Bacteroides fragilis was isolated from appendix abscess. It is an anaerobic, Gram-negative, pleomorphic to rod-shaped bacterium.
For Research Use Only. Not intended for use in food manufacturing or medical procedures (diagnostics or therapeutics). Do Not Use in Humans.
CAT
Size
Price
Quantity
LBST-013FG
Product Information
Product Overview
Bacteroides fragilis was isolated from appendix abscess. It is an anaerobic, Gram-negative, pleomorphic to rod-shaped bacterium.
Target
Bacteroides
Genus
Bacteroides
Strain Designation
VPI 2553
Application
Media testing; Quality control strain; Susceptibility testing; Quality control strain for BBL and MicroScan.
Culture Medium
Columbia blood agar
Culture Conditions
37°C; Anaerobic
Source
Appendix abscess
Risk Group
2
Product Format
Freeze-dried
Packaging
Ampoule tube
Storage
-80°C
Shelf Life
6 years
Target Introduction
Introduction
Bacteroides is a group of promising candidates that has attracted major attentions from scientists as model organisms or community for the investigations of the intestinal microbiota field, due to their powerful adaption characteristics in the host and especially the underlying benefits. Bacteroides is a genus of Gram-negative, obligate anaerobic bacteria. Bacteroides species are non endospore-forming bacilli, and may be either motile or nonmotile, depending on the species. Bacteroides; order is the most abundant group of Gram-negative bacteria which flourish in the human intestine at high densities of 109-1011 CFU per gram of feces, and constitutes over 30 species which are more closely related than members in other bacteria orders. And, Bacteroides is considered as one of the major genera of the core microbiota module, of which species with relative abundance of over 1% refers to B. uniformis, B. vulgatus, B. caccae, and B. thetaiotaomicron. In addition to the unique physiological characteristics and the special ecological dynamics, Bacteroides species are under investigations for their underlying beneficial dialogues with the host. Bacteroides species to be considered as next-generation probiotics, and the application of which in prevention or intervention in dysbiosis of gut microbiota and the associated disorders requires further investigations in the modulatory mechanisms and safety evaluation. Bacteroides is one of the dominant contributors of SCFA among the intestinal commensals, mostly in the form of acetate and propionate. Accumulated acetate blocks the transportation of toxins between gut lumen and blood, while propionate is capable of inducing the apoptosis of human colon carcinoma cells, and thus avoids the formation of tumors. Moreover, Vitamin K, which is mainly synthesized by gut microbiota candidates such as Bacteroides, can help prevent or treat osteoporosis by increasing bone mineral density.
Alternative Names
Bacteroides fragilis
FAQs Customer Reviews Related Products
What are the specific handling procedures for Bacteroides fragilis?
Bacteroides fragilis; requires strict anaerobic handling procedures to maintain viability. Utilize anaerobic chambers or jars with gas packs, and ensure minimal exposure to oxygen during transfers and culturing.
What media is recommended for culturing Bacteroides fragilis?
Bacteroides fragilis; thrives in media such as BHI (Brain Heart Infusion) broth supplemented with hemin and vitamin K. This supports its growth requirements and ensures optimal cell density for experiments.
How can Bacteroides fragilis be revived from frozen stocks?
To revive Bacteroides fragilis; , quickly thaw the frozen vial at 37°C, then immediately inoculate into pre-reduced anaerobic media. Incubate under anaerobic conditions to achieve maximum recovery and viability.
What techniques are best for assessing the purity of Bacteroides fragilis cultures?
Purity of Bacteroides fragilis; can be assessed by streaking onto selective agar plates and checking for uniform colony morphology. PCR-based methods targeting specific genetic markers can also verify strain purity.
What are the considerations for long-term storage of Bacteroides fragilis?
For long-term storage, Bacteroides fragilis; should be cryopreserved in glycerol stocks at -80°C. It is crucial to aliquot stocks into small volumes to prevent multiple freeze-thaw cycles, which can reduce viability.
What are the specific handling procedures for Bacteroides fragilis; VPI 2553?
Bacteroides fragilis; VPI 2553 requires strict anaerobic handling procedures to maintain viability. Utilize anaerobic chambers or jars with gas packs, and ensure minimal exposure to oxygen during transfers and culturing.
What media is recommended for culturing Bacteroides fragilis; VPI 2553?
Bacteroides fragilis; VPI 2553 thrives in media such as BHI (Brain Heart Infusion) broth supplemented with hemin and vitamin K. This supports its growth requirements and ensures optimal cell density for experiments.
How can Bacteroides fragilis; VPI 2553 be revived from frozen stocks?
To revive Bacteroides fragilis; VPI 2553, quickly thaw the frozen vial at 37°C, then immediately inoculate into pre-reduced anaerobic media. Incubate under anaerobic conditions to achieve maximum recovery and viability.
What techniques are best for assessing the purity of Bacteroides fragilis; VPI 2553 cultures?
Purity of Bacteroides fragilis; VPI 2553 can be assessed by streaking onto selective agar plates and checking for uniform colony morphology. PCR-based methods targeting specific genetic markers can also verify strain purity.
What are the considerations for long-term storage of Bacteroides fragilis; VPI 2553?
For long-term storage, Bacteroides fragilis; VPI 2553 should be cryopreserved in glycerol stocks at -80°C. It is crucial to aliquot stocks into small volumes to prevent multiple freeze-thaw cycles, which can reduce viability.
Superior Quality for Advanced Research
Creative Biolabs' Bacteroides fragilis; has proven essential for our advanced immunology studies, providing consistent and reliable results, setting it apart from competitors.
Unparalleled Performance
The Bacteroides fragilis strain from Creative Biolabs offers unparalleled genetic stability, which crucially enhances the reproducibility of our gastrointestinal research.
Leading Choice for Microbial Studies
For cutting-edge microbial interaction studies, Creative Biolabs' Bacteroides fragilis; is the leading choice, thanks to its robust growth and genetic fidelity.
Trusted for Consistency
The Bacteroides fragilis; strain from Creative Biolabs delivers unmatched consistency and viability, making it a trusted resource in our pharmacological research.
Exceptional Purity
The purity of Creative Biolabs' Bacteroides fragilis is outstanding. It significantly enhances the accuracy of our inflammation studies, providing clear and reproducible results every time.
Reliable Storage
Creative Biolabs' Bacteroides fragilis ensures excellent long-term viability when cryopreserved. This reliability has streamlined our workflow in microbiota and immune response research.
Optimal Growth Bacteroides fragilis exhibits robust growth under anaerobic conditions, making it ideal for detailed gut microbiota analyses. This product has significantly contributed to our research advancements.
Consistent Performance
The consistent performance of Bacteroides fragilis from Creative Biolabs in our lab is commendable. It has proven invaluable for our studies on microbial interactions and host-pathogen dynamics.
High-Quality Results
The Bacteroides fragilis product has delivered high-quality results in our immune modulation studies. Its stability and purity are essential for obtaining reliable data.
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