection, prognosis assessment, and prediction of treatment responses.
  • Exploring the interplay between tumor microbiome, host immunity, and therapeutic efficacy (e.g., immunotherapy, chemotherapy).
  • Understanding the influence of the tumor microbiome on the tumor microenvironment.

Drug Discovery & Development

  • Preclinical and clinical studies assessing the impact of new anti-cancer drugs on the tumor microbiome.
  • Identifying bacterial species that metabolize drugs or contribute to drug resistance.
  • Developing microbiome-modulating therapies for cancer.

Translational Medicine

  • Translating research findings into clinically actionable insights.
  • Developing diagnostic assays based on absolute bacterial quantification.
  • Monitoring treatment response and recurrence based on changes in the tumor endophytic bacterial profile.

By providing precise, absolute quantification of tumor endophytic bacteria, we empower our clients to uncover critical insights into cancer biology and pave the way for innovative diagnostic and therapeutic strategies. Partner with us to unlock the full potential of tumor microbiome research.

FAQs

Why is absolute quantification important for tumor endophytic bacteria?

The total bacterial load within a tumor can be highly variable. Absolute quantification provides a more accurate picture of the microbial burden and allows for direct comparison of specific bacterial populations across different tumor samples or conditions, which is critical for understanding their biological impact.

Can you handle FFPE samples?

Yes, we can process FFPE samples, but it's important to note that DNA degradation can occur with FFPE preservation. We employ specialized extraction methods to maximize DNA yield and quality from these samples, but sensitivity may be lower compared to fresh frozen tissue.

What kind of reporting do I receive?

You will receive a comprehensive report including raw data, processed data tables, detailed bioinformatics analysis, graphical visualizations, and a summary of key findings with scientific interpretation.

Online Inquiry

For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.

Live Biotherapeutic


ISO 9001 Certified - Creative Biolabs Quality Management System.
Contact us
Solutions

Copyright © 2025 Creative Biolabs. All Rights Reserved.

Inquiry Basket