A. soehngenii and Its Unique Functional Potential
Anaerobutyricum soehngenii, a prominent gut microbiome species, is gaining increasing recognition due to its specialized role in metabolic regulation and gut health. Classified taxonomically within the phylum Firmicutes, class Clostridia, and family Lachnospiraceae, this strictly anaerobic and non-spore-forming bacterium is an influential component of the intestinal microbiota. Unlike many other probiotics, its growth demands strict anaerobic conditions, making its cultivation uniquely challenging but also highly rewarding for advanced microbiome research.
This species is especially distinguished by its robust butyrate production capability. Butyrate, a crucial short-chain fatty acid (SCFA), significantly influences gut integrity, barrier function, and metabolic homeostasis. Additionally, A. soehngenii plays a pivotal role in bile acid metabolism, aiding the modulation of bile acid pools, which further enhances its therapeutic potential in metabolic health research.
Why A. soehngenii Matters
Emerging scientific evidence underscores the therapeutic importance of A. soehngenii, particularly regarding metabolic health. The organism's capability for substantial butyrate production underpins its ability to enhance insulin sensitivity. This was elegantly demonstrated by Udayappan et al.1, who observed significant improvements in insulin signaling pathways linked to increased colonization by A. soehngenii, indicating its direct relevance in addressing metabolic dysregulation.
Further, the bacterium's involvement in intestinal homeostasis underscores its role in preventing dysbiosis—a crucial factor in many gut-related disorders. Its presence is consistently correlated with improved intestinal barrier integrity and decreased inflammatory responses, thereby enhancing overall gut health. Notably, A. soehngenii participates actively in metabolic cross-feeding, promoting microbial ecosystem stability and resilience. Despite these promising functional attributes, the anaerobic nature of this microbe makes it notoriously challenging to cultivate and stabilize, necessitating specialized approaches and methodologies for isolation and characterization, which Creative Biolabs specializes in.
A. soehngenii vs. Other Next-Gen Probiotics
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Feature
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A. soehngenii
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A. muciniphila
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F. prausnitzii
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Butyrate Production
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High
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Low
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High
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Mucin Degradation
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No
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Yes
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No
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Glucose Homeostasis Support
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Yes
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Yes
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Yes
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Oxygen Sensitivity
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Extreme anaerobe
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Moderate
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Extreme anaerobe
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Culturing Difficulty
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High
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Medium
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High
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Clearly, A. soehngenii offers unique metabolic and functional characteristics that stand apart even among leading next-generation probiotics.
Workflow and Technologies in A. soehngenii Development
A. soehngenii Development Workflow
A. soehngenii Development Technologies
Creative Biolabs employs cutting-edge technologies to support A. soehngenii probiotic research, including:
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Flow cytometry: Precision viability assessments and bacterial enumeration.
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GC-MS: Quantitative analysis of short-chain fatty acids, specifically butyrate, acetate, and propionate.
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16S rRNA and Whole-genome sequencing (WGS): Ensuring taxonomic accuracy and detailed genomic insights.
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In vitro assays: Evaluating mucin-adherence capability, tight junction integrity, and anti-inflammatory activity through standardized cell-based methodologies.
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Custom A. soehngenii Development Services
Creative Biolabs provides tailored A. soehngenii solutions through comprehensive service modules designed to support end-to-end probiotic development:
We expertly establishes strict anaerobic culture conditions to selectively isolate A. soehngenii from fecal or intestinal samples, ensuring optimal recovery, viability, and strain purity essential for rigorous downstream probiotic characterization and research applications.
Utilizing advanced 16S rRNA and WGS methodologies, Creative Biolabs accurately identifies and characterizes A. soehngenii strains, enabling detailed taxonomic confirmation, genomic insights, and reliable differentiation necessary for comprehensive microbial analyses.
Through sophisticated analytical technologies, such as GC-MS and HPLC, Creative Biolabs precisely quantifies SCFAs including butyrate, acetate, and propionate, delivering essential metabolic profile information for validating probiotic functionality and efficacy in research models.
We conduct meticulous minimum inhibitory concentration (MIC) profiling and resistance gene screening of A. soehngenii under strictly controlled anaerobic environments, ensuring precise data for evaluating antibiotic resistance profiles critical for safe probiotic research and application.
We evaluate the immune-modulating properties of A. soehngenii through comprehensive cytokine release assays, macrophage co-culture studies, and epithelial barrier integrity tests, providing critical mechanistic insights necessary for developing scientifically validated probiotic research applications.
We offer specialized lab-scale anaerobic fermentation systems designed specifically to cultivate A. soehngenii efficiently, maintaining stringent environmental parameters essential for consistent strain performance, reproducibility, and scalability in probiotic research and preclinical development.
Employing advanced purification methodologies, we reliably eliminate contaminants and enriches A. soehngenii strains, achieving high-level purity required for dependable and precise downstream experimentation, characterization studies, and robust probiotic product development efforts.
Offering extensive preliminary biosafety assessments, we carefully evaluate A. soehngenii strains via hemolysis, cytotoxicity, and endotoxin detection assays, delivering vital safety data supporting regulatory requirements and fostering confidence in further probiotic-related research development activities.
Collaborating with Creative Biolabs offers clients unmatched expertise in handling strictly anaerobic bacteria such as A. soehngenii. With a robust platform integrating high-throughput screening capabilities, precise metabolic profiling, and rigorous immunological assessments, Creative Biolabs supports comprehensive, scientifically robust probiotic development. The extensive experience and technology-driven approach ensure a seamless progression from initial strain isolation through rigorous preclinical validation, meeting stringent research standards.
A. soehngenii-Related Products
Strain Repository
Creative Biolabs offers a diverse collection of A. soehngenii strains, each cataloged under distinct preservation numbers to support a wide range of microbiome research applications. Additional strains can be sourced or developed upon request.
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CAT
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Product Name
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Product Overview
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LBSX-0522-GF58
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Anaerobutyricum soehngenii; 17630
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A. soehngenii is an anaerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from Infant, faecal sample.
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LBSX-0522-GF59
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Anaerobutyricum soehngenii; 109238
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A. soehngenii is an anaerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from pig faeces.
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Customized Formulations
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Culture Supernatant: Tailored preparations of A. soehngenii culture supernatant are available, ideal for evaluating microbial metabolites and host interactions in animal research models.
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Lyophilized Powder: Strain-specific freeze-dried preparations with defined colony-forming units (CFU) can be customized to meet project-specific requirements for controlled research use.
FAQs
Is A. soehngenii difficult to cultivate?
Yes, it is highly oxygen-sensitive, demanding rigorous anaerobic conditions, specialized equipment, and skilled technical expertise, all offered by Creative Biolabs for successful cultivation.
Can this strain be used in co-culture probiotic formulations?
Absolutely. A. soehngenii thrives in beneficial metabolic cross-feeding interactions, making it suitable for carefully designed co-culture probiotic formulations aimed at ecosystem modulation.
Does Creative Biolabs support GRAS dossier preparation for A. soehngenii?
Yes, Creative Biolabs provides comprehensive data packages including strain characterization, safety testing, and functional validation, which are essential for GRAS dossier submissions.
Related Resources
References
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Udayappan, Shanthadevi, et al. "Oral treatment with Eubacterium hallii improves insulin sensitivity in db/db mice." NPJ biofilms and microbiomes 2.1 (2016): 1-10. https://doi.org/10.1038/npjbiofilms.2016.9
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Gilijamse, Pim W., et al. "Treatment with Anaerobutyricum soehngenii: a pilot study of safety and dose–response effects on glucose metabolism in human subjects with metabolic syndrome." npj Biofilms and Microbiomes 6.1 (2020): 16. https://doi.org/10.1038/s41522-020-0127-0
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Shetty, Sudarshan A., et al. "Unravelling lactate‐acetate and sugar conversion into butyrate by intestinal Anaerobutyricum and Anaerostipes species by comparative proteogenomics." Environmental microbiology 22.11 (2020): 4863-4875. https://doi.org/10.1111/1462-2920.15269
