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Precise Quantification: Absolute Quantitative ITS Fungal Microbiome Sequencing Service

At Creative Biolabs, we are at the forefront of microbiome research, offering a cutting-edge Absolute Quantitative ITS Fungal Microbiome Sequencing Service. This specialized service provides a precise and comprehensive characterization of fungal communities within diverse biological and environmental samples. Unlike traditional relative abundance methods, our absolute quantification approach delivers not only the taxonomic composition but also the actual number of fungal cells or genomic copies present, offering unparalleled insights into fungal load and dynamics. This service is invaluable for researchers seeking to understand the role of fungi in health, disease, and various ecosystems with quantitative accuracy. Request a quote

Our Comprehensive Absolute Quantitative ITS Fungal Sequencing Services

Workflow

Fig.1 Workflow of fungal absolute quantitative analysis. (Creative Biolabs original)

Service Details

Samples
Turnaround Time
Deliverables

Sample Information & Requirements

Sample Types: We accept a wide range of sample types, including, but not limited to:

Sample Types
  • Environmental: Soil, water, sediment, air filters, plant tissues.
  • Research: Fecal samples, skin swabs, oral swabs, tissue biopsies.
  • Agricultural: Plant roots, rhizosphere soil, compost.
  • Food & Beverage: Fermentation samples, spoilage analysis.
Sample Requirements (DNA)
  • Concentration: Minimum 20 ng/µL.
  • Volume: Minimum 25 µL.
  • Purity: A260/280 ratio between 1.8-2.0; A260/230 ratio > 2.0.
  • Integrity: High molecular weight DNA (visualized on agarose gel as a clear band).
  • Buffer: Eluted in sterile nuclease-free water or low-EDTA TE buffer.
Sample Requirements (Raw Biological Samples)
  • Please contact us for specific guidance on your sample type. Proper collection and storage (e.g., freezing at -80°C or immediate DNA extraction) are crucial for preserving microbial integrity.

Turnaround Time

Our standard turnaround time for the Absolute Quantitative ITS Fungal Microbiome Sequencing Service is typically 6-8 weeks from the receipt of qualified samples. This duration includes DNA extraction, library preparation, sequencing, and comprehensive bioinformatics analysis. Expedited services may be available upon request; please inquire during your initial consultation.

Delivery Standards

Deliverable Components Content Specifications
Raw Sequencing Data
  • FASTQ files of your demultiplexed reads.
Processed Data Files
  • ASV/OTU tables with absolute abundance counts.
  • Taxonomic assignments (from Kingdom to Species level, where possible).
  • Phylogenetic trees.
Comprehensive Bioinformatics Report
  • Executive summary of key findings.
  • Detailed methodology.
  • The charts and tables presenting the composition of the fungal community, alpha diversity, beta diversity, and statistical comparisons
  • Interpretation of results and their biological significance.

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Our Service Advantages

  • Unrivaled Accuracy: Provides true absolute quantification of fungal taxa, overcoming the limitations of relative abundance data. This is crucial for comparing fungal loads across samples, especially when total biomass varies.
  • High Resolution: Utilizes the ITS region for precise species-level identification, enabling granular insights into fungal communities.
  • Comprehensive Data: Delivers both qualitative (what's there) and quantitative (how much is there) information.
  • Robust & Reproducible: Our validated protocols and rigorous quality control ensure reliable and reproducible results.
  • Expert Support: Benefit from our team's extensive expertise in fungal microbiology, sequencing, and bioinformatics.
  • Customizable Solutions: Tailored experimental designs and bioinformatics analyses to meet your specific research needs.
  • Improved Biological Interpretation: Enables more accurate interpretation of microbial dynamics in response to interventions, disease states, or environmental changes.
Fig.1 Development of diagnostic tools for fungal infections. (Creative Biolabs Authorized)

Target Customer Groups

  • Academic Researchers: Seeking precise and reproducible fungal microbiome data for publications and grant applications in ecology, environmental science, plant pathology, human health, and veterinary medicine.
  • Pharmaceutical and Biotechnology Companies: Investigating the role of fungi in drug development, disease pathogenesis, and therapeutic interventions.
  • Agricultural and Food Industries: Studying fungal communities in soil health, plant diseases, crop protection, food spoilage, and fermentation processes.
  • Environmental Monitoring Agencies: Assessing fungal contamination, biodiversity, and ecosystem health.
  • Cosmetic and Personal Care Companies: Exploring fungal interactions on skin and other surfaces.
  • CROs and Contract Labs: Looking to outsource specialized fungal microbiome analysis with high precision.

Choose our Absolute Quantitative ITS Fungal Microbiome Sequencing Service for unparalleled accuracy and depth in your fungal microbiome research. Contact us today to discuss your project and receive a customized quote.

FAQs

Why is absolute quantification important for fungal microbiome studies?

Relative abundance data can be misleading when comparing samples with varying total fungal biomass. An increase in the relative abundance of one fungal species might simply be due to a decrease in another, rather than an actual increase in its absolute numbers. Absolute quantification provides the true cellular or genomic load of each fungal taxon, allowing for direct comparisons of fungal burden and more accurate biological conclusions.

What is the difference between ITS1 and ITS2 sequencing, and which do you use?

The ITS region is usually divided into ITS1 (located between the 18S and 5.8S ribosomal RNA genes) and ITS2 (located between the 5.8S and 28S ribosomal RNA genes). Both of these regions exhibit high variability and are very useful for the identification of fungi.

What kind of bioinformatics analysis is included?

Our standard analysis includes raw data quality control, ASV/OTU clustering, taxonomic assignment, absolute abundance calculation, alpha and beta diversity analysis, and relevant statistical comparisons. We can also provide customized analyses based on your project's needs.

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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.

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