Enterococcus lactis is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from feces of tumor patients.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
Product Information | |
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Product Overview | Enterococcus lactis is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from feces of tumor patients. |
Target | Enterococcus |
Genus | Enterococcus |
Strain Designation | ZCYMa05 |
Application | Study and research |
Type Strain | No |
Culture Medium | MRS |
Culture Conditions | 37°C; Anaerobic |
Source | Feces of tumor patients |
Risk Group | 1 |
Product Format | Freeze-dried |
Packaging | Ampoule tube |
Storage | -80°C |
Shelf Life | 6 years |
Target Introduction | |
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Introduction | Enterococcus are lactic acid bacteria; LAB comprising both pathogenic and commensal microorganisms ubiquitous in environment even as gut symbionts. They are extensively studied as potential candidate probiotics. Several number of Enterococcus strains have been reported to produce antimicrobial compounds including bacteriocins. Bacteriocin production have be applied to preservation of a wide range of food products and is now being considered as a probiotic trait. Besides, bacteriocins are considered as promising alternative to fight emerging antimicrobial resistance. Enterococcus probiotics can be used in treatment and/or prevention of certain human and animal diseases such as alleviation of irritable bowel syndrome symptoms and antibiotic-induced diarrhea and prevention of different functional and chronic intestinal diseases. Moreover, some Enterococcus exhibit anticarcinogenic, hypocholesterolemic, as well as immune regulation effects. |
Alternative Names | Enterococcus lactis |
Enterococcus lactis; ZCYMa05 should be cultured in MRS broth or agar, which provides the necessary nutrients for optimal growth. This medium supports the proliferation of lactic acid bacteria under anaerobic conditions at 37°C.
For long-term storage, Enterococcus lactis; ZCYMa05 should be kept at -80°C in a cryoprotectant such as glycerol. This method preserves the bacterial viability and genetic stability, ensuring the strain remains suitable for future research applications.
Verification of Enterococcus lactis; ZCYMa05 identity can be performed using molecular techniques such as 16S rRNA sequencing, PCR, and MALDI-TOF mass spectrometry. These methods confirm the strain's genetic and phenotypic characteristics.
Quantification of Enterococcus lactis; ZCYMa05 in a sample can be achieved using plate count methods on selective media, flow cytometry, or quantitative PCR (qPCR). These techniques provide accurate measurements of bacterial concentration.
For in vivo experiments, Enterococcus lactis; ZCYMa05 should be cultured to the desired density, harvested, and then resuspended in a suitable buffer or medium. Ensuring the correct concentration and viability is crucial for consistent and reliable results in animal studies.
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For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
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